Xyleborinus saxesenii (Coleoptera: Curculionidae)’den İzole Edilen Bakterilerin Tanımlanması

Yıl 2017, Cilt: 7 Sayı: 1, 85 - 101, 15.06.2017

Hatice Katı , Ahmet Katı Serpil Uğraş , Hüseyin Yılmaz

Öz

Yazıcı böceklerden, Xyleborinus saxesenii Ratzeburg (Coleoptera: Curculionidae) Doğu
Karadeniz bölgesinde fındık bahçelerinde ciddi zararlara neden olmaktadır.
Zararlı böceklere karşı biyolojik mücadele ajanı geliştirmek için böceklerden
izole edilen bakteriler tanımlanmaktadır. Bu çalışmada, X. saxesenii’den izole edilen bakteriler morfolojik, fizyolojik,
biyokimyasal, yağ asit analizi ve moleküler olarak incelenmiştir. Sonuç olarak,
bakteriler Rahnella aquatilis (Xs1), Pseudomonas putida (Xs2), Sphingobacterium multivorum (Xs3), Serratia liquefaciens (Xs4), Aeromonas sp. (Xs5), Hafnia alvei (Xs6, Xs7), Acinetobacter calcoaceticus (Xs8), Pseudomonas sp. (Xs9), Pseudomonas fluorescens (Xs10, Xs13), Pantoea agglomerans (Xs11) ve Serratia marcescens (Xs12) olarak
tanımlanmıştır.

Anahtar Kelimeler

Gram (-) bakteri, fındık, zararlı, Xyleborinus saxesenii

Kaynakça

• Ak, K., Uysal, M., and Tuncer, C. (2005a). Bark Beetle (Coleoptera: Scolytidae) species which are harmful in hazelnut orchards, their short biology and densities in Giresun, Ordu and Samsun provinces of Turkey. Journal of Agricultural Faculty of Ondokuz Mayıs University, 20, 37-44.
• Ak, K., Uysal, M., and Tuncer, C. (2005b). The injury level of Bark Beetles (Coleoptera: Scolytidae) in hazelnut orchards in Giresun, Ordu and Samsun provinces of Turkey. Journal of Agricultural Faculty of Gaziosmanpaşa University, 22, 9-14.
• Ak, K., Uysal, M., Tuncer, C., and Akyol., H. (2005c). Bark beetle species (Col.: Scolytidae) harmful on hazelnut In Middle and East Black Sea Region of Turkey and their control strategies. Journal of Agricultural Faculty, Selcuk University, 19, 37-39.
• Altschul, S.F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215, 403-410.
• Bahar, A. A., and Demirbağ, Z. (2007). Isolation of pathogenic bacteria from Oberea linearis (Coleptera: Cerambycidae). Biologia, 62, 13-18.
• Benson, H. J. (1985) Microbiological Applications a aboratory manual in general Microbiology. W.C. Brown publishers.
• Berge, O., Heulin, T., Achouak, W., Richard,C., Bally, R., and Balandreau, J. (1991). Rahnella aquatilis, a nitrogen-fixing enteric bacterium associated with the rhizosphere of wheat and maize. Canadian Journal of Microbiology, 37, 195-203.
• Bouvet, P. J. M., and Grimont, P. A. D. (1986). Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter hemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. International Journal of Systematic Bacteriology, 36, 228-240.
• Brenner, D.J., Müller, H.E., Steigerwalt, A.G., Whitney, A.M., O’Hara, C.M., and Kämpfer, P. (1998). Two new Rahnella genomospecies that cannot be phenotypically differentiated from Rahnella aquatilis. International Journal of Systematic Bacteriology, 48, 141-149.
• Brosius, J., Palmer, M. L., Kennedy, P. J., and Noller, H. F. (1978). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America, 75, 4801-4805.
• Bucher, G. E. (1981). Microbial Control of Pests and Plant Diseases, 1970-1980. Burges H. D. (Ed.), pp. 7-33, Academic Press, New York.
• Cankar, K., Kraigher, H., Ravnikar, M., and Rupnik, M. (2005). Bacterial endophytes from seeds of Norway spruce (Picea abies L. Karst). FEMS Microbiology Letters, 244, 341-345.
• Cappuccino, J. G., and Sherran, N. (1992). Microbiology, a Laboratory Manual (3rd ed.). Rockland Community College, Suffern, New York.
• Claus, M.A. (1992). Standardized Gram staining procedure. World Journal of Microbiology and Biotechnology, 8, 451–452.
• Erturk, O., and Demirbağ, Z. (2006). Studies on bacterial flora and biological control Agent of Cydia pomonella L. (Lepidoptera: Tortricidae). African Journal of Biotechnology, 5, 2081-2085.
• Farmer, 3rd., J.J., Davis, B. R., Hickman-Brenner, F.W., and et al. (1985). Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical specimens. Journal of Clinical Microbiology, 21, 46-76.
• Gavini, F., Mergaert, J., Christine, B.A., Mielcarek, C., Izard, D., Kersters, K., and Ley. J. (1989). Transfer of Enterobacter agglomerans (Beijerinck 1888) Ewing and Fife 1972 to Pantoea gen. nov. as Pantoea agglomerans comb. nov. and description of Pantoea dispersa sp. nov. International Journal of Systematic Bacteriology, 39, 337-345.
• Gokce, C., Sevim, A., Demirbag Z., and Demir, I. (2010). Isolation, characterization and pathogenicity of bacteria from Rhynchites bacchus (Coleoptera: Rhynchitidae). Biocontrol Science and Technology, 20, 973-982.
• Gray, S.J., Stickler, D. J., and Bryant, T.N. (1990). The incidence of virulence factors in mesophilic Aeromonas species isolated from farm animals and their environment. Epidemioogy and Infection, 105, 277–294.
• Gupta, A.K., Nayduch, D., Verma, P., Shah, B., Ghate, H.V., Patole, M.S., and Shouche, Y.S. (2012). Phylogenetic characterization of bacteria in the gut of house flies (Musca domestica L.). FEMS Microbiology Ecology, 79, 581–593.
• Hashidoko, Y., Itoh, E., Yokota, K., Yoshida, T., and Tahara, S. (2002). Characterization of five phyllosphere bacteria isolated from Rosa rugosa leaves, and their phenotypic and metabolic properties. Bioscience, Biotechnology, and Biochemistry, 66, 2474-2478.
• Heulin, T., Berge, O., Mavingui, P. Gouzou, L., Hebbar K.P., and Balandreau, J. (1994). Bacillus polymyxa and Rahnella aquatilis, the dominant N2-fixing bacteria associated with wheat rhizosphere in French soils. European Journal of Soil Biology, 30, 35-42.
• Ince, I.A., Kati, H., Yılmaz, H., Demir, I., and Demirbaǧ, Z. (2008). Isolation and identification of bacteria from Thaumetopoea pityocampa Den. and Schiff. (Lep., Thaumetopoeidae) and determination of their biocontrol potential. World Journal of Microbiology and Biotechnology, 24, 3005-3015.
• Isık, U. M., Ecevit, O., Kurt, M.A., and Yücetin, T. (1987). Researchs on application of integrated pest management method at hazelnut plantations in the eastern black-sea region in Turkey. Ondokuzmayıs University publication, 20, 81-83.
• Ivanov, G. M.,and Gukasjan, A. B. (1966). Charakteristika Nekatorych Stammov Roda Pseudomonas vydelennych iz Nasekomych, 135, In: A. B. Gukasjan (Ed.) Mikroorganizmy v Borbe s Vrediteljami Lesnogo Chozjajstva, Nauka, Moskva.
• Jackson, T. A., Boucias, D. G., and Thaler, J. O. (2001). Pathobiology of amber disease, caused by Serratia spp., in the New Zeland Grass Grub, Costelytra zealandica. Journal of Invertebrate Pathology, 78, 232-243.
• Jeyaprakash, A., Hoy, M. A., and Allsopp, M. H. (2003). Bacterial Diversity in worker adults of Apis mellifera capensis and Apis mellifera scutellata (Insecta: Hymenoptera) assessed using 16S rRNA sequences. Journal of Invertebrate Pathology, 84, 96-103.
• Kati, H., Ince, I.A., Sezen, K., Isci S., and Demirbag, Z. (2009). Characterization of two Bacillus thuringiensis ssp. morrisoni strains isolated from Thaumetopoea pityocampa (Lep., Thaumetopoeidae). Biocontrol Science and Technology, 19, 475-484.
• Klein, M. G., and Kaya, H. K. (1995). Bacillus and Serretia species for scarab control. Memórias do Instituto Oswaldo Cruz, 90, 87-95.
• Kılıç, O. (1994). Fındıkta Dönüm Noktası. Tarım ve Köy Bakanlığı Dergisi, 97, 38-40.
• Kurt, M.A. (1982). Doğu Karadeniz Bölgesinde fındık zararlıları, tanınmaları, yayılış ve zararları, yaşayışları ve savaşım yöntemleri. T.C. Tarım ve Orman Bakanlığı, Zir. Müc. Zir. Kar. Gen. Müd., Samsun Bölge Zir. Müc. Araş. Enst., Mesleki Kitaplar Serisi, No: 26, Ankara.
• Kuzina, L. V., Peloquin, J. J., Vacek, D. C., and Miller, T. A. (2001). Isolation and identification of bacteria associated with adult laboratory mexican fruit flies, Anastrepha ludens (Diptera: Tephritidae). Current Microbiology, 42, 290-294.
• Lacey, L. A., Unruh, T. R., Simkins, H., and Thomsen-Archer, K. (2007). Gut bacteria associated with the pacific coast wireworm, Limonius canus, inferred from 16S rDNA sequences and their implications for control. Phytoparasitica, 35, 479-489.
• Lindow, S. E., Desurmont, C., Elkins, R., McGourty, G., Clark, E., and Brandl, M. T. (1998). Occurrence of indole-3-acetic acid producing bacteria on pear trees and their association with fruit russet. Phytopathology, 88, 1149-1157.
• Lipa, J.J., and Wiland, E. (1972). Bacteria isolated from cutworms and their infectivity to Agrotis sp. Acta Microbiologica Polonica, 4, 127–140.
• Lyapunov, Ya. E., Kuzyaev, R.Z., Khismatullin, R. G., and Bezgodova, O. A. (2008). Intestinal Enterobacteria of the hibernating Apis mellifera mellifera L. Bees. Microbiology, 77, 373-379.
• Martinez, A. J., Robacker, D. C., Garcia, J. A., and Esau, K. L. (1994). Laboratory and field olfactory attraction of the mexican fruit fly (Diptera: Tephritidae) to metabolities of bacterial species. Florida Entomologist, 77, 117-126.
• Muratoglu, H., Sezen, K., and Demirbağ, Z. (2011a). Determination and pathogenicity of the bacterial flora associated with the spruce bark beetle, Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae). Turkısh Journal of Biology, 35, 9-20.
• Muratoglu, H., Demirbag, Z., and Sezen, K. (2011b). The first investigation of the diversity of bacteria associated with Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Biologia, 66, 288-293.
• Murrell A., Dobson, S. J., Yang, X., Lacey, E., and Barker, S. C. (2003). A survey of bacterial diversity in ticks, lice and fleas from Australia. Parasitology Research, 89, 326–334.
• Nayduch D., Honko, A., Noblet, G. P., and Stutzenberger, F. (2001). Detection of Aeromonas caviae in the common housefly Musca domestica by culture and polymerase chain reaction. Epidemiology and Infection, 127, 561-566.
• Nelson, K. E., Weinel, C., Paulsen, I. T., and et al. (2002). Complete Genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environmental Microbiology, 4, 799-808.
• Niemi, R. M., Heikkilä, M. P., Lahti, K., Kalso, S., and Niemelä, S. I. (2001). Comparison of methods for determining the numbers and species distribution of coliform bacteria in well water samples. Journal of Applied Microbiology, 90, 850-858.
• O’Callaghan, M., and Jackson, T. A. (1993). Isolation and enumeration of Serratia entomophila, a bacterial pathogen of the New Zealand Grass Grub, Costelytra zealandica. Journal of Applied Bacteriology, 75, 307-314.
• Osborn, F., Berlioz, L., Vitelli-Flores, J., Monsalve, W., Dorta, B., and Lemoine, V. R. (2002). Pathogenic effects of bacteria isolated from larvae of Hylesia metabus Crammer (Lepidoptera: Saturniidae). Journal of Invertebrate Pathology, 80, 7-12.
• Peter, G. 1984. Plant Pests and Their Control, Fenemore, London.
• Pidiyar, V. J., Jangıd, K., Patole, M. S., and Shouche. Y. S. (2004). Studies on cultured and uncultured microbiota of wild Culex quinquefasciatus mosquito midgut based on 16S ribosomal RNA gene analysis. American Journal of Tropical Medicine and Hygiene, 70, 597-603.
• Prado, M. A., Pimenta, F. C., Hayashid, M., Souza, P. R., Pereira, M. S., and Gir, E. (2002). Enterobacteria isolated from cockroaches (Periplaneta americana) captured in a Brazilian hospital. Revista Panamericana de Salud Pública, 11, 93-98.
• Rahuma, N., Ghenghesh, K.S., Ben Aissa, R., and Elamaari, A. (2005). Carriage by the housefly (Musca domestica) of multiple antibiotic- resistant bacteria that are potentially pathogenic to humans, in hospital and other urban environments in Misurata, Libya. Annals of Tropical Medicine and Parasitology, 99, 795-802.
• Sambrook J., Fritsch, E. F., and Maniatis, T. (1989). Molecular cloning: a laboratory manual (2nd ed.) New York, Cold Spring Harbor Laboratory Press.
• Sasser, M. S. (1990). Identification of by gas chromatography of cellular fatty acids, Technical Note 101. Newark, DE, Microbial ID, Inc.
• Selmi, E. (1998). Türkiye Kabuk Böcekleri ve Savaşı. İstanbul Üniv. Yayın No: 4042, Emek Matbaası, İstanbul. 196s.
• Sevim, A., Demirbağ, Z., and Demir, İ. (2010). A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities. Turkish Journal of Agriculture and Forestry, 34, 333-342.
• Sevim, E., Çelebi, O., and Sevim, A. (2012). Determination of the bacterial flora as a microbial control agent of Toxoptera aurantii (Homoptera: Aphididae). Biologia, 67, 397-404.
• Sezen, K., and Demirbağ, Z. (1999). Isolation and insecticidal activity of some bacteria from the hazelnut beetle (Balaninus nucum L.). Applied Entomology and Zoology, 34, 85-89.
• Sezen, K., and Demirbağ, Z. (2001). Insecticidal potential of Serratia marcescens Bn10. Biologia, 56, 333-336.
• Sezen K., Demir, I., and Demirbag, Z. (2004). Study of the bacterial flora as a biological control agent of Agelastica alni L. (Coleoptera: Chrysomelidae). Biologia, 59, 327-331.
• Sezen K., Demir, I., Kati, H., and Demirbag, Z. (2005). Investigations on bacteria as a potential biological control agent of summer chafer, Amphimallon solstitiale L. (Coleoptera: Scarabaeidae). Journal of Microbiology, 43, 463-468.
• Sezen K., Demir, I., and Demirbag, Z. (2007a). Identification and pathogenicity of entomopathogenic bacteria from common cockchafer, Melolontha melolontha (Coleoptera: Scarabaeidae). New Zealand Journal of Crop and Horticultural Science, 35, 79-85.
• Sezen, K., and Demirbag, Z. (2007b). The investigation of biological control agent of summer cockchafer (Melolontha melolontha, Coleoptera: Scarabaeidae). Ekoloji, 16, 34-40.
• Sezen, K., Kati, H., Nalcacioglu, R., Muratoglu, H., and Demirbag, Z. (2008). Identification and pathogenicity of bacteria from European shot-hole borer, Xyleborus dispar Fabricius (Coleoptera: Scolytidae). Annals of Microbiology, 58, 173-179.
• Sikorowski, P. P., and Lawrence, A. M. (1998). Transmission of Serratia marcescens (Enterobacteriaceae) in Adult Heliothis virescens (Lepidoptera: Noctuidae) Laboratory Colonies. Biological control, 12, 50-55.
• Sneath , A. P., Mair, N. S., Sharge, M. S. and Holt, J. G. (Eds.) (1986). Bergey’s Manual of Systematic Bacteriology 2, Williams and Wilkins, Baltimore.
• Steinhaus, E. A. (1963). Insect Pathology: An Advanced Treatise, Vol. 2, Academic Press, New York.
• Timmis, K. N. (2002). Pseudomonas putida: A cosmopolitan opportunist par excellence. Environmental Microbiology, 4, 779-781.
• Yamoah, E., Jones, E. E., Weld, R. J., Suckling, D. M., Waipara, N., Bourdôt, G.W., Hee, A.K.W., and Stewart, A. 2008. Microbial population and diversity on the exoskeletons of four insect species associated with gorse (Ulex europaeus L.). Australian Journal of Entomology, 47, 370-379.
• Yılmaz, H., Sezen, K., Kati, H., and Demirbağ, Z. (2006). The first study on the bacterial flora of the European spruce bark beetle, Dendroctonus micans (Coleoptera: Scolytidae). Biologia, 61, 679-686.
• Wood, S.L. and Bright, D.E. (1992). A catalog of Scolytidae and Platypodidae (Coleoptera), Part 2: Taxonomic index. Volumes A and B. Great Basin Naturalist Memoirs, 13, 1-1553.