Antibiotic Resistance Profiles of Enterobacteriacea Strains Isolated from Kilis State Hospital Sewage Water

Yıl 2019, Cilt: 31 Sayı: 3, 261 - 266, 01.09.2019

Hatice Aysun Mercimek Takcı , Pemra Bakirhan Bakırhan , Melike Kaptanoglu Sema Genç

https://doi.org/10.7240/jeps.589186

Öz

Enterobacteria strains were isolated from the
sewage water of Kilis State Hospital by using MacConkey medium. A total of 30 bacterial strains were selected
according to their lactose degradation properties. The selected strains were
identified by their morphological characteristics and biochemical activities. Association
with the biochemical tests, 20 strains including Escherichia coli (Biotype I, II), Klebsiella spp and Citrobacter
spp strains were partially identified. Antibiotic resistance profiles were
determined by selecting 8 strains with different activities from 20 isolates. 11
antibiotics with different effect mechanisms were tested against the selected 8
bacterial strains (clindamycin, trimethoprim, chloramphenicol, erythromycin,
tetracycline, streptomycin, ampicillin, cefotaxime, vancomycin, gentamicin and cefoperazone). The MAR index for all tested strains was found to be over 0.2 and the
highest MAR index was 0.75 for Escherichia
coli Biotype I and Citrobacter
spp.

Anahtar Kelimeler

Sewage water, Antibiotic resistance, MAR index, Enterobacteriaceae

Kilis Devlet Hastanesi Kanalizasyon Suyundan İzole Edilen Enterobacteriacea Suşlarının Antibiyotik Dirençlilik Profilleri

Öz

Kilis
Devlet Hastanesi kanalizasyon suyundan MacConkey besi ortamı kullanılarak Enterobacteria familyasına ait suşlar
izole edilmiştir. Laktozu parçalama özelliklerine göre toplamda 30 bakteri suşu
seçilmiştir. Seçilen suşlar morfolojik karakterleri ve biyokimyasal
aktivitelerine göre tanımlanmıştır. Biyokimyasal test sonucuna göre Escherichia coli (Biyotip I ve II)-(Ara
tip I ve II), Klebsiella spp. ve Citrobacter spp. suşları içeren 20 suşun
kısmi tiplendirilmesi yapılmıştır. Tiplendirilen 20 suştan farklı aktiviteler
gösteren 8 suş seçilerek antibiyotik dirençlilik profilleri belirlenmiştir. Seçilen
8 bakteri suşu için farklı etki mekanizmalarına sahip 11 antibiyotik test
edilmiştir (klindamisin, trimethoprim, kloramfenikol, eritromisin, tetrasiklin,
streptomisin, amfisilin, sefotaksim, vankomisin, gentamisin ve sefoperazon). Test
tüm suşlar için MAR indeksi 0.2’nin üzerinde olduğu bulunmuş olup, en yüksek ÇAD
indeksi Escherichia coli Biyotip I ve
Citrobacter spp suşlarında 0.75
olarak hesaplanmıştır.

Anahtar Kelimeler

Kanalizasyon suyu, Antibiyotik dirençlilik, Enterobacteriaceae, ÇAD indeksi

Kaynakça

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