Mathematical modeling of Escherichia coli inactivation in orange juice fermented with water kefir microorganisms

Yıl 2019, Cilt: 9 Sayı: 2, 297 - 310, 15.12.2019

Selin Kalkan

https://doi.org/10.31466/kfbd.640727

Cited By: 2

Öz

Water kefir is a homemade fermented beverage based on a sucrose solution with different dried and fresh fruits. The predominant flora in this beverage prepared with water kefir grains is lactic acid bacteria and some yeast species. The aim of study was mathematically express of E.coli inactivation by linear (simple linear, quadratic and cubic) and nonlinear models (Logistics, Gompertz and Brody) in fermented orange juice with water kefir grains and to determine the model suitability. For this purpose, during the storage period for 4 days under refrigerator conditions, E.coli level was determined daily in the control group (orange juice) and orange juice samples fermented with water kefir grains. As a result, it was determined that E.coli inactivation was 4.82 log cfu /mL in control group samples and 5.38 log cfu/mL in fermented orange juice samples. When the model comparison is made based on the calculated determination coefficients (R2) and error squares mean (HKO) of the used models, Cubic model as a linear model was found to be most suitable models with 0.998 R2 and 0.025 HKO; Brody model was found to be the most suitable model from nonlinear models with 0.995 R2 and 0.053 HKO values.

Anahtar Kelimeler

Water kefir, orange juice, Escherichia coli, mathematical modeling

Su kefiri mikroorganizmaları ile fermente edilen portakal suyunda Escherichia coli inaktivasyonunun matematiksel modellemesi

Öz

Su kefiri, farklı taze ve kuru meyvelerden elde edilen, sakkaroz bazlı ev yapımı fermente bir içecektir. Su kefiri taneleri ile hazırlanan bu içecekte baskın florayı laktik asit bakterileri ile bazı maya türleri oluşturmaktadır. Bu çalışmada, su kefiri taneleri ile fermente edilen portakal suyuna inoküle edilen E.coli inaktivasyonunun doğrusal (basit doğrusal, Kuadritik ve Kubik) ve doğrusal olmayan modeller (Lojistik, Gompertz ve Brody) ile matematiksel olarak ifade edilmesi ve model uygunluğunun tespit edilmesi amaçlanmıştır. Bu amaçla buzdolabı koşullarında gerçekleştirilen 4 günlük depolama süresi boyunca, E.coli düzeyi kontrol grup (yalnızca portakal suyu) ve su kefiri ile fermente edilen portakal suyu örneklerinde günlük olarak belirlenmiştir. Sonuç olarak, kontrol grup örneklerde 4.82 log kob/mL, fermente portakal suyu örneklerinde ise 5.38 log kob/mL düzeyinde bir E.coli inaktivasyonu gerçekleştiği tespit edilmiştir. Kullanılan modellerin hesaplanan determinasyon katsayıları (R2) ve hata kareler ortalamaları (HKO) baz alınarak model karşılaştırılması yapıldığında, E.coli inaktivasyonunu 0.998 R2 ve 0.025 HKO değerleri ile doğrusal modellerden kübik modelin; 0.995 R2 ve 0.053 HKO değerleri ile doğrusal olmayan modellerden ise Brody modelin en uygun model olduğu tespit edilmiştir.

Anahtar Kelimeler

Su kefiri, portakal suyu, Escherichia coli, matematiksel modelleme

Kaynakça

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