Determination Effects of Rheum ribes L. against High Calorie Diet-induced Obesity: Investigation of Changes in Immunologic and Neurologic Enzymes Activities

Yıl 2022, Cilt: 12 Sayı: 4, 1975 - 1984, 01.12.2022

Bedia Bati

https://doi.org/10.21597/jist.1124795

Öz

This study was carried out to investigate the immunotoxic and neurotoxic effects of Rheum ribes L. (Rr) plant extract on liver, brain and heart tissues in rats with an experimental obesity model. Wistar albino male rats were used in the study. In this study, 4 groups were formed, one of which was the control group, and each group consisted of 6 rats. The groups were the ''control (CG)'', ''high calorie diet (HCD) control (HCDG)'', 'HCD + Rr (200 mg/kg) (HCDRHE1)'' and ''HCD + Rr (400 mg/kg) (HCDRHE2)''. At the end of the study, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), adenosineaminase (ADA) and myeloperoxidase (MPO) biomarkers were evaluated in tissues. According to the study findings, the ADA and MPO activity levels of the obese group increased significantly compared to the control group, and these enzyme levels in the groups administered Rr plant extract approached the control group levels. On the other hand, the AChE and BChE activity levels of the obese group decreased compared to the control group, and there was a statistically significant increase in the groups administered plant extracts compared to the control group. In conclusion, Rr plant root extract suppresses neurotoxicity and immunotoxicity in rats with obesity induced by a high-calorie diet (HCD).

Anahtar Kelimeler

Acetylcholinesterase, adenosineaminase, butyrylcholinesterase, myeloperoxidase, obesity, Rheum ribes

Kaynakça

• Al-Kassab AS, Vijayakumar E, 1995. Profile of serum cholinesterase in systemic sepsis syndrome (septic shock) in intensive care unit patients. Clinical Chemistry and Laboratory Medicine, 33 (1):11–14.
• Almalki DA, Alghamdi SA, Al-Attar AM, 2019. Comparative study on the influence of some medicinal plants on diabetes induced by streptozotocin in male rats. BioMed Research International.
• Altunkaynak ME, Ozbek E, Altunkaynak BZ, Can I, Unal D, Unal B, 2008. The effects of high-fat diet on the renal structure and morphometric parametric of kidneys in rats. Journal Anat, 212: 845-852.
• Amiri N, Shafaghat A, Salimi F, 2015. Screening of the Essential Oil, Hexane Extract, Chemical Composition, Antioxidant Activity, and Antimicrobial Acitivity of the Flower Rheum ribes L. from Iran. Journal of Essential Oil Bearing Plants, 18 (5): 1108-1115.
• Antonioli L, Colucci R, La Motta C, Tuccori M, Awwad O, Da Settimo F, Fornai M, 2012. Adenosine deaminase in the modulation of immune system and its potential as a novel target for treatment of inflammatory disorders. Current drug targets, 13: (6) 842-862.
• Bati B, Celik I, Turan A, Eray N, Alkan EE, Zirek AK, 2020. Effect of isgin (Rheum ribes L.) on biochemical parameters, antioxidant activity and DNA damage in rats with obesity induced with high-calorie diet. Archives of Physiology and Biochemistry, 1-9.
• Bati B, Celik I, Vuran Eray N, Turan A, Alkan EE, Zirek AK, 2021. Effects of Gundelia tournefortii L. on biochemical parameters, antioxidant activities and DNA damage in a rat model of experimental obesity. Brazilian Journal of Biology, 83.
• Bitzinger DI, Gruber M, Tummler S, Malsy M, Seyfried T, Weber F, ... Zausig YA, 2019. In vivo effects of neostigmine and physostigmine on neutrophil functions and evaluation of acetylcholinesterase and butyrylcholinesterase as inflammatory markers during experimental sepsis in rats. Mediators of Inflammation,
• Bradley PP, Priebat DA, Christensen RD, Rothstein G, 1982. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. Journal of investigative dermatology, 78 (3): 206-209.
• Caglayan C, Taslimi P, Demir Y, Kücükler S, Kandemir FM, Gulcin I, 2019. The effects of zingerone against vancomycin‐induced lung, liver, kidney and testis toxicity in rats: The behavior of some metabolic enzymes. Journal of biochemical and molecular toxicology, 33(10), e22381.
• Chiarla C, Giovannini I, Giuliante F, Vellone M, Ardito F, Nuzzo G, 2011. Plasma cholinesterase correlations in acute surgical and critical illness. Minerva chirurgica, 66 (4): 323-327.
• Dalar A, Konczak I, 2013. Phenolic contents, antioxidant capacities and inhibitory activities against key metabolic syndrome relevant enzymes of herbal teas from Eastern Anatolia. Industrial Crops & Products 44: 383-390.
• Das UN, 2007. Acetylcholinesterase and butyrylcholinesterase as possible markers of low-grade systemic inflammation. Medical science monitor: international medical journal of experimental and clinical research, 13 (12): RA214-21.
• de Lima R, Guex CG, da Silva ARH, Lhamas CL, dos Santos Moreira KL, Casoti R, ... Manfron MP, 2018. Acute and subacute toxicity and chemical constituents of the hydroethanolic extract of Verbena litoralis Kunth. Journal of ethnopharmacology, 224: 76-84.
• Dogan A, 2015. The healing effects of some plant extracts on experimentally induced diabetic rats. (PhD Thesis) Van Yuzuncu Yil Universty, Graduate School of Natural and Applied Sciences, Turkey.
• Dogan A, Donmez F, Battal A, Aslan A, Anuk OO, 2020. Investigation of neurotoxic and immunotoxic effects of the chinar (Platanus orientalis L.) tree leaf infusion against ethanol toxicity in rats. Journal of the Institute of Science and Technology, 10 (2): 778-787.
• Ellman GL, Courtney KD, Andres Jr V, Featherstone RM, 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7 (2): 88-95.
• Giusti G, Bergmeyer HU, 1974. Handbook of enzymatic methods of analysis. New York Academic Press No:2, 1092–1099. New York-USA.
• Gok M, 2021. Interaction of human serum butyrylcholinesterase with lipid metabolism of liver HepG2 cells. Hacettepe University Institute of Health Sciences, Biochemistry Program, Ankara, PhD Thesis.
• Golombek DA, Casiraghi LP, Agostino PV, Paladino N, Duhart JM, Plano SA, Chiesa JJ, 2013. The times they’re a-changing: Effects of circadian desynchronization on physiology and disease. Journal of Physiology-Paris, 107 (4): 310-322.
• Gulcin I, Scozzafava A, Supuran CT, Koksal Z, Turkan F, Cetinkaya S, ... Alwasel SH, 2016. Rosmarinic acid inhibits some metabolic enzymes including glutathione S-transferase, lactoperoxidase, acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase isoenzymes. Journal of enzyme inhibition and medicinal chemistry, 31 (6): 1698-1702.
• Hussaini ZS, Askndari H, Alami K, Mousavi SY, 2021. Effect of Rheum Ribes and Urtica Dioica on type 2 diabetic rats. International Journal of Pharmaceutical and Phytopharmacological Research, 11 (1): 63-69.
• Jadhav AA, Jain A, 2012. Elevated adenosine deaminase activity in over weight and obese Indian subjects. Archives of physiology and Biochemistry, 118 (1): 1-5.
• Kalaz EB, Aydın AF, Dogan-Ekici I, Coban J, Dogru-Abbasoglu S, Uysal M, 2016. Protective effects of carnosine alone and together with alpha-tocopherol on lipopolysaccharide (LPS) plus ethanol-induced liver injury. Environmental Toxicology and Pharmacology, 42: 23-29.
• Keser S, Keser F, Karatepe M, Kaygili O, Tekin S, Turkoglu I, ...Sandal S, 2020. Bioactive contents, In vitro antiradical, antimicrobial and cytotoxic properties of rhubarb (Rheum ribes L.) extracts. Natural product research, 34 (23): 3353-3357.
• Kurdoglu Z, Ozkol H, Kurdoglu M, Kamaci M, 2012. Evaluation of the relationship between adenosine deaminase, myeloperoxidase, cholinesterase, preeclampsia severity, and neonatal outcomes. Clinical and experimental hypertension, 34 (7): 493-497.
• Kurtul N, Akarsu E, Aktaran S, 2006. The relationship between serum total sialic acid levels and adenosine deaminase activity in obesity. Saudi medical journal, 27 (2): 170.
• Lajter I, Zupkó I, Molnár J, Jakab G, Balogh L, Vasas A, Hohmann J, 2013. Antiproliferative activity of Polygonaceae species from the Carpathian Basin against human cancer cell lines. Phytotherapy Research, 27 (1): 77-85.
• Lasker S, Rahman MM, Parvez F, Zamila M, Miah P, Nahar K, ... Alam MA, 2019. High-fat diet-induced metabolic syndrome and oxidative stress in obese rats are ameliorated by yogurt supplementation. Scientific Reports, 9 (1): 1-15.
• Lima JK, Leite N, Turek LV, Souza RLR, da Silva Timossi L, Osiecki ACV, ...Furtado-Alle L, 2013. 1914G variant of BCHE gene associated with enzyme activity, obesity and triglyceride levels. Gene, 532 (1): 24-26.
• Lu N, Sui Y, Tian R, Peng YY, 2018. Inhibitiveeffects of quercetin on myeloperoxidase-dependent hypochlorousacid formation and vascular endothelial injury. Journal of Agricultural and Food Chemistry, 66(19): 4933-4940.
• Macit MS, Köksal E, 2020. The Role of Coffee Consumption in the Treatment of Obesity. Journal of Traditional Medical Complementary Therapies, 3 (1): 119-127.
• Manzoni AG, Passos DF, da Silva JL, Bernardes VM, Bremm JM, Jantsch MH, ... Leal DB, 2019. Rutin and curcumin reduce inflammation, triglyceride levels and ADA activity in serum and immune cells in a model of hyperlipidemia. Blood Cells, Molecules, and Diseases, 76, 13-21.
• Meral R, 2011. The effects of natural compounds having functional properties on dough rheologhy and bread. Van Yuzuncu Yil University, Department of Food Engineering, PhD Thesis. Müller A, Olbert M, Heymann A, Zahn PK, Plaschke K, von Dossow V, ... Spies CD, 2019. Relevance of peripheral cholinesterase activity on postoperative delirium in adult surgical patients (CESARO): a prospective observational cohort study. European Journal of Anaesthesiology, 36 (2):114-122.
• Nussbaum C, Klinke A, Adam M, Baldus S, Sperandio M, 2013. Myeloperoxidase: a leukocyte-derived protagonist of inflammation and cardiovascular disease. Antioxidants & redox signaling, 18 (6): 692-713.
• OECD, 2008. Guidelines for the testing of chemicals. Acute oral toxicity- Up an down procedure. (OECD-425).
• Olson JR, 2018. Biomarkers of chlorpyrifos and profenofos exposure and effect in adult and adolescent egyptian cotton field workers.
• Ozkol H, Bulut G, Balahoroglu R, Tuluce Y, Ozkol HU, 2017. Protective effects of selenium, N-acetylcysteine and vitamin E against acute ethanol intoxication in rats. Biologicaltrace element research, 175 (1):177-185.
• Ozkol H, Musa D, Tuluce Y, Koyuncu I, 2012. Ameliorative influence of Urtica dioica L against cisplatin-induced toxicity in mice bearing Ehrlich ascites carcinoma. Drug and chemical toxicology, 35 (3):251-257.
• Ozok N, Celik I, 2019. Investigation of the Effects of Linden (Tilia platyphyllos Scop) Infusion on Immune System in Rats. BEU Journal of Science, 8 (3): 852-858.
• Ozturk M, Aydogmus-Ozturk F, Duru ME, Topcu G, 2007. Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food chemistry, 103 (2): 623-630.
• Petrie JR, Guzik TJ, Touyz RM, 2018. Diabetes, hypertension, and cardiovascular disease: clinical insights and vascular mechanisms. Canadian Journal of Cardiology, 34 (5): 575-584.
• R Core Team, 2022. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/
• Rahman MM, Shahab NB, Miah P, Rahaman MM, Kabir AU, Subhan N, ...Alam MA, 2021. Polyphenol-rich leaf of Aphanamixis polystachya averts liver inflammation, fibrogenesis and oxidative stress in ovariectomized Long-Evans rats. Biomedicine & Pharmacotherapy, 138: 111530.
• Shenhar-Tsarfaty S, Sherf-Dagan S, Berman G, Webb M, Raziel A, Keidar A, ...Zelber-Sagi S, 2019. Obesity-related acetylcholinesterase elevation is reversed following laparoscopic sleeve gastrectomy. International Journal of Obesity, 43 (2): 297-305.
• Spiegel K, Tasali E, Leproult R, Van Cauter E, 2009. Effects of poor and short sleep on glucose metabolism and obesity risk. Nature Reviews Endocrinology, 5 (5): 253-261.
• Takcı HAMT, Turkmen FU, Günes M, Bakırhan P, 2021. Antibacterial and Antioxidant Activities of Rheum ribes extracts. The Black Sea Journal of Sciences, 11 (1): 104-117.
• Tamura R, Ohta H, Satoh Y, Nonoyama S, Nishida Y, Nibuya M, 2016. Neuroprotective effects of adenosine deaminase in the striatum. Journal of Cerebral Blood Flow & Metabolism, 36(4): 709- 720.
• Tung YT, Chiang PC, Chen YL, Chien YW, 2020. Effects of melatonin on lipid metabolism and circulating irisin in sprague-dawley rats with diet-induced obesity. Molecules, 25 (15): 3329.
• Turkan F, 2021. Investigation of the toxicological and inhibitory effects of some benzimidazole agents on acetylcholinesterase and butyrylcholinesterase enzymes. Archives of Physiology and Biochemistry, 127(2), 97-101.
• Ugbaja RN, Ugwor EI, Dosumu OA, James AS, Thomas FC, Ezenandu EO, Kosoko AM, 2021. Lycopene abrogates obesity-provoked hyperactivity of neurosignalling enzymes, oxidative stress and hypothalamic inflammation in female Wistar rats. Neurochemistry International, 149: 105125.
• Vaisi‐Raygani A, Rahimi Z, Entezami H, Kharrazi H, Bahrhemand F, Tavilani H, ... Pourmotabbed T, 2008. Butyrylcholinesterase K variants increase the risk of coronary artery disease in the population of western Iran. Scandinavian journal of clinical and laboratory investigation, 68 (2):123-129.
• Wang HN, Xiang JZ, Qi Z, Du M, 2022. Plant extracts in prevention of obesity. Critical Reviews in Food Science and Nutrition, 62(8), 2221-2234.
• WHO, 2020. World Health Organization. Obesity and Overweight. Available online: https://www.who.int/news-room/ fact-sheets/detail/obesity-and-overweight (accessed on 20 May 2020).
• Yang YQ, Lu RS, Gao FF, Zhang J, Liu FL, 2020. Berberine induces lipolysis in porcine adipocytes by activating the AMP-activated protein kinase pathway. Molecular Medicine Reports 21 (6):2603–14. doi: 10.3892/mmr.2020.11070.