COMPARISON OF SOME BIOLOGICAL ACTIVITIES AND CATECHIN TANNIN CONTENTS OF TWO JUNIPERUS AND PRUNUS SPECIES

Yıl 2023, Cilt: 47 Sayı: 2, 650 - 666, 20.05.2023

Hafize Yuca , Hakkı Cem Demircan , Bilge Aydın , Mehmet Önal , Enes Tekman , Ayşe Civaş , Mohaddeseh Nobarirezaeyeh , Gamze Göger , Songül Karakaya , Zuhal Güvenalp

https://doi.org/10.33483/jfpau.1218845

Öz

Objective: Qualitative and quantitative determination of catechin in fruits of J. communis var. saxatilis, J. oxycedrus subsp. oxycedrus, P. spinosa, and P. cerasifera was analyzed by LC-MS/MS using standards. Antidiabetic, antimicrobial, and antioxidant activities of fruit and cone extracts of these plants were evaluated. Qualitative analysis of secondary metabolites was also done.
Material and Method: Antimicrobial activity was done with MIC. ABTS·+ and DPPH• scavenging activies were used antioxidant activity. α-Amylase and α-glucosidase inhibition assays were done for antidiabetic activity.
Result and Discussion: JCS (IC50 = 578 ± 20 μg/ml), JOO (IC50 = 3706 ± 52 μg/ml), and PS (IC50 = 313 ± 7 μg/ml) extracts were observed to show a higher activity than acarbose (IC50 = 4061 ± 146 μg/ml). J. communis var. saxatilis fruit extract was observed with MIC values between (312.5 -2500 µg/ml) against all microorganisms. JCS extract has the highest phenolic composition and JOO has the lowest phenolic composition (µg GAE/ mg extract). JCS extract indicated the highest antioxidant activity. It was determined that plant containing the highest catechin (1173.3±5.77 ng/ml) and epigallocatechin (208±7.21 ng/ml) was JCS. In this research, it can be said that JCS with the highest tannin content shows the highest effects.

Anahtar Kelimeler

Antidiabetic, antimicrobial, catechic, Juniperus, Prunus

Destekleyen Kurum

TUBITAK (2209-A)

Teşekkür

Enes TEKMAN would like to acknowledge the scholarship along with his postgraduate program provided by the Turkish Scientific and Technical Research Council (TUBITAK).

İKİ JUNİPERUS VE PRUNUS TÜRÜNÜN BAZI BİYOLOJİK AKTİVİTELERİNİN VE KATEŞİN TANEN İÇERİKLERİNİN KARŞILAŞTIRILMASI

Öz

Amaç: J. communis var. saxatilis, J. oxycedrus subsp. oxycedrus, P. spinosa, ve P. cerasifera meyvelerinde standartlar kullanılarak LC-MS/MS ile analiz edilmiştir. Bitkilerin meyve ve kozalak ekstrelerinin antidiyabetik, antimikrobiyal ve antioksidan aktiviteleri değerlendirilmiştir. Sekonder metabolitlerin kalitatif analizi de yapılmıştır.
Gereç ve Yöntem: MIC ile antimikrobiyal aktivite yapıldı. ABTS·+ ve DPPH• süpürücü testler antioksidan aktiviteler için kullanılmıştır. α-Amilaz ve α-glukosidaz inhibisyon testleri antidiyabetik aktivite için kullanılmıştır.
Sonuç ve Tartışma: JCS (IC50 = 578 ± 20 μg/ml), JOO (IC50 = 3706 ± 52 μg/ml) ve PS (IC50 = 313 ± 7 μg/ml) ekstraktlarının akarbozdan (IC50 = 4061 ± 146 μg/ml) daha yüksek aktivite gösterdiği gözlemlenmiştir. Juniperus communis var. saxatilis meyve ekstresi tüm mikroorganizmalara karşı (312.5 -2500 µg/ml) arasında MİK değerleri göstermiştir. JCS en yüksek fenolik bileşime ve JOO en düşük fenolik bileşime sahiptir (µg GAE/ mg ekstrakt). JCS ekstresi en yüksek antioksidan aktiviteyi göstermiştir. En yüksek kateşin (1173.3±5.77 ng/ml) ve epigallokateşin (208±7.21 ng/ml) içeren bitki JCS’dir. Bu araştırmada en yüksek tanen içeriğine sahip JCS’nin en yüksek etkileri gösterdiği söylenebilir.

Anahtar Kelimeler

Antidiyabetik, antimikrobiyal, kateşik, Juniperus, Prunus

Kaynakça

• 1. Wickramasinghe, A.S.D., Kalansuriya, P., Attanayake, A.P. (2022). Nanoformulation of plant-based natural products for type 2 diabetes mellitus: From formulation design to therapeutic applications. Current Therapeutic Research, 96, 100672. [CrossRef]
• 2. Akash, M.S.H., Rehman, K., Fiayyaz, F., Sabir, S., Khurshid, M. (2020). Diabetes-associated infections: Development of antimicrobial resistance and possible treatment strategies. Archives of Microbiology, 202, 953-965. [CrossRef]
• 3. Sivanmaliappan, T.S., Sevanan, M. (2011). Antimicrobial susceptibility patterns of Pseudomonas aeruginosa from diabetes patients with foot ulcers. International Journal of Microbiology, 2011, 605195. [CrossRef]
• 4. Wei, S., Xu, P., Yao, Z., Cui, X., Lei, X., Li, L., Dong, L., Zhu, W., Guo, R., Cheng, B. (2021). A composite hydrogel with co-delivery of antimicrobial peptides and platelet-rich plasma to enhance healing of infected wounds in diabetes. Acta Biomaterialia, 124, 205-218. [CrossRef]
• 5. Scott, J.A., King, G.L. (2004). Oxidative stress and antioxidant treatment in diabetes. Annals of the New York Academy of Sciences, 1031(1), 204-213. [CrossRef]
• 6. Dembinska-Kiec, A., Mykkänen, O., Kiec-Wilk, B., Mykkänen, H. (2008). Antioxidant phytochemicals against type 2 diabetes. British Journal of Nutrition, 99(E-S1), ES109-ES117. [CrossRef]
• 7. Kumari, M., Jain, S. (2012). Tannins: An antinutrient with positive effect to manage diabetes. Research Journal of Recent Sciences, 1(12), 1-8.
• 8. Laddha, A.P., Kulkarni, Y.A. (2019). Tannins and vascular complications of Diabetes: An update. Phytomedicine, 56, 229-245. [CrossRef]
• 9. Bais, S., Gill, N.S., Rana, N., Shandil, S. (2014). A phytopharmacological review on a medicinal plant: Juniperus communis. International Scholarly Research Notices, 1-6. [CrossRef]
• 10. Karaman, I., Şahin, F., Güllüce, M., Öǧütçü, H., Şengül, M., Adıgüzel, A. (2003). Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. Journal of Ethnopharmacology, 85 (2-3), 231-235. [CrossRef]
• 11. Veličković, I., Žižak, Ž., Rajčević, N., Ivanov, M., Soković, M., Marin, P., Grujic, S. (2020). Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts. Archives of Biological Sciences, 72 (1), 105-115. [CrossRef]
• 12. Ruiz-Rodríguez, B.M., de Ancos, B., Sánchez-Moreno, C., Fernández-Ruiz, V., de Cortes Sánchez-Mata, M., Cámara, M., Tardío, J. (2014). Wild blackthorn (Prunus spinosa L.) and hawthorn (Crataegus monogyna Jacq.) fruits as valuable sources of antioxidants. Fruits, 69(1), 61-73. [CrossRef]
• 13. Khadivi, A., Mirheidari, F., Moradi, Y., Paryan, S. (2020). Phenotypic and fruit characterizations of Prunus divaricata Ledeb. germplasm from the north of Iran. Scientia Horticulturae, 261, 109033. [CrossRef]
• 14. Arıtuluk, Z.C., Ezer, N. (2012). Halk arasında diyabete karşı kullanılan bitkiler (Türkiye)-II. Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, 32 (2), 179-208.
• 15. Nampoothiri, S.V., Prathapan, A., Cherian, O.L., Raghu, K.G., Venugopalan, V.V., Sundaresan, A. (2011). In vitro antioxidant and inhibitory potential of Terminalia bellerica and Emblica officinalis fruits against LDL oxidation and key enzymes linked to type 2 diabetes. Food and Chemical Toxicology, 49(1), 125-131. [CrossRef]
• 16. Yuca, H., Özbek, H., Demirezer, L.Ö., Kasil, H.G., Güvenalp, Z. (2021). Trans-tiliroside: A potent α-glucosidase inhibitor from the leaves of Elaeagnus angustifolia L. Phytochemistry, 188, 112795. [CrossRef]
• 17. Bachhawat, J.A., Shihabudeen, M.S., Thirumurugan, K. (2011). Screening of fifteen Indian ayurvedic plants for alpha-glucosidase inhibitory activity and enzyme kinetics. International Journal of Pharmacy and Pharmaceutical Sciences, 3(4), 267-74.
• 18. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26 (9-10), 1231-1237. [CrossRef]
• 19. Aydın, B., Yuca, H., Karakaya, S., Bona, G.E., Göger, G., Tekman, E., Şahin, A.A., Sytar, O., Civas, A., Canlı, D., Pınar, N.M., Guvenalp Z. (2023). The anatomical, morphological features, and biological activity of Scilla siberica subsp. armena (Grossh.) Mordak (Asparagaceae). Protoplasma, 260, 371-389. [CrossRef]
• 20. Sevindik, H.G., Ozek, T., Yerdelen, K.O., Onal, M., Ozbek, H., Guvenalp, Z., Demirezer L.Ö. (2016). Chemical composition, antioxidant capacity, acetyl- and butyrylcholinesterase inhibitory activities of the essential oil of Thymus haussknechtii Velen. Records of Natural Products, 10(4), 503-507.
• 21. Folin, O., Denis, W. (1912). On phosphotungstic-phosphomolybdic compounds as color reagents. Journal of Biological Chemistry, 12(2), 239-243. [CrossRef]
• 22. Slinkard, K., Singleton, V.L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28 (1), 49-55.
• 23. Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200. [CrossRef]
• 24. Karakaya, S. (2016). PhD Thesis. Investigations on Ferulago trachycarpa Boiss., F. blancheana Post., F. pachyloba (Fenzl) Boiss. and F. bracteata Boiss. & Haussk. (Apiaceae). Department of Pharmaceutical Botany, Faculty of Pharmacy, Ankara University, Ankara, Turkey.
• 25. Horn, S.W.C., Lewis, M., Palmer, M.R., Bayse, C.A. (2020). Examination of the composition and mechanism of discoloration of the fugitive pigment copper resinate. Inorganica Chimica Acta, 504, 119407. [CrossRef]
• 26. Popović, B.M., Blagojević, B., Kucharska, A.Z., Agić, D., Magazin, N., Milović, M., Serra, A.T. (2021). Exploring fruits from genus Prunus as a source of potential pharmaceutical agents-In vitro and in silico study. Food Chemistry, 358, 129812. [CrossRef]
• 27. Orhan, N., Hoçbaç, S., Orhan, D.D., Asian, M., Ergun, F. (2014). Enzyme inhibitory and radical scavenging effects of some antidiabetic plants of Turkey. Iranian Journal of Basic Medical Sciences, 17(6), 426.
• 28. Miceli, N., Trovato, A., Dugo, P., Cacciola, F., Donato, P., Marino, A., Bellinghieri, V., La Barbera, T.M., Güvenç, A., Taviano, F.M. (2009). Comparative analysis of flavonoid profile, antioxidant and antimicrobial activity of the berries of Juniperus communis L. var. communis and Juniperus communis L. var. saxatilis Pall. from Turkey. Journal of Agricultural and Food Chemistry, 57(15), 6570-6577. [CrossRef]
• 29. Taviano, M.F., Marino, A., Trovato, A., Bellinghieri, V., La Barbera, T.M., Güvenç, A., Hürkül M.M., De Pasquale, R., Miceli, N. (2011). Antioxidant and antimicrobial activities of branches extracts of five Juniperus species from Turkey. Pharmaceutical Biology, 49(10), 1014-1022. [CrossRef]
• 30. Öztürk, M., Tümen, İ., Uǧur, A., Aydoǧmuş‐Öztürk, F., Topçu, G. (2011). Evaluation of fruit extracts of six Turkish Juniperus species for their antioxidant, anticholinesterase and antimicrobial activities. Journal of the Science of Food and Agriculture, 91(5), 867-876. [CrossRef]
• 31. Ceylan, O., Sahin, M.D., Avaz, S. (2013). Antibacterial activity of Corylus colurna L. (Betulaceae) and Prunus divaricata Ledep. subsp. divaricata (Rosaceae) from Usak, Turkey. Bulgarian Journal of Agricultural Science, 19(6), 1204-1207.
• 32. Çömlekcioğlu, N., Kocabaş, Y.Z., Aygan, A. (2020). Determination of biochemical composition and antimicrobial activities of Prunus divaricata subsp. divaricata Ledeb. fruits collected from Kahramanmaraş. Anadolu, 30(1), 46-56. [CrossRef]
• 33. Sabatini, L., Fraternale, D., Di Giacomo, B., Mari, M., Albertini, M.C., Gordillo, B. Rocchi, M.B.L., Sisti, D., Coppari, S., Semprucci, F., Guidi, L., Colomba, M. (2020). Chemical composition, antioxidant, antimicrobial and anti-inflammatory activity of Prunus spinosa L. fruit ethanol extract. Journal of Functional Foods, 67, 103885. [CrossRef]
• 34. Phaniendra, A., Jestadi, D.B., Periyasamy, L. (2015). Free radicals: Properties, sources, targets, and their implication in various diseases. Indian Journal of Clinical Biochemistry, 30(1), 11-26. [CrossRef]
• 35. Elmastaş, M., Gülçin, I., Beydemir, Ş., İrfan Küfrevioğlu, Ö., Aboul‐Enein, H.Y. (2006). A study on the in vitro antioxidant activity of juniper (Juniperus communis L.) fruit extracts. Analytical Letters 2006, 39(1), 47-65. [CrossRef]
• 36. Celik, F., Gundogdu, M., Alp, S., Muradoglu, F., Ercişli, S., Geçer, M.K., Canan, I. (2017). Determination of phenolic compounds, antioxidant capacity and organic acids contents of Prunus domestica L., Prunus cerasifera Ehrh. and Prunus spinosa L. fruits by HPLC. Acta Chromatographica, 29(4), 507-510. [CrossRef]
• 37. Pinacho, R., Cavero, R.Y., Astiasarán, I., Ansorena, D., Calvo, M.I. (2015). Phenolic compounds of blackthorn (Prunus spinosa L.) and influence of in vitro digestion on their antioxidant capacity. Journal of Functional Foods, 19, 49-62. [CrossRef]
• 38. Yaglioglu, A.S., Eser, F. (2017). Screening of some Juniperus extracts for the phenolic compounds and their antiproliferative activities. South African Journal of Botany, 113, 29-33. [CrossRef]
• 39. Gonçalves, A.C., Flores-Félix, J.D., Coutinho, P., Alves, G., Silva, L.R. (2022). Zimbro (Juniperus communis L.) as a promising source of bioactive compounds and biomedical activities: A review on recent trends. International Journal of Molecular Sciences, 23(6), 3197. [CrossRef]