Novel microbiome friendly purifying oil cleanser formulation with oil-soluble postbiotics

Yıl 2024, Cilt: 9 Sayı: 4, 138 - 147, 14.02.2025

Leyla Tarhan Çelebi , Ertugrul Bursalıoğlu , Bekir Çakıcı , Mehtap Arslan Aydin , Ahmet Arif Kurt , Bashar Ibrahim , İsmail Aslan

https://doi.org/10.58854/jicm.1580770

Öz

Objective: In recent years, the field of preventive cosmetic care has gained importance alongside the established effectiveness of nutrition in preventing diseases. Within the scope of this preventive approach, the microbiota has been a focal point in numerous studies, with its state being associated with a range of health concerns, including inflammation, metabolic diseases, dermatological diseases, and neurodegenerative diseases.The present study aims to investigate the positive effects of a cleanser formulated with a postbiotic raw material dissolved in oil on skin microbiota and cells. In this study, the skin cleansing oil containing 2% postbiotic LTO 35 was utilised as a skin microbiota simulation (Lactobacillus crispatus LTC-KC24011, Staphylococcus epidermidis ATA-LSE 0198052) and normal keratinocytes (HEKA 500K CELL), Staphylococcus aureus ATA-LTCA 011204, Staphylococcus capitis ATA-LSC 0201201, Propionibacterium acnes ATA-LPC 0204221, Streptococcus pyogenes ATA-TCSP 210911, and Candida albicans ATA-LTCA 0504212. The results demonstrated that the cleanser did not cause harm to keratinocytes, maintained microbiota balance, and promoted cellular repair.The objective of this study was to investigate the positive effects of a cleanser made using oil-solubilised postbiotic raw material on skin microbiota and cells.
Material&Method: In this study, a skin cleansing oil containing 2% postbiotic LTO 35 was utilised to simulate normal keratinocytes (HEKA 500K CELL) and skin microbiota (Lactobacillus crispatus LTC-KC24011, Staphylococcus epidermidis ATA-LSE 0198052, Staphylococcus aureus ATA -LTCA 011204, Staphylococcus capitis ATA-LSC 0201201, Propionibacterium acnes ATA-LPC 0204221, Streptococcus pyogenes ATA TCSP 210911, Candida albicans ATA-LTCA 0504212. The bacteria utilised in the simulation and the postbiotic employed in the formulation (LTO 35 Streptococcus thermophilus ATA-LTC St140700, Bifidobacterium animalis ATA-BSLA0310, Lactobacillus acidophilus ATA-LAP1201 ferment extract in oil lysate) were obtained from the ATA BIO Technology culture collection. The simulation contact time was applied as 1 hour.
Results: The Purifying Oil Cleanser with Postbiotics sample examined in this study was found to be microbiologically suitable. The equilibrium test yielded a positive result, and it was observed that the sample did not change in the direction of pathogens. According to the analyses, it can be concluded that the Purifying Oil Cleanser with Postbiotics sample does not harm the diversity of skin microbiota and is microbiome-friendly.
Conclusion: Upon evaluation of the results, it was observed that the substance did not cause harm to keratinocytes, it maintained microbiota balance, and it promoted cellular repair.

Anahtar Kelimeler

Probiotic, Postbiotic, Microbiota, Oil Cleanser, Cosmetics

Etik Beyan

-

Destekleyen Kurum

-

Proje Numarası

-

Teşekkür

-

Yağda çözünen postbiyotiklerle yeni mikrobiyom dostu temizleyici yağ formülasyonu

Öz

Amaç: Son yıllarda hastalıklardan korunmada beslenmenin etkinliğinin yanında koruyucu kozmetik bakım da giderek önem kazanmıştır. Koruyucu yaklaşımda özellikle mikrobiyotanın etkinliği pek çok çalışmada yer almıştır. Mikrobiyotanın durumu, vücuttaki enflamasyon, çeşitli metabolik hastalıklar, dermal hastalıklar, nörodejeneratif hastalıklar veya dermal hastalıklarla ilişkilendirilmiştir. Bu çalışmada, yağ içinde çözündürülmüş postbiyotik hammadde kullanılarak yapılan temizleyicinin cilt mikrobiyotasına ve hücre üzerine olumlu etkilerinin araştırılması amaçlanmıştır. Bu çalışmada, %2 postbiyotik LTO 35 içeren cilt temizleyici yağın, normal keratinositler (HEKA 500K CELL) ve cilt mikrobiyota simülasyonu (Lactobacillus crispatus LTC-KC24011, Staphylococcus epidermidis ATA-LSE 0198052, Staphylococcus aureus ATA-LTCA 011204, Staphylococcus capitis ATA -LSC 0201201, Propionibacterium acnes ATA-LPC 0204221, Streptococcus pyogenes ATA TCSP 210911, Candida albicans ATA-LTCA 0504212) üzerindeki etkileri değerlendirilmiştir. Sonuçlar değerlendirildiğinde, keratinositlere zarar vermediği, mikrobiyota dengesini koruduğu ve hücresel onarıma destek olduğu görülmüştür. Bu çalışmada, yağ içinde çözündürülmüş postbiyotik hammadde kullanılarak yapılan temizleyicinin cilt mikrobiyotasına ve hücre üzerine olumlu etkilerinin araştırılması amaçlanmıştır.
Yöntem: Bu çalışmada, %2 postbiyotik LTO 35 içeren cilt temizleyici yağın, normal keratinositler (HEKA 500K CELL) ve cilt mikrobiyota simülasyonu (Lactobacillus crispatus LTC-KC24011, Staphylococcus epidermidis ATA-LSE 0198052, Staphylococcus aureus ATA-LTCA 011204, Staphylococcus capitis ATA -LSC 0201201, Propionibacterium acnes ATA-LPC 0204221, Streptococcus pyogenes ATA TCSP 210911, Candida albicans ATA-LTCA 0504212) kullanılmıştır. Simülasyonda kullanılan bakteriler ve formülasyon içinde kullanılan postbiyotik (LTO 35 Streptococcus thermophilus ATA-LTC St140700, Bifidobacterium animalis ATA-BSLA0310, Lactobacillus acidophilus ATA-LAP1201 ferment ekstrakt in oil lizat) ATA BIO Teknoloji kültür koleksiyonundan temin edilmiştir. Simülasyon kontak süresi 1 saat olarak uygulanmıştır.
Bulgular: Bu çalışmada kullanılan Purifying Oil Cleanser with Postbiotics numunesi, mikrobiyolojik açıdan uygun olarak değerlendirilmiştir. Denge testi olumlu bulunup, patojen yönünde değişmediği gözlenmiştir. Yapılan analizlere göre; Purifying Oil Cleanser with Postbiotics numunesinin cilt mikrobiyotası çeşitliliğine zarar vermediği ve mikrobiyom dostu olduğu ifade edilebilir.
Sonuç: Sonuçlar değerlendirildiğinde, keratinositlere zarar vermediği, mikrobiyota dengesini koruduğu ve hücresel onarıma destek olduğu görülmüştür.

Anahtar Kelimeler

Probiyotik, Postbiyotik, Mikrobiyota, Yağ Temizleyici, Kozmetik

Proje Numarası

-

Kaynakça

• Prescott SL, Larcombe DL, Logan AC, West C, Burks W, Caraballo L, et al. The skin microbiome: impact of modern environments on skin ecology, barrier integrity, and systemic immune programming. World Allergy Organ J. 2017;10:1-16.
• Roy S, Dhaneshwar S. Role of prebiotics, probiotics, and synbiotics in management of inflammatory bowel disease: Current perspectives. World J Gastroenterol. 2023;29(14):2078.
• Zhao X, Liu S, Li S, Jiang W, Wang J, Xiao J, et al. Unlocking the power of postbiotics: A revolutionary approach to nutrition for humans and animals. Cell Metab. 2024;36(4):725-44.
• Aguilar-Toalá JE, Garcia-Varela R, Garcia HS, Mata-Haro V, González-Córdova AF, Vallejo-Cordoba B, et al. Postbiotics: An evolving term within the functional foods field. Trends Food Sci Technol. 2018;75:105-14.
• Taverniti V, Guglielmetti S. The immunomodulatory properties of probiotic microorganisms beyond their viability (ghost probiotics: proposal of paraprobiotic concept). Genes Nutr. 2011;6(3):261-74.
• Patel RM, Denning PW. Therapeutic use of prebiotics, probiotics, and postbiotics to prevent necrotizing enterocolitis: what is the current evidence?. Clin Perinatol. 2013;40(1):11-25.
• Sionek B, Gantner M. Probiotics and Paraprobiotics—New Proposal for Functional Food. Appl Sci. 2025;15(1).
• Oleskin AV, Shenderov BA. Microbial Communication and Microbiota-Host Interactivity: Neurophysio-logical, Biotechnological, and Biopolitical Implications. Hauppauge, NY: Nova Science Publishers; 2020.
• Kothari D, Patel S, Kim SK. Probiotic supplements might not be universally effective and safe: A review. Biomed Pharmacother. 2019;111:537-47.
• Aslan İ, Tarhan Çelebi L. Postbiotics Cosmetic Formulation: In Vitro Efficacy Studies on a Microbiome Friendly Antiperspirant. J Res Pharm. 2023;27(5).
• Gökçe HB, Aslan İ. Novel Liposome–Gel Formulations Containing a Next Generation Postbiotic: Charac-terization, Rheological, Stability, Release Kinetic, and In Vitro Antimicrobial Activity Studies. Gels. 2024;10(11):746.
• Bursalıoğlu E, Tarhan L, Çakıcı B, Genel N, Kalbişen HT, Aslan İ. Can buttermilk (ayran) with its postbiotic content be used in the treatment of colon cancer?. J Immunol Clin Microbiol. 2024;9(4):130-40.
• Aslan İ, Kurt AA. Characterization and Optimization of Phytosome Formulation Containing Alcohol-free Umckalin from Pelargonium sidoides. Curr Perspect Med Aromat Plants. 2020;3(1):49-53.
• Aslan İ, Kurt AA. In-vitro comparison release study of novel liposome and conventional formulation containing Rosmarinus officinalis extract. Curr Perspect Med Aromat Plants. 2021;4(1):13-21.
• Kurt AA, Ibrahim B. Preparation of New Generation Natural Repellent Formulations From Essential Oils: From Basic Research to Technological Development. J Cosmet Sci. 2024;75(4).
• Ibrahim B, Kurt AA. Mikrobiyolojik Olarak Test Edilmiş Bitkisel Ekstraktlar ve Esansiyel Yağlar ile Saç Dö-külmesine Karşı Doğal Şampuan Formülasyon Geliştirilmesi. J Immunol Clin Microbiol. 2024;9(1):12-23.
• Kurt AA, Ibrahim B. New generation natural face cream formulation: development and in vitro evalua-tion. Celal Bayar Univ Sag Bilim Enst Derg. 2024;11(4):672-80.
• Kurt AA, Aslan I, Duman G. Next-Generation Natural Baby Barrier Cream Formulations: Physicochemical Analysis and Safety Assessment. J Cosmet Sci. 2021;72(2).
• Bursalioğlu EO. Evaluation of antibacterial activity of Triticum monococcum seeds, Castanea sativa se-eds and Begonia maculata leaves against several bacterial strains. Turk J Biodiv. 2020;3:9-14.
• Buran K. Design, Synthesis, Biological Evaluation and Docking, ADME Studies of Novel Phenylsulfonyl Piperazine Analogues as α-Amylase Inhibitors. Cumhuriyet Sci J. 2024;45(2):268-73.
• Buran K, İnan Y, Akyüz GS, Özdemir CD, Kocabas F. Phenylsulfonylpiperazines as α-Glucosidase Enzyme Inhibitors: Design, Synthesis, DFT Calculations, Docking and ADME Studies. Bitlis Eren Univ Fen Bilim Derg. 2024;13(3):723-30.
• Buran K. Benzoin-tryptamine Schiff base-metal complexes: synthesis, DFT calculation and antimicrobial activities. J Mol Struct. 2025;141499.
• Grice EA, Segre JA. The skin microbiome. Nat Rev Microbiol. 2011;9(4):244-53.
• Barrandon Y, Green H. Three clonal types of keratinocyte with different capacities for multiplication. Proc Natl Acad Sci U S A. 1987;84(8):2302-6.
• Schmidt-Ullrich R, Paus R. Molecular principles of hair follicle induction and morphogenesis. Bioessays. 2005;27(3):247-61.
• Tickle C. Making digit patterns in the vertebrate limb. Nat Rev Mol Cell Biol. 2006;7(1):45-53.
• Panati C. Panati’s extraordinary origins of everyday things. Chartwell Books; 2016.
• A framework for human microbiome research. Nature. 2012;486(7402):215-21.
• Ajayi SA, Olaniyi OO, Oladoyinbo TO, Ajayi ND, Olaniyi FC. Sustainable Sourcing of Organic Skincare Ing-redients: A Critical Analysis of Ethical Concerns and Environmental Implications. Asian J Adv Res Rep. 2024;18(1):65-91.
• Prescott SL, Larcombe DL, Logan AC, West C, Burks W, et al. The skin microbiome: impact of modern environments on skin ecology, barrier integrity, and systemic immune programming. World Allergy Or-gan J. 2019;10:29.
• Silva Vale A, Melo Pereira G, Oliveira AC, Carvalho Neto DP, et al. Production, Formulation, and Applica-tion of Postbiotics in the Treatment of Skin Conditions. Fermentation. 2023;9(3):264.
• Sanford JA, Zhang LJ, Williams MR, Gangoiti JA, Huang CM, Gallo RL. Short-chain fatty acids from Cuti-bacterium acnes activate both a canonical and epigenetic inflammatory response in human sebocytes. J Immunol. 2019;202(6):1767-76.
• Aslan I, Tarhan Celebi L, Kayhan H, Kizilay E, Gulbahar MY, Kurt H, et al. Probiotic formulations contai-ning fixed and essential oils ameliorates SIBO-Induced gut dysbiosis in rats. Pharmaceuticals. 2023;16(7):1041.