Bazı Antibiyotiklerin Biyofilm Oluşturan Stafilokok İzolatları Üzerindeki Etkilerinin Değerlendirilmesi

Yıl 2020, Cilt: 10 Sayı: 2, 838 - 845, 01.06.2020

Sevim Feyza Erdoğmuş , Selahattin Konak

https://doi.org/10.21597/jist.615352

Cited By: 1

Öz

Stafilokoklar çeşitli virülans faktörlere sahip olan patojen mikroorganizmalardır. Bu virülans faktörlerden birisi biyofilm üretimidir. Bazı stafilokoklar, biyofilm üreterek antibiyotiklere karşı dirençli hale gelmektedir ve enfeksiyonların tedavisi zorlaşmaktadır. Bu çalışmanın amacı, daha önce bir çalışmada mastitisli inek sütlerinden izole edilmiş olan stafilokok izolatlarının planktonik ve biyofilm oluşturan formlarının üzerine vankomisin, eritromisin, oksasilin antibiyotiklerinin etkisini belirlemektir. Antibiyotiklerin minimum inhibitör konsantrasyonu (MİK), biyofilm minimum inhibitör konsantrasyonu (MBİK) ve minimum biyofilm eradikasyon konsantrasyonu (MBEK) değerleri belirlenmiştir. Antibiyotiklerin MİK, MBİK ve MBEK değerleri karşılaştırıldığında; en yüksek MİK değeri (32-64 μg ml-1) eritromisin için olduğu belirlenmiştir. MBİK ve MBEK değerleri ise tüm antibiyotikler için benzerlik göstermiştir. Bu çalışmanın sonucunda, stafilokok izolatlarının biyofilm oluşturarak antibiyotiklere karşı daha dirençli hale geldikleri ve serbest yaşayan formlarına göre daha yüksek dozda antibiyotiklerden etkilendikleri saptanmıştır.

Anahtar Kelimeler

Antibiyotik direnci, biyofilm, stafilokok

Destekleyen Kurum

AKÜ BAP

Proje Numarası

17. KARİYER. 91

Teşekkür

Bu çalışma, AKÜ BAP 17. KARİYER. 91 no’lu proje tarafından desteklemiştir.

Evaluation of the Effects of Some Antibiotics on Biofilm Forming Staphylococci Isolates

Öz

Staphylococci are pathogenic microorganisms that have various virulence factors. One of the most important of these virulence factors is biofilm production. Some staphylococci become resistant to antibiotics by producing biofilms and treatment of infections becomes more difficult. The aim of this study was to determine the effect of vancomycin, erythromycin, oxacillin antibiotics on planktonic and biofilm forming forms of staphylococci isolates which were isolated from mastitis cow's milk in a previous study. Minimum inhibitory concentration (MIC), minimum biofilm inhibitory concentration (MBIC), minimum biofilm eradication concentration (MBEC) values of antibiotics were determined. When the MIC, MBIC and MBEC values of antibiotics were compared; the highest range of MIC values was determined for erythromycin (32-64 μg ml-1). MBIC and MBEC values were similar for all test antibiotics. As a result of this study, staphylococci isolates were found to be more resistant to antibiotics by producing biofilm and were affected by higher doses of antibiotics than their free-living forms.

Anahtar Kelimeler

Antibiotic resistance, biofilm, staphylococci

Proje Numarası

17. KARİYER. 91

Kaynakça

• Akan E, Kınık Ö, 2014. Biyofilm Oluşum Mekanizması ve Biyofilmlerin Gıda Güvenliğine Etkisi. Gıda ve Yem Bilimi Teknolojisi Dergisi, 14: 42-51.
• Altun HU, Şener B, 2008. Biyofilm İnfeksiyonları ve Antibiyotik Direnci. Hacettepe Tıp Dergisi, 39: 82-88.
• Aslantaş Ö, Demir C, 2016. Investigation of the Antibiotic Resistance and Biofilm-Forming Ability of Staphylococcus aureus from Subclinical Bovine Mastitis Cases. Journal of Dairy Science, 99 (11): 8607-8613.
• Bardiaua M, Caplinb J, Detilleuxc J, Graberd H, Moronie P, Taminiaug B, Mainila JG, 2016. Existence of Two Groups of Staphylococcus aureus Strains Isolated from Bovine Mastitis Based on Biofilm Formation, Intracellular Survival, Capsular Profile and Agr-typing, Veterinary Microbiology, 185: 1-6.
• Belbase A, Pant ND, Nepal K, Neupane B, Baidhya R, Baidya R, Lekhak B, 2017. Antibiotic Resistance and Biofilm Production Among the Strains of Staphylococcus aureus Isolated from Pus/Wound Swab Samples in a Tertiary Care Hospital in Nepal, Annals of Clinical Microbiology and Antimicrobials, Doi: 10.1186/s12941-017-0194-0.
• Bendahou A, Lebbadi M, Ennanei L, Essadqui FZ, Abid M, 2008. Characterization of Staphylococcus Species Isolated from Raw Milk and Milk Products in North Morocco, Journal of Infection in Developing Countries, 2(3): 225-218.
• CLSI (Clinical and Laboratory Standards Institute), 2016. Performance Standards for Antimicrobial Susceptibility Testing; 26 th Edition, CLSI Supplement M100S, Wayne, PA.
• Cucarella C, Tormo MA, Beda CU, Trotonda MP, Monzon M, Peris C, Amorena B, Lasa I, Penades JR, 2004. Role of Biofilm-Associated Protein Bap in the Pathogenesis of Bovine Staphylococcus aureus, Infection and Immunity, 72: 2177-2185.
• Cunha BA, 2005. Methicillin-Resistant Staphylococcus aureus: Clinical Manifestations and Antimicrobial Therapy, Clinical Microbiology and InfectiousDiseases, 1: 33-42.
• Çiftci A, Aksoy A, 2015. Antibiyotiklere Karşı Oluşan Direnç Mekanizmaları. Turkiye Klinikleri Journal of Veterinary Sciences, 1(2): 1-10.
• El-Azizi M, Rao S, Kanchanapoom T, Khardori N, 2005. In Vitro Activity of Vancomycin, Quinupristin/Dalfopristin and Linezolid Against İntact and Disrupted Biofilms of Staphylococci. Annals of Clinical Microbiology and Antimicrobials, Doi:10.1186/1476-0711-4-2.
• Erdoğmuş SF, Konak S, 2019. Determination of the Biofılm Production Capabilities of Staphylococcus spp. and the Effects of Some Antibıotics on These Isolates. Fresenius Environmental Bulletin, 7: 5253-5259.
• Grinholc M, Wegrzyn G, Kurlenda J, 2007. Evaluation of biofilm production and prevalence of the icaD gene in methicilin-resistant and methicilin susceptible Staphylococcus aureus strains isolated from patients with nosocomial infections and carriers. FEMS Immunology and Medical Microbiology, 50: 375-379.
• Gündoğan N, Ataol Ö, 2012. Et Örneklerinden İzole Edilen Staphylococcus aureus ve Koagülaz Negatif Stafilokok’ların Biyofilm Üretimi ve DNaz Aktivitelerinin Belirlenmesi, Türk Hijyen ve Deneysel Biyoloji Dergisi, 69(3): 135-142.
• Haaber J, Penades JR, Ingmer H, 2017. Transfer of Antibiotic Resistance in Staphylococcus aureus. Trends in Microbiology, 25(11): 893-905.
• İkiz S, Başaran B, Bingöl EB, Çetin Ö, Kaşıkcı G, Özgür NY, Uçmak M, Yılmaz Ö, Gündüz MC, Sabuncu A, 2013. Turkish Journal of Veterinary and Animal Sciences, 37 (5):569-574.
• Jefferson KK, 2004. What Drives Bacteria to Produce a Biyofilm? FEMS Microbiology Letters, 236: 163-173.
• Kloos WE, Bannerman TL, 2005. Update on Clinical Significance of Coagulase Negative Staphylococcus. Clinical Microbiology Reviews, 7: 117-140.
• Kaya F, 2016. Süt İşletmelerinden İzole Edilen Staphylococcus aureus Suşlarında ıcaA ve ıcaD Genleri ve Biyofilm Üretiminin Tespiti. Yüksek Lisans Tezi, Necmettin Erbakan Üniversitesi, Fen Bilimleri Enstitüsü, Konya.
• Kuyucuoğlu Y, Uçar M, 2002. Afyon Bölgesi Süt İneklerinde Subklinik ve Klinik Mastitislerin Görülme Oranları ve Etkili Antibiyotiklerin Tespiti. Veteriner Hekimler Mikrobioloji Dergisi, 1: 19-24.
• Lange CC, Brito MAVP, Reis DRL, Machado MA, Guimaraes AS, Azevedo ALS, Salles EB, Alvim MCT, Silva FS, Meurer IR, 2015. Species Level Identification of Staphylococci Isolated From Bovine Mastitis in Brazil Using Partial 16S rRNA Sequencing, Veterinary Microbiology, 176: 382-388.
• Mah TFC, O’Toole GA, 2001. Mechanisms of Biofilm Resistance to Antimicrobial Agents. Trends in Microbiology, 9:34-39.
• Mathur T, Singhal S, Khan S, Upadhyay D, Fatma T, Rattan A, 2005. Adverse Effect of Staphylococci Slime on in Vitro Activity of Glycopeptides, Japanese Journal of Infection Disease, 58: 353-357.
• Melchior MB, Fink-Gremmels J, Gaastra W, 2006. Comparative Assessment of the Antimicrobial Susceptibility of Staphylococcus aureus Isolates from Bovine Mastitis in Biofilm Versus Planktonic Culture. Journal of Veterinary Medicine B, 53: 326-332.
• Miao J, Liang Y, Chen L, Wang W, Wang J, Li B, Li L, Chen D, Xu Z, 2017. Formation and Development of Staphylococcus Biofilm: with Focus on Food Safety. Journal of Food Safety, Doi: 10.1111/jfs.12358.
• Nishimura S, Tsurumoto T, Yonekura A, Adachi K, Shindo H, 2006. Antimicrobial Susceptibility of Staphylococcus aureus and Staphylococcus epidermidis Biofilms İsolated From Infected Total Hip Arthroplasty Cases, Journal of Orthopaedic Science, 11: 46-50.
• Osman KM, Amer AM, Badr JM, Helmy NM, Elhelw RA, Orabi A, Bakry M, Saad ASA, 2016. Antimicrobial Resistance, Biofilm Formation and mecA Characterization of Methicillin Susceptible S. aureus and non-S. aureus of Beef Meat Origin in Egypt. Frontiers in Microbiology, Doi:10.3389/fmicb.2016.00222.
• Percival SL, Suleman L, Vuotto C, Donelli G, 2015. Healthcare-Associated Infections, Medical Devices and Biofilms: Risk, Tolerance and Control. Journal of Medical Microbiology, 64: 323-334.
• Pitkala A, Haveri M, Pyorala S, Myllys V, Honkanen-Buzalski T, 2004. Bovine Mastitis in Finland 2001-Prevalence, Distribution of Bacteria, and Antimicrobial Resistance. Journal of Dairy Science, 87: 2433-2441.
• Post JC, Stoodley P, Hall-Stoodley L, Ehrlich GD, 2004. The Role of Biofilms in Otolaryngologic Infections, Current Opinion of Otolaryngology Head and Neck Surgery, 12: 185-190.
• Rachid S, Ohlsen K, Witte W, Hacker J, Ziebuhr W, 2000. Effect of Subinhibitory Antibiotic Concentrations on Polysaccharide Intercellular Adhesin Expression in Biofilm Forming Staphylococcus epidermidis, Antimicrobial Agents and Chemotherapy, 44: 3357-3363.
• Resch A, Rosenstein R, Nerz C, Gotz F, 2005. Differential Gene Expression Profiling of Staphylococcus aureus Cultivated Under Biofilm and Planktonic Conditions. Applied and Environmental Microbiology, 71: 2663-2676.
• Sabuncuoğlu N, Çoban Ö, 2006. Mastitis Ekonomisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 1(1-2):1-5.
• Saidi R, Cantekin Z, Khelef D, Ergün Y, Solmaz H, Kaidi R, 2015. Antibiotic Susceptibility and Molecular Identification of Antibiotic Resistance Genes of Staphylococci Isolated from Bovine Mastitis in Algeria. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21 (4): 513-520.
• Savage VJ, Chopra I, O’Neill AJ, 2013. Staphylococcus aureus Biofilms Promote Horizontal Transfer of Antibiotic Resistance, Antimicrobial Agents and Chemotheraphy, 57: 1968-1970.
• Smeltzer MS, Gillaspy AF, 2000. Molecular pathogenesis of staphylococcal osteomyelitis. Poultry Science, 79: 1042-9.
• Stewart PS, 2002. Mechanisms of antibiotic resistance in bacterial biofilms. International Journal of Medical Microbiology, 292:107-113.
• Sümerkan B, Gökahmetoğlu S, 1998. MIC, MBC Testleri, Rutindeki Önemi ve Uygulamaları. Flora, 3(2): 91-95.
• Szczuka E, Kaznowski A, 2014. Antimicrobial Activity of Tigecycline Alone or İn Combination With Rifampin Against Staphylococcus epidermidis in Biofilm, Folia Microbiologica (Praha), 59: 283-288.
• Şahin R, 2007. Staphylococcus aureus Suşlarında Biyofilm Üretimi, Biyofilm Pozitif ve Negatif Suşların Genotipik ve Fenotipik Karakterlerinin Karşılaştırılması. Uzmanlık Tezi, Pamukkale Üniversitesi Tıp Fakültesi, Sağlık Bilimleri Enstitüsü, Pamukkale.
• Tetik T, Eryılmaz M, Akın A, 2010. Çeşitli Klinik Örneklerden İzole Edilen Stafilokok Suşlarında Slime Oluşumu ve Antibiyotik Direnci Arasındaki İlişkinin Araştırılması. Ankara Eczacılık Fakültesi Dergisi, 39 (3): 187-194.
• Uçan SU, Aslan E, 2002. Penicillin Resistance and Antimicrobial Susceptibilities of Coagulase Positive Staphylococci Isolated from Bovine Mastitic Milk Samples. Veteriner BiIimleri Dergisi, 18 (3): 19-22.
• Victoria J, Savage IC, O’Neill AJ, 2013. Staphylococcus aureus biofilms promote horizontal transfer of antibiotic resistance. Antimicrobial Agents and Chemotherapy, 57(4): 1968-1970.
• Wilcox MH, Kite P, Mills K, Sugden, S, 2001. In Situ Measurement of Linezolid and Vancomycin Concentrations in Intravascular Catheter-Associated Biofilm, Journal of Antimicrobial Chemotherapy, 47: 171-175.
• Yüksekdağ ZN, Baltacı N, 2013. Staphylococcus aureus Türlerinde Biyofilm ve Biyofilm Oluşumundan Sorumlu Genler, Türk Mikrobiyoloji Cemiyeti Dergisi,43(3): 77-83.
• Zecconi A, Scali F, 2013. Staphylococcus aureus Virulence Factors in Evasion from Innate Immune Defenses in Human and Animal Diseases. Immunology Letters, 150: 12-22.