Kanserli ve Sağlıklı Larenks Dokularında ICP-MS Yöntemi ile Eser Elementler Analizi

Yıl 2020, Cilt: 10 Sayı: 3, 1779 - 1785, 01.09.2020

Ömer Faruk Koçak , Mevlüt Albayrak , Fatma Betül Özgeriş

https://doi.org/10.21597/jist.681303

Öz

Eser elementler vücudun düzgün çalışması için gereklidir. Biyolojik element konsantrasyonundaki değişiklikler çok sayıda patolojik durumda gözlenmiştir. Bu çalışmanın amacı, larenks dokularındaki eser element konsantrasyonunun değerlendirilmesidir. Çalışmada larenks kanseri teşhisi konmuş 9 hastanın kanserli larenks dokuları ve aynı hastaların sağlıklı larekns dokuları kullanılmıştır. Larenks dokularındaki Al, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Ag, Cd, Sb ve Pb seviyeleri, Endüktif Eşleşmiş Plazma Kütle Spektrometresi (ICP-MS) kullanılarak belirlenmiştir. Sonuçlar kontrol grubu ile karşılaştırıldı ve larenks kanserli dokularda Cu seviyesinin sağlıklı dokulara göre anlamlı derecede (p <0.05) yüksek olduğu ve Al, Cr, Mn, As, Se, Ag ve Sb elementlerinin derişiminin ise sağlıklı dokulara göre anlamlı derecede düşük olduğu bulundu. Bu sonuçlar, larenks kanseri etyolojisinde eser elementlerin önemli rolleri olduğunu düşündürmektedir.

Anahtar Kelimeler

Larenks kanseri, eser elementler, indüktif eşleşmiş plazma - kütle spektrometresi, FFPE doku

Teşekkür

Atatürk Üniversitesi Tıp Fakültesi Tıbbi Patoloji Anabilim Dalı'na FFPE doku örneklerini sağladığı için içten teşekkürlerimizi sunarız.

Trace Elements Analysis in Cancer and Healthy Larynx Tissues by ICP-MS Method

Öz

Trace elements are necessary for the body to function properly. Changes in biological element concentration have been observed in a number of pathological conditions. The aim of this study is to evaluate the trace element concentration in the larynx tissues. Cancer laryngeal tissues and healthy larynx tissues of the same patients were used in the study. Al, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Ag, Cd, Sb and Pb levels in the larynx tissues were determined using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The results were compared with the control group and it was found that Cu level in tissues with laryngeal cancer was significantly higher (p <0.05) compared to healthy tissues, and the concentration of Al, Cr, Mn, As, Se, Ag, and Sb elements was significantly lower than healthy tissues. These results suggest that trace elements play an important role in the etiology of laryngeal cancer.

Anahtar Kelimeler

Laryngeal cancer, TRACE ELEMENTS, inductively coupled plasma - mass spectrometer, FFPE tissue

Kaynakça

• Bánfalvi G, 2011. Cellular effects of heavy metals. Springer Science & Business Media, pp. 3-28, New York-ABD
• Bleackley MR, MacGillivray RT, 2011. Transition metal homeostasis: from yeast to human disease. Biometals, 24(5): 785-809.
• Brewer GJ, 2005. Anticopper therapy against cancer and diseases of inflammation and fibrosis. Drug discovery today, 10(16): 1103-1109.
• Canene-Adams K, 2013. Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry. Methods in enzymology, 533: 225-233.
• Caner M, 2014. Icp-ms İle Demir Analizlerindeki Girişim Etkilerinin Gıda, Cam Ve Su Örneklerinde İncelenmesi, İstanbul Teknik Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi (Basılmış).
• Dobrowolski R, Klatka J, Brodnjak-Voncina D, Trojanowska A, Myśliwiec D, Ostrowski J, Remer M, 2014. Chemometric methods for studying the relationships between trace elements in laryngeal cancer and healthy tissues. Biological trace element research, 159(1-3): 107-114.
• Goodman V, Brewer, G, Merajver S, 2004. Copper deficiency as an anti-cancer strategy. Endocrine-related cancer, 11(2): 255-263.
• Golasik, M., Przybyłowicz, A., Woźniak, A., Herman, M., Gawęcki, W., Golusiński, W., Florek, E. (2015). Essential metals profile of the hair and nails of patients with laryngeal cancer. Journal of Trace Elements in Medicine and Biology, 31: 67-73.
• Kaba M, Pirincci N, Yuksel MB, Gecit I, Gunes M, Ozveren H,Eren H, Demir H, 2014. Serum levels of trace elements in patients with prostate cancer. Asian Pacific Journal of Cancer Prevention, 15(6): 2625-2629.
• Khlifi R, Olmedo P, Gil F, Hammami B, Chakroun A, Rebai A, Hamza-Chaffai A, 2013. Arsenic, cadmium, chromium and nickel in cancerous and healthy tissues from patients with head and neck cancer. Science of the total environment, 452: 58-67.
• Kohzadi S, Sheikhesmaili F, Rahehagh R, Parhizkar B, Ghaderi E, Loqmani H, Shahmoradi B, Mohammadi E, Maleki A, 2017. Evaluation of trace element concentration in cancerous and non-cancerous tissues of human stomach. Chemosphere, 184: 747-752.
• Çaloğlu M, Yürüt Çaloğlu V, Uzal C, Karagöl H, Tokatli F, Uygun K, 2005. Larenks Kanserli Olgularda Lokal Bölgesel Kontrol Sonuçlarımız ve Prognostik Faktörler: Trakya Üniversitesi Tıp Fakültesi Radyasyon Onkolojisi Anabilim Dalı Deneyimi. Turkish Journal of Oncology 20(1): 20-26
• Mikulewicz M, Chojnacka K, Gedrange T, Górecki H, 2013. Reference values of elements in human hair: a systematic review. Environmental toxicology and pharmacology, 36(3): 1077-1086.
• Niedzielska G, Caruk K, Pasternak K, 2000. Trace elements in neoplasm tissues of the larynx. Otolaryngologia polska= The Polish otolaryngology, 54: 200-202.
• Rębacz E, Baranowska-Bosiacka I, Chlubek D, 2010. The content of selected chemical elements in the hair of young men of the Bantu language group from Tanzania versus environmental and social conditioning. Biological trace element research, 137(3): 262-279.
• Reddy SB, Charles MJ, Raju GN, Vijayan V, Reddy BS, Kumar M R, Sundareswar B, 2003. Trace elemental analysis of carcinoma kidney and stomach by PIXE method. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 207(3): 345-355.
• Rostkowska-Nadolska B, Pośpiech L, 1999. The content of some trace elements in tissue of laryngeal squamous cell carcinoma. Otolaryngologia polska= The Polish otolaryngology, 53(3): 267-270.
• Samavarchi ST, Mahmoodzadeh HH, Yousefi T, Abolghasemi M, Qujeq D, Maniati M, Amani J, 2018. The crosstalk between trace elements with DNA damage response, repair, and oxidative stress in cancer. Journal of Cellular Biochemistry, 120: 1080-1105.
• Schwartz MK, 1975. Role of trace elements in cancer. Cancer research, 35: 3481-3487.
• Shang Z, Niu Y, Cai Q, Chen J, Tian J, Yeh S,Lai KP, Chang C, 2014. Human kallikrein 2 (KLK2) promotes prostate cancer cell growth via function as a modulator to promote the ARA70-enhanced androgen receptor transactivation. Tumor Biology, 35(3): 1881-1890.
• Yaman M, 2006. Comprehensive comparison of trace metal concentrations in cancerous and non-cancerous human tissues. Current medicinal chemistry, 13(21): 2513-2525.
• Yaman ME, 2018. İnvaziv Duktal Karsinom Tanisi Almiş Meme Dokusu Örneklerine Ait N-Bağli Glikozilasyon Yapilarinin Glikomiks Yaklaşimi İle Analizi Ve Biyobelirteç Potansiyellerinin Araştirilmasi, Atatürk Üniversitesi Sağlık Bilimleri Enstitüsü, Doktora Tezi (Basılmış).