In vitro anticancer, antimicrobial and antiradical properties and bioactive compounds of endemic Wiedemannia orientalis Fisch. & Mey. flowers

Yıl 2022, , 17 - 21, 12.12.2022

Serhat Keser , Fatma Keser , Suat Tekin , İsmail Türkoğlu , Omer Kaygılı , Ersin Demir , Prof. Dr. Ökkeş Yılmaz , Süleyman Sandal , Sevda Kırbağ , Mustafa Karatepe

https://doi.org/10.54565/jphcfum.1192938

Öz

W. orientalis is a one-year herbaceous plant, located in the Lamiaceae family, and is called Ballıbaba in Turkish. In the presented study, the antimicrobial and anticancer properties of flowers extracts of endemic W. orientalis were investigated for the first time. Also, it was investigated the antiradical activity and phytochemical contents of this plant extracts. According to our study results, endemic W. orientalis flowers extract show very high anticancer activity against MCF-7, HCT-116 and LNCaP cancer cell lines, high antiradical activity against ABTS radicals, and effective antimicrobial activity against some microorganism caused infection in humans. In conclusion, endemic W. orientalis can be used as an anticancer and antimicrobial agent and this plant can be the subject of further studies in the field of herbal medicine.

Anahtar Kelimeler

Anticancer, antimicrobial, antiradical, endemic, phytochemical, Wiedemannia

Destekleyen Kurum

TÜBİTAK

Proje Numarası

114Z124

Teşekkür

The present study was supported by TUBITAK, under grant number 114Z124.

Kaynakça

• [1] Pandey G, Madhuri S. 2009. Some medicinal plants as natural anticancer agents, Pharmacognosy Reviews. 3:259–263.
• [2] Modi C, Mody S, Patel H, Dudhatra G, Kumar A, Awale M. 2012. Herbal antibacterials: a review. Journal of Intercultural Ethnopharmacology. 1:52–61.
• [3] Keser S, Keser F, Kaygili O, Tekin S, Demir E, Turkoglu I, Turkoglu S, Parlak AE, Yilmaz O, Karatepe M, Sandal S, Kirbag S. 2020. Phytochemical compounds and antiradical, antimicrobial, and cytotoxic activities of the extracts from Hypericum scabrum L. flowerss. Natural Product Research. 34:714–719.
• [4] Larson RA. 1988. The antioxidants of higher plants. Pytochemistry. 27:969–978.
• [5] Pratt DE, Hudson BJF. 1990. Natural antioxidants not exploited commercially in food antioxidants. Hudson B.J.F. Ed.; (Elsevier; Amsterdam), pp. 171–192.
• [6] Ramarathnam N, Osawa T, Namiki M, Kawakishi S. 1988. Chemical studies on novel rice hull antioxidants. 1. Isolation, fractionation, and partial characterization. Journal of Agricultural and Food Chemistry. 36:732–737.
• [7] Davis PH. 1988. Flora of Turkey and the East Aegean Islands (Vol. 10). Edinburgh University Press, Edinburgh.
• [8] Unsalar Kolik T. 2006. Wiedemannia orientalis Fisch. & Mey. üzerinde farmakognozik araştırmalar (In Turkish). Master Thesis, Ataturk University, Health Sciences Institute. Erzurum, Turkey.
• [9] Albayrak S, Aksoy A. 2013. Biological activities of Wiedemannia multifada (Linnaeus) Bentham and Wiedemannnia orientalis Fisch. & Mey. Asian Pacific Journal of Tropical Biomedicine. 3:196–201.
• [10] Kilic O, Bagci E. 2014. Essential oil composition of Wiedemannia Fisch. & C.A. Mey. Genus from Turkey: A chemotaxonomic approach. Journal of Essential Oil Bearing Plants. 17:741–746.
• [11] Guvenalp Z, Ozbek H, Unsalar T, Kazaz C, Demirezer LO. 2006. Iridoid, flavonoid and phenylethanoid gylcosides from Wiedemannia orientalis. Turkish Journal of Chemistry. 30:391-400.
• [12] Baytop T. 1984. Geçmişte ve Bugün Türkiye’de Bitkiler ile Tedavi (In Turkish). İstanbul Üniversitesi Yayınları, Istanbul, Turkey.
• [13] Keser S. 2014. Antiradical activities and phytochemical compounds of firethorn (Pyracantha coccinea) fruit extracts. Natural Product Research. 28: 1789–1794.
• [14] Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology. 28: 25–30.
• [15] Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26: 1231–1237.
• [16] Halliwell B, Gutteridge JMC, Aruoma O. 1987. The deoxyribose method: a simple test tube assay for determination of rate constants for reactions of hydroxyl radicals. Analytical Biochemistry. 165: 215–219.
• [17] Slinkard K, Singleton VL. 1977. Total phenol analysis-automation and comparison with manual methods. American Journal of Enology and Viticulture. 28: 49–55.
• [18] Kim DO, Chun OK, Kim YJ, Moon HY, Lee CY. 2003. Quantification of polyphenolics and their antioxidant capacity in fresh plums. Journal of Agricultural and Food Chemistry. 51: 6509–6515.
• [19] Amaeze OU, Ayoola GA, Sofidiya MO, Adepoju-Bello AA, Adegoke AO, Coker HAB. 2011. Evaluation of antioxidant activity of Tetracarpidium conophorum (Mull. Arg) Hutch & Dalziel leaves. Oxidative Medicine and Cellular Longevity. Article ID 976701, 7 pages.
• [20] Zu YG, Li CY, Fu YJ, Zhao CJ. 2006. Simultaneous determination of catechin, rutin, quercetin, kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaf by RP-HPLC with DAD. Journal of Pharmaceutical and Biomedical Analysis. 41: 714–719.
• [21] Christie WW. 1992. Gas chromatography and lipids. The Oil Press, Glasgow.
• [22] Sanchez-Machado DI, Lopez-Hernandez J, Paseiro-Losado P. 2002. High performance liquid chromatographic determination of alpha-tocopherol in macroalgae. Journal of Chromatography A. 976: 277–284.
• [23] López-Cervantes J, Sánchez-Machado DI, Ríos-Vázquez NJ. 2006 High performance liquid chromatography method for the simultaneous quantification of retinol, α-tocopherol, and cholesterol in shrimp waste hydrolysate. Journal of Chromatography A. 1105: 135–139.
• [24] Collins CM, Lyne PM. 1989. Microbiological Methods, Buttermorths-Heinemann, London, England.
• [25] Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 65: 55–63.
• [26] Denizot F, Lang R. 1986. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of Immunological Methods. 89: 271–277.
• [27] Turkoglu I. 2018. Screening of total phenolic, antiradical and antioxidant activities of Wiedemannia orientalis. Cellular and Molecular Biology. 64:42–46.