Morphological and Molecular Characterization of Hebeloma subtortum (Hymenogastraceae), a New Record Macrofungus from Bingöl Province, Turkey

Yıl 2019, Cilt: 19 Sayı: 1, 1 - 10, 27.03.2019

Ayten Dizkırıcı , İsmail Acar Ayşenur Kalmer Yusuf Uzun

https://doi.org/10.17475/kastorman.543375

Cited By: 2

Öz

Aim of Study: The specimen identified as Hebeloma subtortum based on both morphological and molecular characterizations. Area of Study: Samples were collected from Bingöl province and the study was conducted at the Department of Molecular Biology and Genetics in Van Yüzüncü Yıl University.

Material and Methods: Characters of pileus, stipe, lamellae and basidia, cystidia, spores were used as macroscopic and microscopic features, respectively. DNA sequences of two loci including the internal transcribed spacer (ITS) region and the large subunit (LSU) of nuclear ribosomal RNA gene were used to show the evolutionary relationship and taxonomic position of the species within Hebeloma genus. The DNA sequences of the above-mentioned regions of H. subtortum were compared to those of the same and different species of the genus downloaded from NCBI. Main results: In phylogenetic analyses, H. subtortum distinctly clustered with its representatives retrieved from NCBI with high bootstrap value. The ITS tree was more informative compared to LSU. Hebeloma subtortum closely grouped with H. mesophaeum in the ITS tree. Research highlights: Hebeloma subtortum has been described and illustrated as a new record from Turkey.

Anahtar Kelimeler

Hebeloma, ITS, mycogenetics, phylogeny

Bingöl ilinden yeni bir kayıt olarak Hebeloma subtortum (Hymenogastraceae) türünün morfolojik ve moleküler karakterizasyonu

Öz

Çalışmanın Amacı: Hebeloma subtortum türü hem morfolojik hem de moleküler karakterizasyonlara dayanarak tanımlanmıştır.

Çalışma Alanı: Örnekler Bingöl ilinden toplanmış ve çalışma Van Yüzüncü Yıl Üniversitesi Moleküler Biyoloji ve Genetik Bölümü'nde yapılmıştır.

Gereç ve Yöntemler: Şapka, sap, lamel yapıları ve bazidya, sistidya, spor yapıları sırasıyla makroskopik ve mikroskopik özellikler olarak kullanılmıştır. Nükleer ribozomal iç aralayıcı bölge (nrITS) ve ribozomal en büyük alt birim (LSU) olmak üzere iki DNA bölgesi çalışılan türün evrimsel ilişkisini ve Hebeloma cinsi içindeki taksonomik konumunu göstermek için kullanılmıştır. Çalışılan türün DNA dizileri, NCBI very tabanından indirilen cinse ait aynı ve farklı türlerle karşılaştırılmıştır.

Ana sonuçlar: Filogenetik analizlerde, H. subtortum, yüksek bootstrap değeri ile NCBI'den alınan temsilcileri ile belirgin bir şekilde kümelenmiştir. ITS ağacı, LSU'ya kıyasla daha bilgilendirici olmuştur. H. subtortum, ITS ağacında H. mesophaeum ile yakın gruplanmıştır.

Araştırma konuları: Hebeloma subtortum, Türkiye'den yeni bir kayıt olarak tanımlanmış ve gösterilmiştir.

Anahtar Kelimeler

Hebeloma, ITS, mikogenetik, filogeni

Kaynakça

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