An Investigation into Hematoprotective and Antioxidant Effects of Salvia virgata Jacq. Upon Cyclophosphamide-Induced Toxicity

Yıl 2022, Cilt: 12 Sayı: 2, 589 - 596, 01.06.2022

İlknur Kulcanay Şahin

https://doi.org/10.21597/jist.1007441

Öz

The present study aims to investigate hematoprotective and antioxidant effects of Salvia virgata (SV) on Cyclophosphamide (CP)-induced toxicity. The rats were divided into 6 groups of 7 members each. These were the Control group, CP Group (150 mg kg-1), 100 and 200 mg kg-1 SV Groups, and CP+100 and CP+200 mg kg-1 SV Groups. All the rats were sacrificed to harvest their blood and bone marrow samples the day after final injections. While some of the blood samples were used for leukocyte and platelet count, the others were used to determine the levels of total antioxidant capacity (TAC), malondialdehyde (MDA), total oxidant status (TOS), glutathione (GSH), and oxidative stress index (OSI). While a decrease was observed in the leukocyte, platelet and bone-marrow nucleated cell counts of the group given CP, apart from a decline in GSH and TAC levels, the MDA and TOC levels of the rats in this group showed an increase. In contrast, CP-induced oxidative stress and myelosuppression reversed in the combined SV Groups (CP+100 and 200 mg kg-1 of SV Groups), although the reversal in the latter group was of more significance. Our experimental results have shown that SV may exert antioxidant and cytoprotective effects upon CP-related peripheral blood and bone marrow toxicities.

Anahtar Kelimeler

Cyclophosphamide, hematotoxicity, salvia virgata, antioxidant, cytoprotective

Siklofosfamid Kaynaklı Toksisite Üzerine Salvia virgata Jacq'ın Hematoprotektif ve Antioksidan Etkilerinin Araştırılması

Öz

Bu çalışma, Salvia virgata'nın (SV) Siklofosfamid (CP) kaynaklı toksisite üzerindeki hematoprotektif ve antioksidan etkilerini araştırmayı amaçlamaktadır. Sıçanlar, her biri 7 üyeden oluşan 6 gruba ayrıldı. Bunlar Kontrol grubu, CP Grubu (150 mg kg-1), 100 ve 200 mg kg-1 SV Grupları ve CP+100 ve CP+200 mg kg-1 SV Gruplarıdır. Tüm sıçanlar, son enjeksiyonlardan sonraki gün kan ve kemik iliği numunelerini toplamak için kurban edildi. Kan örneklerinin bir kısmı lökosit ve trombosit sayımı için kullanılırken, diğerleri toplam antioksidan kapasite (TAC), malondialdehit (MDA), toplam oksidan durumu (TOS), glutatyon (GSH) ve oksidatif stres düzeylerini belirlemek için kullanıldı. indeks (OSI). CP verilen grupta lökosit, trombosit ve kemik iliği çekirdekli hücre sayılarında azalma görülürken, bu gruptaki ratların MDA ve TOC düzeylerinde GSH ve TAC düzeylerinde düşme dışında artış görüldü. Tersine, CP ile indüklenen oksidatif stres ve miyelosupresyon, kombine SV Gruplarında (CP+100 ve 200 mg kg-1 SV Gruplarında) tersine döndü, ancak ikinci gruptaki tersine dönüş daha önemliydi. Deneysel sonuçlarımız, SV'nin CP ile ilişkili periferik kan ve kemik iliği toksisiteleri üzerinde antioksidan ve sitoprotektif etkiler gösterebileceğini göstermiştir.

Anahtar Kelimeler

Siklofosfamid, hematotoksisite, salvia virgata, antioksidan, sitoprotektif

Kaynakça

• Ağgül AG, Gür F, Gülaboğlu M, 2021. Streptozotocin‐Induced Oxidative Stress in Rats: The Protective Role of Olive Leaf Extract. Bulletin of the Korean Chemical Society, 42(2), 180-187.
• Aycicek A, Erel O, Kocyigit A, 2005. Decreased total antioxidant capacity and increased oxidative stress in passive smoker infants and their mothers. Pediatrics International. 47, 635-639.
• Ayhanci A, Uyar R, Aral E, Kabadere S, Appak S, 2008. Protective effect of zinc on cyclophosphamide-induced hematoxicity and urotoxicity. Biological trace element research, 126(1), 186-193.
• Ayhanci A, Yaman S, Appak S, Gunes S, 2009. Hematoprotective effect of seleno-L-methionine on cyclophosphamide toxicity in rats. Drug and chemical toxicology, 32(4), 424-428.
• Ayhanci A, Heybeli N, Sahin İK, Cengiz M, 2019. Myelosuppression and Oxidative Stress Induced by Cyclophosphamide in Rats: The Protective Role of Selenium. Adıyaman University Journal of Science, 9(2), 252-265.
• Buyukokuroglu ME, Taysi S, Buyukavci M, Memisogullari R, Ozabacigil F, Bakan E, 2007. Dantrolene: in doxorubicin toxicity. Asian Journal of Chemistry, 19(5), 4035.
• Çadirci E, Süleyman H, Gürbüz P, Uz Ayşe, Güvenalp Z, Dermirezer LÖ, 2012. Anti-inflammatory effects of different extracts from three Salvia species. Turkish Journal of Biology, 36(1), 59-64.
• Cengiz M, Ayhanci A, Kutlu HM, Musmul, A, 2016. Potential therapeutic effects of silymarin and silymarin-loaded solid lipidnanoparticles on experimental kidney damage in BALB/c mice: biochemical and histopathological evaluation. Turkish Journal of Biology, 40(4), 807-814.
• Cengiz M, 2018. Hematoprotective effect of boron on cyclophosphamide toxicity in rats. Cellular and Molecular Biology. 64, 62-65.
• Cengiz M, Yeşildağ Ö, Ayhanci A, 2018. Siklofosfamid Nedenli Hematoksisite Üzerine Karvakrolün Sitoprotektif Etkileri. Türkiye Tarımsal Araştırmalar Dergisi. 5, 125-130.
• Cengiz M, Yildiz SC, Demir C, Şahin İK, Teksoy Ö, Ayhanci A, 2019. Hepato-preventive and anti-apoptotic role of boric acid against liver injury induced by cyclophosphamide. Journal of Trace Elements in Medicine and Biology, 53, 1-7.
• Cengiz M, Kutlu HM, Peker Cengiz B, Ayhancı A, 2020. Escin attenuates oxidative damage, apoptosis and lipid peroxidation in a model of cyclophosphamide-induced liver damage. Drug and Chemical Toxicology, 1-8.
• Erel O, 2005. A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry. 38, 1103-1111.
• Fraiser L, Kanekal S, Kehrer J, 1991. Cyclophosphamide toxicity: characterizing and avoiding the problem. Drugs. 42, 781-5.
• Furtado RA, De Araujo FRR, Resende FA, Cunha WR, Tavares DC, 2010. Protective effect of rosmarinic acid on V79 cells evaluated by the micronucleus and comet assays. Journal of Applied Toxicology: An International Journal, 30(3), 254-259. Gür F, Cengiz M, Kutlu HM, Cengiz BP, & Ayhancı, A, 2021. Molecular docking analyses of Escin as regards cyclophosphamide-induced cardiotoxicity: In vivo and in Silico studies. Toxicology and Applied Pharmacology, 411, 115386.
• Kawabata TT, Chapman MY, Dong-Hyun K, Stevens WD, Holsapple MP, 1990. Mechanisms of in vitro immunosuppression by hepatocyte-generated cyclophosphamide metabolites and 4-hydroperoxycyclophosphamide. Biochemical pharmacology, 40(5), 927-935.
• Kumar K, Kuttan R, 2005. Chemoprotective activity of an extract of Phyllanthus amarus against cyclophosphamide induced toxicity in mice. Phytomedicine. 12, 494-500.
• Moore FR, Urda GA, Krishna G, Theiss JC, 1995. An in vivo/in vitro method for assessing micronucleus and chromosome aberration induction in rat bone marrow and spleen 1. Studies with cyclophosphamide. Mutation Research/Environmental Mutagenesis and Related Subjects, 335(2), 191-199.
• Schuurman HJ, Smith HT, Cozzi E, 2005. Tolerability of cyclosphosphamide and methotrexate induction immunosuppression in nonhuman primates. Toxicology. 213, 1-12.
• Senthilkumar S, Yogeeta SK, Subashini R, Devaki T, 2006. Attenuation of cyclophosphamide induced toxicity by squalene in experimental rats. Chemico-biological interactions, 160(3), 252-260.
• Taysi S, Memisogullari R, Koc M, Yazici AT, Aslankurt M, Gumustekin K, Tahsin Ozder H, 2008. Melatonin reduces oxidative stress in the rat lens due to radiation-induced oxidative injury. International journal of radiation biology, 84(10), 803-808.
• Teksoy O, Sahinturk V, Cengiz M, İnal B, Ayhancı A, 2020. The Protective Effects of Silymarin on Thioacetamide-Induced Liver Damage: Measurement of miR-122, miR-192, and miR-194 Levels. Applied biochemistry and biotechnology, 191(2), 528-539.
• Tepe B, 2008. Antioxidant potentials and rosmarinic acid levels of the methanolic extracts of Salvia virgata (Jacq), Salvia staminea (Montbret & Aucher ex Bentham) and Salvia verbenaca (L.) from Turkey. Bioresource Technology. 99, 1584-1588.
• Trasler JM, Hales BF, Robaire B, 1987. A time-course study of chronic paternal cyclophosphamide treatment in rats: effects on pregnancy outcome and the male reproductive and hematologic systems. Biology of reproduction. 37, 317-326.
• Ucuncu H, Aktan B, Ozabacigil F, Buyukokuroglu ME, Akyuz M, Altas E, 2004. Protective effect of melatonin on experimental otitis media with effusion in guinea pigs. Annals of Clinical & Laboratory Science, 34(3), 341-346.
• Yoruk O, Ucuncu H, Sutbeyaz Y, Aktan B, Gur FO, 2009. The effects of methylprednisolone and cefazolin sodium on antioxidant status in experimentally induced maxillary sinusitis. Acta oto-laryngologica, 129(10), 1101-1105.