Lentinus edodes, Lactarius delicious ve Ganoderma lucidum’un antibiyofilm ve antimikrobiyal etkinlikleri

Yıl 2017, Cilt: 17 Sayı: 4, 660 - 668, 20.12.2017

Başar Karaca , Ilgaz Akata , Arzu Çöleri Cihan

https://doi.org/10.17475/kastorman.341971

Cited By: 5

Öz

Çalışmanın amacı: Bu çalışma kapsamında tıbbi önemi olan üç makrofungus
türünün [(Lentinus edodes (Berk.)
Sing. (Shiitake), Lactarius deliciosus
Fr. ve Ganoderma lucidum (Curtis) P. Karst.)] metanolik
(%60) ve etanolik (%95) özütlerinin Pseudomonas
aeruginosa ve Salmonella
Typhimurium’daki antibakteriyal ve biyofilm oluşumunu engelleme (antibiyofilm) potansiyelleri
araştırılmıştır.

Materyal ve Yöntem: Antimikrobiyal aktivitelerin
değerlendirilmesinde standart agar kuyu difüzyon, minimum inhibisyon
konsantrasyon (MİK) ve minimum bakterisidal konsantrasyon (MBK) testleri
kullanılmıştır. Makrofungus özütlerinin biyofilm oluşumu üzerine (antibiyofilm)
olan etkilerinin değerlendirmesinde kristal viyole bağlanma yöntemi esas
alınmıştır.

Temel sonuçlar: Makrofungus örneklerinin
yalnızca metanolik özütlerinde antimikrobiyal etkinlik saptanmıştır.
Antibiyofilm aktivite değerlendirmesindeyse en yüksek aktivite G. lucidum’da
görülmüştür.

Araştırma vurguları: Elde edilen bulgular,
doğal antimikrobiyal ajanların araştırılmasında sıklıkla tercih edilen tıbbi makrofungus
türlerinin, biyofilmlerle mücadele araştırmalarında da yüksek potansiyel
içermelerinden ötürü tercih edilebileceklerini kanıtlar mahiyettedir.

Anahtar Kelimeler

Makrofungus, Biyofilm, Antimikrobiyal, Antibiyofilm

Antimicrobial and Antibiofilm Activities of Lentinus edodes, Lactarious delicious, and Ganoderma lucidum

Öz

Aim of study: In this
study, the antimicrobial and antibiofilm potentials of methanolic (60%) and
ethanolic (95%) extracts of three medicinal macrofungi species  [(Lentinus
edodes (Berk.) Sing. (Shiitake), Lactarius
deliciosus Fr., and Ganoderma lucidum
(Curtis) P. Karst.)]
on Pseudomonas aeruginosa, and Salmonella
Typhimurium were investigated.

Material and Methods: Standart agar well
diffusion, minimum inhibitory concentration, and minimum bactericidal
concentration tests were performed to evaluate the antimicrobial activities.
The effects of macrofungi extracts on biofilm formation (antibiofilm) were
evaluated based on crystal violet binding assay.

Main
Results: Antimicrobial and
antibiofilm activities were determined only in methanolic extracts of macrofungi samples. The highest antibiofilm activity was observed in G. lucidum methanolic
extract.

Research highlights: Obtained findings
proved that the medicinal macrofungi species frequently preferred in the
investigation of natural antimicrobial agents, and could be preferred for
surveys of biofilm control due to their high potentials.  

Anahtar Kelimeler

Macrofungi, Biofilm, Antimicrobial

Kaynakça

• Alves, M. J., Ferreira, I. C., Lourenço, I., Costa, E., Martins, A., & Pintado, M. (2014). Wild mushroom extracts as inhibitors of bacterial biofilm formation. Pathogens, 3(3), 667-679.
• Bin, L., Wei, L., Xiaohong, C., Mei, J., & Mingsheng, D. (2012). In vitro antibiofilm activity of the melanin from Auricularia auricula, an edible jelly mushroom. Annals of microbiology, 62(4), 1523-1530.
• Boucher, H. W., Talbot, G. H., Bradley, J. S., Edwards, J. E., Gilbert, D., Rice, L. B., ... & Bartlett, J. (2009). Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clinical Infectious Diseases, 48(1), 1-12.
• Buommino, E., Scognamiglio, M., Donnarumma, G., Fiorentino, A., & D’Abrosca, B. (2014). Recent advances in natural product-based anti-biofilm approaches to control infections. Mini reviews in medicinal chemistry, 14(14), 1169-1182.
• Carson, L., Chau, P. K., Earle, M. J., Gilea, M. A., Gilmore, B. F., Gorman, S. P., ... & Seddon, K. R. (2009). Antibiofilm activities of 1-alkyl-3-methylimidazolium chloride ionic liquids. Green Chemistry, 11(4), 492-497.
• Clardy, J., & Walsh, C. (2004). Lessons from natural molecules. Nature, 432(7019), 829.
• Clatworthy, A. E., Pierson, E., & Hung, D. T. (2007). Targeting virulence: a new paradigm for antimicrobial therapy. Nature chemical biology, 3(9), 541-548. CLSI. (2003). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard Seventh Edition, CLSI Document M7-A7, Pennsylvania: USA.
• Cos, P., Vlietinck, A. J., Berghe, D. V., & Maes, L. (2006). Anti-infective potential of natural products: how to develop a stronger in vitro ‘proof-of-concept’. Journal of ethnopharmacology, 106(3), 290-302.
• de Carvalho, M. P., Gulotta, G., do Amaral, M. W., Lünsdorf, H., Sasse, F., & Abraham, W. R. (2016). Coprinuslactone protects the edible mushroom Coprinus comatus against biofilm infections by blocking both quorum‐sensing and MurA. Environmental microbiology, 18(11), 4254-4264.
• de la Fuente-Núñez, C., Reffuveille, F., Fernández, L., & Hancock, R. E. (2013). Bacterial biofilm development as a multicellular adaptation: antibiotic resistance and new therapeutic strategies. Current opinion in microbiology, 16(5), 580-589.
• Hawkey, P. M. (2008). The growing burden of antimicrobial resistance. Journal of antimicrobial chemotherapy, 62(suppl_1), i1-i9.
• Karaman, M., Firinci, F., Ayyildiz, Z. A., & Bahar, İ. H. (2013). Pseudomonas aeruginosa Suşlarında İmipenem, Tobramisin ve Curcuminin Biyofilm Oluşumu Üzerine Etkisi. Mikrobiyol Bul, 47(1), 192-194.
• O'Toole, G. A., Pratt, L. A., Watnick, P. I., Newman, D. K., Weaver, V. B., & Kolter, R. (1999). [6] Genetic approaches to study of biofilms. Methods in enzymology, 310, 91-109.
• Petrović, J., Glamočlija, J., Stojković, D., Nikolić, M., Ćirić, A., Fernandes, A., ... & Soković, M. (2014). Bioactive composition, antimicrobial activities and the influence of Agrocybe aegerita (Brig.) Sing on certain quorum-sensing-regulated functions and biofilm formation by Pseudomonas aeruginosa. Food & function, 5(12), 3296-3303.
• Pitts, B., Hamilton, M. A., Zelver, N., & Stewart, P. S. (2003). A microtiter-plate screening method for biofilm disinfection and removal. Journal of microbiological methods, 54(2), 269-276.
• Prouty, A. M., & Gunn, J. S. (2003). Comparative analysis of Salmonella enterica serovar Typhimurium biofilm formation on gallstones and on glass. Infection and immunity, 71(12), 7154-7158.
• Signoretto, C., Marchi, A., Bertoncelli, A., Burlacchini, G., Papetti, A., Pruzzo, C., ... & Spratt, D. A. (2014). The anti-adhesive mode of action of a purified mushroom (Lentinus edodes) extract with anticaries and antigingivitis properties in two oral bacterial pathogens. BMC complementary and alternative medicine, 14(1), 75.
• Solak, M. H., Kalmis, E., Saglam, H., & Kalyoncu, F. (2006). Antimicrobial activity of two wild mushrooms Clitocybe alexandri (Gill.) Konr. and Rhizopogon roseolus (Corda) TM Fries collected from Turkey. Phytotherapy Research, 20(12), 1085-1087.
• Solmaz, G., Ozen, F., Ekinci, Y., Bird, P. S., & Korachi, M. (2013). Inhibitory and disruptive effects of shiitake mushroom (Lentinula edodes) essential oil extract on oral biofilms. Jundishapur Journal of Microbiology, 6(9).
• Spellberg, B., Guidos, R., Gilbert, D., Bradley, J., Boucher, H. W., Scheld, W. M., ... & Infectious Diseases Society of America. (2008). The epidemic of antibiotic-resistant infections: a call to action for the medical community from the Infectious Diseases Society of America. Clinical Infectious Diseases, 46(2), 155-164.
• Stepanović, S., Vuković, D., Dakić, I., Savić, B., & Švabić-Vlahović, M. (2000). A modified microtiter-plate test for quantification of staphylococcal biofilm formation. Journal of microbiological methods, 40(2), 175-179.
• Turkoglu, A., Duru, M. E., Mercan, N., Kivrak, I., & Gezer, K. (2007). Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chemistry, 101(1), 267-273.
• Url:http://www.who.int/mediacentre/factsheet/fs139/en/.2014 :(giriş tarihi: 12 Kasım 2016).
• Üstün, O. (2011). Makrofungusların besin değeri ve biyolojik etkileri. Turk Hij Den Biyol Derg, 68(4), 223-40.
• Veldman, K., Cavaco, L. M., Mevius, D., Battisti, A., Franco, A., Botteldoorn, N., ... & Guerra, B. (2011). International collaborative study on the occurrence of plasmid-mediated quinolone resistance in Salmonella enterica and Escherichia coli isolated from animals, humans, food and the environment in 13 European countries. Journal of Antimicrobial Chemotherapy, 66(6), 1278-1286.
• Vestby, L. K., Møretrø, T., Langsrud, S., Heir, E., & Nesse, L. L. (2009). Biofilm forming abilities of Salmonella are correlated with persistence in fish meal-and feed factories. BMC veterinary research, 5(1), 20.
• Wilkins, M., Hall-Stoodley, L., Allan, R. N., & Faust, S. N. (2014). New approaches to the treatment of biofilm-related infections. Journal of Infection, 69, S47-S52.