TAURİN, SIÇAN KARACIĞER VE BÖBREK DOKULARINDA 2,3,7,8-TETRAKLORODİBENZO-P-DİOKSİN KAYNAKLI OKSİDATİF STRESİ ÖNLER

Yıl 2023, Cilt: 47 Sayı: 2, 394 - 407, 20.05.2023

Muhammed Fatih Doğan , Osman Çiftçi , Neşe Başak Türkmen , Aslı Çetin , Münevver Nazlıcan Zengin , Bedriye Çiftçi

https://doi.org/10.33483/jfpau.1085253

Öz

Amaç: Çalışmanın amacı, sıçanlarda 2,3,7,8- tetrachlorodibenzo-p-dioksin (TCDD) kaynaklı organ hasarına karşı taurinin önleyici etkilerinin araştırılmasıdır. Çevresel toksin TCDD, hayvan ve insan dokularında yüksek toksisiteye sahiptir. Taurin, birçok organda bulunan bir amino asit olup, antioksidan ve antienflamatuar özellikleri ile hücrelerin korunmasında görev alır. Bu kapsamda, bu çalışmadaki amacımız, TCDD'nin sıçan karaciğer ve böbrek dokularında neden olduğu oksidatif stres ve organ hasarları üzerinde taurinin potansiyel önleyici etkisini araştırmaktır. Bu olası etkileri değerlendirmek üzere, tiyobarbitürik asitle reaksiyona giren reaktif maddeler (TBARS) ve glutatyon (GSH) düzeylerinin yanı sıra süperoksit dismutazın (SOD) aktivitesi ölçülmüştür. Ayrıca, kaspaz-3 ekspresyonunun immünohistokimyasal tespiti ve doku örneklerinde histopatolojik değişikliklerin değerlendirilmesi gerçekleştirilmiştir.
Gereç ve Yöntem: Yetişkin erkek Wistar sıçanları (250-300 g, 12-13 hafta, n = 32) rastgele dört gruba (n = 8/grup) ayrıldı: Kontrol, TCDD, TAU ve TCDD+TAU. TCDD ve/veya taurin gavaj yoluyla sırasıyla 2 μg/kg/hafta ve 200 mg/kg/gün dozlarında uygulandı.
Sonuç ve Tartışma: Bulgular, TCDD'nin GSH düzeyi ve SOD aktivitesini azaltarak ve TBARS düzeylerini artırarak sıçanların karaciğer ve böbrek dokularında oksidatif strese neden olduğunu göstermiştir. Taurin uygulaması, TCDD ve taurinin eşzamanlı uygulamasında TBARS düzeylerinde önemli ölçüde azalma (p<0.05), GSH düzeylerinde ve SOD aktivitesinde ise önemli ölçüde artış sağlamıştır (p<0.05). TCDD'nin karaciğer ve böbrek dokularında neden olduğu oksidatif kaynaklı histopatolojik değişiklikler de taurin uygulaması ile azalmıştır. Taurin, sistein aspartat spesifik proteaz-3'ü (kaspaz-3) azaltarak apoptotik yolağı engelledi. Taurin takviyesi, oksidatif dengesizliği düzenlemeye yardımcı olmakta ve TCDD'nin neden olduğu organ hasarının göstergesi olan histopatolojik değişiklikleri azaltmaktadır. Bu, TCDD toksisitesinden kaçınmak için yeni bir yaklaşım olabilir.

Anahtar Kelimeler

Oksidatif stres, sıçanlar, taurin, TCDD

TAURINE PREVENTS AGAINST 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN-INDUCED OXIDATIVE STRESS IN THE LIVER AND KIDNEY OF RATS

Öz

Objective: The aim of the study was to investigate the preventive effects of taurine against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced organ damage in rats. The environmental toxin TCDD has a high toxicity in animal and human tissues. Taurine is an amino acid found in many organs, with multiple physiological roles including the protection of cells with its antioxidant and anti-inflammatory properties. In this context, our aim in this study was to investigate the potential preventive effect of taurine on oxidative stress and organ damage caused by TCDD in rat liver and kidney tissues. To evaluate these possible effects, we measured the levels of thiobarbituric acid reactive substances (TBARS), and glutathione (GSH), as well as the activity of superoxide dismutase (SOD). In addition, immunohistochemical detection of caspase-3 expression, and the assessment of histopathological changes in tissue samples were performed.
Material and Method: Adult male Wistar rats (250-300 g, 12-13 weeks, n = 32) were randomly allocated into four groups (n = 8/group): Control, TCDD, TAU, and TCDD+TAU. TCDD and/or taurine were administered via gavage in doses of 2 μg/kg/week and 200 mg/kg/day, respectively.
Result and Discussion: The results showed that TCDD caused oxidative stress in the liver and kidney tissues of rats by decreasing the levels of GSH and SOD activity and increasing the levels of TBARS. Taurine treatment significantly reduced TBARS levels (p<0.05), it significantly increased GSH levels and SOD activity (p<0.05) in the concurrent administration of TCDD and taurine. Taurine also reduced the histopathological changes caused by TCDD-induced oxidative stress in the liver and kidney tissues. Taurine prevented the apoptotic pathway by decreasing cysteine aspartate specific protease-3 (caspase-3). Taurine supplementation helps to regulate oxidative imbalance and reduces histopathological changes caused by TCDD-induced organ damage. This could be a novel approach to avoiding TCDD toxicity.

Anahtar Kelimeler

Oxidative stress, rats, taurine, TCDD

Kaynakça

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