Total Phenolic and Total Flavonoid Compounds and Ion Chromatographic Anions and Cations Determinations of Giresun Region’s Sakarca Plant (Ornithogalum Umbellatum L.)

Yıl 2017, , 152 - 160, 15.12.2017

Elif Apaydın , Murat Yolcu

https://doi.org/10.31466/kfbd.344363

Cited By: 2

Öz

In this work; total phenolic compounds and total
flovonoid compounds have been identificated of the fresh and diet of Sakarca (Ornithogalum umbellatum L.) plant which has
been abundantly grown in Giresun. Fresh and cooked plant samples were shredded
and extracted with soxhalet in methanol. Total phenolic contents of the were
determined by Folin Ciocalteu method and total flavonoid contents of the
studied samples  were determined by
aluminum nitrate method. In addition, some anion-cation content of samples were
simultaneously determined by the studied samples Dionex ICS-5000 ion
chromatography system.

Anahtar Kelimeler

Ornithogalum umbellatum L., sakarca, phenolic compound, flavonoid compound, ion chromatography

Giresun Yöresine Ait Sakarca (Ornithogalum Umbellatum L.) Bitkisinde Toplam Fenolik Madde, Toplam Flavonoid Madde ve İyon Kromatografik Anyon-Katyon İçeriklerinin Tayinleri

Öz

Bu çalışmada Giresun yöresinde bolca yetişen sakarca
(Ornithogalum umbellatum L.)
bitkisinin taze ve diyet olarak kullanılan halinin toplam fenolik madde, toplam
flavonoid madde ve anyon-katyon içeriklerinin tayini amaçlanmıştır. Taze ve
ısıl işlem görmüş bitki numuneleri doğrayıcı ile küçültülmüş ve metanol ile
soxhlet ekstraksiyonu yapılmıştır. Bitki ekstraktlarında toplam fenolik madde
tayini Folin Ciocalteu yöntemiyle ve toplam flavonoid madde tayini ise alüminyum
nitrat yöntemi ile yapılmıştır. Ayrıca sakarca bitkisinin bazı anyon-katyon
içeriği Dionex ICS-5000 iyon kromatografi sistemi ile eşzamanlı olarak belirlenmiştir

Anahtar Kelimeler

sakarca, fenolik madde, flavonoid madde, Ornithogalum umbellatum L., iyon kromatografi

Kaynakça

• Anonim (2013). http://otekiyuz.com/ornithogalum-umbellatum-namidiger-sakarca/ (Erişim tarihi: 10.08. 2017)
• Düzgüner, V. (2005) Deneysel olarak diyabet oluşturulan tavşanlarda çinkonun lipid peroksidasyonu ve antioksidan sistem üzerine etkisi, Yüksek Lisans Tezi, Mustafa Kemal Üniversitesi, Sağlık Bilimleri Enstitüsü, Hatay.
• Gamez-Meza, N., Noriega-Rodriguez, J.A., Medina-Juarez, L.A., Ortega-Garcia, J., Cazarez-Casanova, R., Angulo-Guerrero, O. (1999). The Journal of the American Oil Chemists' Society, 76, 1445.
• Ghosh, J., Myers, C.E. (1998). Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells, Proc Natl Acad Sci, 95: 13182-7.
• Haddad P.R., Jackson P.E. (1990). Ion chromatography: Principles and Applications, Elsevier Science Publishers B.V., 775.
• Huang, D., Ou, B. and Prior, R. L. (2005). The Chemistry Behind Antioxidant Capacity Assays, Journal of Agriculture and Food Chemistry,53, 1841-1856.
• Moreno M.I.N., Isla M.I., Sampietro A.R., Vattuone M.A(2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacol; 71: 109–114.
• Özen, T. (2010). Antioxidant activity of wild edible plants in the Black Sea Region of Turkey. Grasas y Aceites, Vol 61, No 1, 86-94.
• Rice-Evans, C.A., Miller, N.J., Paganga, G.(1997). Antioxidant properties of phenolic compounds, Trends in Plant Science, 2, 152-159.
• Shahidi F. (1997) Natural Antioxidant: Chemistry, Health Effects and Applications, AOCS Press, Champaigh, 25:124-4.
• Yin, G.Y., Yin, Y.F., He, X.F. (1995). Effect of zhuchun pill on immunity and endocrine function of elderly with kidney-yang deficiency, Chung Kuo Chung Hsi I Chieh Ho Tsa Chih, 15: 601-3.