Evaluation of Physiological and Biochemical Responses against to Salinity in Local Rice (Oryza sativa L.) under in vitro Conditions

Yıl 2020, Cilt: 10 Sayı: 2, 828 - 837, 01.06.2020

Mehmet Yusuf Orcan , Filiz Akbaş

https://doi.org/10.21597/jist.611777

Öz

In a local rice cultivar (Karacadağ), physiological and biochemical changes caused by different salt types (NaCI, CaCI2, MgCI2) and their concentrations (25, 50, 75, 150, 300 mM) were investigated under stress conditions. The germination percentage was not affected at low concentrations in each of 3 types salt tested but it decreased significantly as the concentration increased. In development stages of seedling, it was determined that as the concentration increased in all salt types, plant growth and relative water content (RWC) decreased. Salinity was mostly affected the photosynthetic pigment contents of the plants and there was a difference significantly between the results according to the salt concentration. In general, it was determined that malondialdehyde (MDA) content increased depending on the concentration in all salt types that tested. The highest cell membrane damage was found in the 75 mM application of CaCI2 with 4.1820 μmol /g MDA. As a result, it was determined that germination and seedling development stages were negatively affected in the salt types tested, especially at high concentrations.

Anahtar Kelimeler

Rice, Karacadağ, salt stress, in vitro

Destekleyen Kurum

Batman Üniversitesi

Proje Numarası

BTÜBAP-2016-Yüksek Lisans-9

Teşekkür

This work is part of the BTÜBAP-2016-Yüksek Lisans-9 project financially supported by Batman University-Scientific Research Projects Coordinator the Ministry of Science. We express our sincerest gratitude to them for their support.

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