FENİTOİNİN YEŞİL HPLC TAYİNİ VE METOT VALİDASYONU

Yıl 2024, Cilt: 48 Sayı: 3, 1021 - 1032, 10.09.2024

Ertuğrul Faruk Ökmen , Ebru Çubuk Demiralay , İlkay Konçe , Yaşar Doğan Daldal

https://doi.org/10.33483/jfpau.1454525

Öz

Amaç: Bu çalışmada antiepileptik ilaç fenitoinin kromatografik davranışı yeşil HPLC yöntemi ile belirlenmiştir. Geliştirilen yöntemin optimizasyonu, değişen su-etanol ikili karışımlarındaki fenitoinin kapasite faktörü değerlerine ve bileşiğin analiz edildiği mobil fazdaki etanol derişimine dayandırılmıştır.
Gereç ve Yöntem: RPLC yöntemiyle geliştirilen metot optimizasyonunda %35, %40 ve %45 (h/h) etanol içeren etanol-su ikili karışımları kullanılmıştır. Bileşiğin alıkonma süreleri Zorbax SB-CN (150x4.6 mm, 3.5 µm ID) kolonu ile belirlenmiştir. Analizler sabit akış hızı (0.3 ml/dakika) ve kolon sıcaklığında (37°C) gerçekleştirilmiştir. Kantitatif analiz için optimum koşul, pH'ı 6.5 olan %40 (h/h) etanol içeren bir etanol-su ikili karışımı olarak belirlenmiştir.
Sonuç ve Tartışma: Bu çalışmada logk-φ ilişkisini kullanılarak fenitoinin hidrofobisitesi hesaplanmıştır. Elde edilen kromatografik veriler kullanılarak optimum koşul belirlenmiş, ticari tablet formülasyonunda fenitoinin kantitatif tayini dahili standart yöntemiyle yapılmıştır. Bu koşullar altında 0.8-2.8 μg/ml derişim aralığında mükemmel doğrusallık (r>0.99) elde edilmiştir. Geliştirilen yöntemin tespit limiti 0.021 μg/ml; miktar belirleme sınırı 0.064 μg/ml olarak hesaplanmıştır. Yöntemin geri kazanım değeri %99.61 olarak belirlenmiştir. Kesinlik, doğruluk ve metot sağlamlığı parametrelerinin validasyon prosedürleri için uygun olduğu sonucuna varılmıştır.

Anahtar Kelimeler

Antiepileptik ilaçlar, ikili karışım, RPLC, yeşil kimya, yöntem optimizasyonu

GREEN HPLC DETERMINATION OF PHENYTOIN AND METHOD VALIDATION

Öz

Objective: In this study, the chromatographic behavior of the antiepileptic drug phenytoin was determined by the green HPLC method. The optimization of the developed method was based on the capacity factor values of phenytoin in varying water-ethanol binary mixtures and the ethanol concentration in the mobile phase where the compound was analyzed.
Material and Method: Ethanol-water binary mixtures containing 35%, 40%, and 45% (v/v) ethanol were used in the optimization for the determination performed by the RPLC method. Retention times of the compound were determined with the Zorbax SB-CN (150x4.6 mm, 3.5 µm ID) column. Analyzes were performed at a constant flow rate (0.3 ml/min) and column temperature (37°C). The optimum condition for quantitative analysis was determined as an ethanol-water binary mixture containing 40% (v/v) ethanol with a pH of 6.5.
Result and Discussion: In this study, the hydrophobicity of phenytoin was calculated using the logk-φ relationship. The optimum condition was determined using the obtained chromatographic data, and the quantitative determination of phenytoin in the commercial tablet formulation was made by the internal standard method. Under these conditions, excellent linearity (r>0.99) was obtained in the concentration range of 0.8-2.8 μg/ml. The detection limit of the developed method is 0.021 μg/ml; the limit of quantitation was calculated as 0.064 μg/ml. The recovery value of the method was determined as 99.61%. It was concluded that the parameters of precision, accuracy, and method robustness were appropriate for the validation procedures.

Anahtar Kelimeler

Antiepileptic drugs, binary mixture, green chemistry, method optimization, RPLC

Etik Beyan

None

Kaynakça

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