Determination of chemical compositions and antifungal activities of laurel and fennel essential oils against fungal disease agents of cypress seedlings

Yıl 2020, Cilt: 17 Sayı: 2, 264 - 275, 27.05.2020

Merve Kara , Soner Soylu , Musa Türkmen , Alpaslan Kaya

https://doi.org/10.33462/jotaf.663452

Cited By: 16

Öz

Fusarium oxysporum and Pestalotiopsis funerea are the most common fungal disease agents of conifer seedlings causing root rot and shoot or tip blight diseases. In this study, chemical compositions and antifungal activities of essential oils of fennel (Foeniculum vulgare Mill.) and laurel (Laurus nobilis L.) were determined against root rot and wilt disease agents F. oxysporum and P. funerea in vitro conditions. Chemical compositions of essential oils were determined by using GC-MS analysis. Antifungal volatile phase effects of essential oils were determined on inhibition of mycelial growth in vitro conditions by using different concentrations. The effect of most effective concentrations of essential oils on the morphology of fungal hypha was also determined by using light microscope. GC-MS analysis of essential oils of laurel and fennel plants revealed that eucalyptol (46.97%) and α-terpinyl acetate (19.82%) were major components of laurel; trans-anethole (82.44%) and limonene (5.18%) were major components of fennel essential oils. Volatile phase effects of fennel and laurel essential oils were found to completely inhibit mycelial growth of F. oxysporum at 30.0 and 50.0 µl petri-1 concentrations, respectively. Complete growth inhibition of P. funerea by essential oil of fennel and laurel were observed at relatively lower concentrations (20.0 and 25.0 µl petri-1 concentrations, respectively). Light microscopic observations on hyphae, exposed to volatile phase of the most efficient concentrations of essential oil, revealed considerable structural deformations such as cytoplasmic coagulation, vacuolations and protoplast leakage. In conclusion, our results suggest that essential oils have the potential for use in control of fungal diseases of conifer plants.

Anahtar Kelimeler

Fennel, laurel, essential oil

Teşekkür

Bu çalışma "3rd Mediterranean Symposium on Medicinal and Aromatic Plants (MESMAP-3) Merit Park Hotel in Girne (Kyrenia), Turkish Republic of Northern Cyprus (TRNC) April 13-16, 2017" bildiri kitapçığında ÖZET olarak sunulmuştur

Servi fidanlarında sorun olan fungal hastalık etmenlerine karşı defne ve rezene uçucu yağlarının kimyasal bileşenleri ve antifungal etkinliğinin belirlenmesi

Öz

Fusarium oxysporum ve Pestalotiopsis funerea, kozalaklı bitki türlerinde kök çürüklüğü ve sürgün ya da uç yanıklığı hastalıklarına sebep olan en yaygın fungal hastalıklardır. Bu çalışmada, rezene (Foeniculum vulgare Mill.) ve defne (Laurus nobilis L.) bitkilerinden elde edilen uçucu yağların kimyasal bileşimleri ve F. oxysporum ve P. funerea’ya karşı antifungal etkileri in vitro koşullarda belirlenmiştir. Uçucu yağların kimyasal bileşimleri, GC-MS analizi kullanarak belirlenmiştir. Uçucu yağların buhar fazında misel gelişimini engellemesi üzerine antifungal etkileri in vitro koşullarda farklı konsantrasyonlar kullanarak belirlenmiştir. Işık mikroskobu kullanarak uçucu yağların en etkili konsantrasyonlarının fungal hiflerin morfolojisine etkileri de belirlenmiştir. Uçucu yağlarının GC-MS analizleri, eucalyptol (%46.97) ve α-terpinyl acetate (%19.82)’ın defnenin, trans-anethole (%82.44) ve limonene (%5.18) ise rezene uçucu yağının ana bileşenleri olduğunu göstermiştir. Rezene ve defne uçucu yağlarının buhar fazında F. oxysporum’un miselyal gelişimini tamamen engelleyen konsantrasyonları sırasıyla 30.0 ve 50.0 µl petri-1 olarak belirlenmiştir. Rezene ve defne uçucu yağlarının P. funerea’nın gelişimini tamamen engelleyen konsantrasyonların nispeten daha düşük olduğu gözlenmiştir (sırasıyla 20.0 ve 25.0 µl petri-1 konsantrasyonlarında). Uçucu yağların buhar fazında en etkili konsantrasyonuna maruz kalan hifler üzerinde yapılan ışık mikroskobu gözlemleri, sitoplazmik pıhtılaşma, vakuolleşme ve protoplazma akıntısı gibi önemli yapısal deformasyonlar ortaya çıkarmıştır. Sonuç olarak bulgularımız uçucu yağların kozalaklı bitkilerin fungal hastalıklarının kontrolünde kullanım potansiyeli bulunduğunu ortaya koymaktadır.

Anahtar Kelimeler

Rezene, defne, uçucu yağ

Kaynakça

• Ahmed, A.F., Shi, M.J., Liu, C.Y., Kang, W.Y. (2019). Comparative analysis of antioxidant activities of essential oils and extracts of fennel (Foeniculum vulgare Mill.) seeds from Egypt and China. Food Science and Human Wellness 8: 67-72.
• Bajo, J., Santamarıa, O., Diez, J.J. (2008). Cultural characteristics and pathogenicity of Pestalotiopsis funerea on Cupressus arizonica. Forest Pathology 38: 263–274. Bakkali, F., Averbeck, S., Averbeck, D., Waomar, M. (2008). Biological effects of essential oils-A review. Food and Chemical Toxicology 46: 446-475.
• Bayar, Y., Onaran, A., Yılar, M., Gul, F. (2018). Determination of the essential oil composition and the antifungal activities of bilberry (Vaccinium myrtillus L.) and bay Laurel (Laurus nobilis L.). Journal of Essential Oil Bearing Plants 21: 548-555.
• Bibiano, H.D., Saber, M.L. (2017). Mycelial growth inhibition of plant pathogenic fungi by extracts. Revista Agrogeoambiental 9: 61-71.
• Bouzouita, N., Nafti, A., Chaabouni, M.M., Lognay, G.C., Marlier, M., Zghoulli, S., Thonart, P.H. (2001). Chemical composition of Laurus nobilis oil from Tunisia. Journal of Essential Oil Research 13: 116-117.
• Burt, S. (2004). Essential oils: Their antibacterial properties and potential applications in foods – A review. International Journal of Food Microbiology 94: 223–253.
• Chahal, K.K., Kaur, M., Bhardwaj, U., Singla, N., Kaur, A. (2017). A review on chemistry and biological activities of Laurus nobilis L. essential oil. Journal of Pharmacognosy and Phytochemistry 6: 1153-1161.
• Chen, T.W., Lu, J., Kang, B.B., Lin, M.S., Ding, L.J., Zhang, L.Y., Chen, G.Y., Chen, S.J., Lin, H.T. (2018). Antifungal activity and action mechanism of ginger oleoresin against Pestalotiopsis microspora isolated from Chinese olive fruits. Frontiers in Microbiology 9: 2583.
• Choudhary, D., Kala, S.P., Todaria, N.P., Dascupta, S., Kinhal, G., Kollmair, M. (2013). Essential oil from bay leaves in India and Nepal: An analysis for quality oriented value chain development. International Journal of Medicinal and Aromatic Plants 3: 11-17.
• Demir, V., Gunhan, T., Yagcioglu, A.K., Degirmencioglu, A. (2004). Mathematical modelling and the determination of some quality parameters of air-dried bay leaves. Biosystems Engineering 88: 325-335. Elkıran, O., Akbaba, E., Bagcı, E. (2018). Constituents of essential oils from leaves and seeds of Laurus nobilis L.: A Chemotaxonomic approach. Bangladesh Journal of Botany 47(4): 893-901.
• Gordon, T.R., Swett, C.L., Wingfield, M.J. (2015). Management of Fusarium diseases affecting conifers. Crop Protection 73: 28-39.
• Gross, M., Friedman, J., Dudai, N., Larkov, O., Cohen, Y., Bar, E., Ravid, U., Putievsky, E., Lewinsohn, E. (2002). Biosynthesis of estragole and t-anethole in bitter fennel (Foeniculum vulgare Mill. var. vulgare) chemotypes. Changes in SAM: phenylpropene O-methyltransferase activities during development. Plant Science 163: 1047-1053.
• Ho, C.L., Hua, K.F., Hsu, K.P., Wang, E.I.C., Su, Y.C. (2012). Composition and antipathogenic activities of the twig essential oil of Chamaecyparis formosensis from Taiwan. Natural Product Communications 7: 933-936.
• Isman, B.M. (2000). Plant essential oils for pest and disease management. Crop Protection 19: 603-608.
• Kalleli, F., Rebey, I.B., Wannes, W.A., Boughalleb, F., Hammam, M., Tounsi, M.S., M'hamdi, M. (2019). Chemical composition and antioxidant potential of essential oil and methanol extract from Tunisian and French fennel (Foeniculum vulgare Mill.) seeds. Journal of Food Biochemistry 43: 12935.
• Kan, Y., Kartal, M., Aslan, S., Yıldırım, N. (2006). Farklı koşullarda yetiştirilen rezene meyvelerinin uçucu yağ bileşenleri. Journal of Faculty of Pharmacy of Ankara University 35(2): 95-101.
• Kurt, S., Uysal, A., Kara, M., Soylu, S., Soylu, E.M. (2017). Natural infection of potato by Sclerotinia sclerotiorum causing stem rot disease in Turkey. Australasian Plant Disease Notes 12: 39.
• Mimica-Dukic, N., Kujundzic, S., Sokovic, M., Couladis, M. (2003). Essential oil composition and antifungal activity of Foeniculum vulgare Mill. obtained by different distillation conditions. Phytotherapy Research 17: 368–371.
• Nazzaro, F., Fratianni, F., Coppola, R., Feo, V.D. (2017). Essential oils and antifungal activity. Pharmaceuticals 10: 86. Ozcan, M.M., Chalchat, J.C., Arslan, D., Ates, A., Unver, A. (2006). Comparative essential oil composition and antifungal effect of bitter fennel (Foeniculum vulgare ssp. piperitum) fruit oils obtained during different vegetation. Journal of Medicinal Food 9: 552-561.
• Ozcan, B., Esen, M., Sangun, M.K., Coleri, A., Caliskan, M. (2010). Effective antibacterial and antioxidant properties of methanolic extract of Laurus nobilis seed oil. Journal of Environmental Biology 31: 637-641.
• Ozel, A., Kosar, I., Demirbilek, T., Erden, K. (2019). Changes in yields and volatile oil composition of fennel (Foeniculum vulgare Mill.) in high plant populations. Italian Journal of Agronomy 14: 147-152.
• Park, J.Y., Kim, S.H., Kim, N.H., Lee, S.W., Jeun, Y.C., Hong, J.K. (2017). Differential inhibitory activities of four plant essential oils on in vitro growth of Fusarium oxysporum f. sp fragariae causing Fusarium wilt in strawberry plants. Plant Pathology Journal 33: 582-588.
• Peixoto, L.R., Rosalen, P.L., Ferreira, G.L., Freires, I.A., de Carvalho, F.G., Castellano, L.R., de Castro, R.D. (2017). Antifungal activity, mode of action and anti-biofilm effects of Laurus nobilis Linnaeus essential oil against Candida spp. Archives Oral Biology 73: 179-185.
• Perez, R.A., Navarro, T., de Lorenzo, C. (2007). HS-SPME analysis of the volatile compounds from spices as a source of flavour in 'Campo Real' table olive preparations. Flavour and Fragrance Journal 22: 265-273.
• Pirbalouti, A.G., Firoznezhad, M., Craker, L., Akbarzadeh, M.A. (2013). Essential oil compositions, antibacterial and antioxidant activities of various populations of Artemisia chamaemelifolia at two phenological stages. Revista Brasileira de Farmacognosia 23: 861-869.
• Pinheiro, L.S., Filho, A.A.O., Guerra, F.Q.S., Menezes, C.P., Santos, S.G., Sousa, J.P., Dantas, T.B., Lima, E.O. (2017). Antifungal activity of the essential oil isolated from Laurus nobilis L. against Cryptococcus neoformans strains. Journal of Applied Pharmaceutical Science 7: 115-118.
• Rasooli, I., Rezaei, M.B., Allameh, A. (2006). Growth inhibition and morphological alterations of Aspergillus niger by essential oils from Thymus eriocalyx and Thymus x-porlock. Food Control 17: 359-364.
• Rather, M.A., Dar, B.A., Sofi, N.S., Bhat, B.A., Qurishi, M.A. (2016). Foeniculum vulgare: A comprehensive review of its traditional use, phytochemistry, pharmacology, and safety. Arabian Journal of Chemistry 9: 1574-1583.
• Sangun, M.K., Aydın, E., Timur, M., Karadeniz, H., Caliskan, M., Ozkan, A. (2007). Comparison of chemical composition of the essential oil of Laurus nobilis L. leaves and fruits from different regions of Hatay, Turkey. Journal of Environmental Biology 28: 731-733.
• Sinclair, W.A., Lyon, H.H., Johnson, W.T. (1993). Diseases of Trees and Shrubs. New York: Cornell University Press.
• Soylu, S., Yigitbas, H., Soylu, E.M., Kurt, S. (2007). Antifungal effects of essential oils from oregano and fennel on Sclerotinia sclerotiorum. Journal of Applied Microbiology 103(4): 1021-1030.
• Soylu, E.M., Kurt, S., Soylu, S. (2010). In vitro and in vivo antifungal activity of essential oils of various plants against tomato grey mould disease agent Botrytis cinerea. International Journal of Food Microbiology 143(3): 183-189.
• Soylu, E.M., Incekara, R. (2017). Biofungicidal activities of plant essential oils against cucumber root and stem rot disease caused by Fusarium oxysporum f. sp radicis-cucumerinum. Journal of Plant Pathology 99: 437-444.
• Starovic, M., Ristic, D., Pavlovic, S., Ristic, M., Stevanovic, M., AlJuhaimi, F., Svetlana, N., Ozcan, M.M. (2016). Antifungal activities of different essential oils against anise seeds mycopopulations. Journal of Food Safety and Food Quality-Archiv Fur Lebensmittelhygiene 67: 72-78.
• Wodnicka, A., Huzar, E., Dzieciol, M., Krawczyk, M. (2019). Comparison of the composition and fungicidal activity of essential oils from fennel fruits cultivated in Poland and Egypt. Polish Journal of Chemical Technology 21: 38-42.
• Woolf, A. (1999). Essential oil poisoning. Journal of Toxicology: Clinical Toxicology 37: 721-727.
• Yalçın, H., Akın, M., Sanda, M.A., Çakır, A. (2007). Gas chromatography/mass spectrometry analysis of Laurus nobilis essential oil composition of Northern Cyprus. Journal of Medicinal Food 10: 715-719.
• Yamini, Y., Sefidkon, F., Pourmortazavi, S.M. (2002). Comparison of essential oil composition of Iranian fennel (Foeniculum vulgare) obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Flavour and Fragrance Journal 17(5): 345-348.
• Yılmaz, B., Deniz, İ. (2017). The effects of cultivation area and altitude variation on the composition of essential oil of Laurus nobilis L. grown in eastern, Western and Central Karadeniz Region. International Journal Secondary Metabolite 4: 187-194.