Fındık (Corylus avellana L.) Yeşil Kabuk ve Yaprak Ekstraklarında Biyolojik Aktivite Tayini

Yıl 2016, Cilt: 19 Sayı: 4, 373 - 378, 05.11.2016

Mehmet Özaslan , Sibel Bayıl Oğuzkan Serpil Uğraş Merve Can Ayşe Uzun Sultan Ülger Şebnem Üzmez Bora Karagül İ. Halil Kılıç Halil İbrahim Uğraş

Öz

Türkiye’de özellikle Karadeniz bölgesinde yetişen Corylus avellana L. (C. avellana L.) türü fındıktan yılda 10,000 tondan daha fazla fındık yaprağı ve 300 bin tondan fazla da yeşil kabuk artığı açığa çıkmaktadır. Bu nedenle bu çalışmamızda fındık yeşil yaprak ve kabuğunun biyolojik aktivitelerini belirlemek istedik. Bu çalışmada, fındık yeşil kabuğu ve yaprağı metanol ile oda sıcaklığında uygun optimizasyon koşullarında 72 saat muamele edilerek ektraktları elde edilmiştir. Elde edilen ekstraklardan antibakteriyal aktivite testleri 8 farklı standart bakteri suşu kullanılarak agar kuyu difüzyon yöntemi kullanılarak analiz edilmiştir. Ekstraklarda antiradikalik aktivite tayini için DPPH (1,1-difenil-2-pikrilhidrazil) yöntemi kullanılmıştır. Bu çalışmada ekstrakların H₂O₂ ve UV-C’ye maruz bırakılarak oluşan DNA hasarı üzerine etkileri pBR322 plazmid DNA kullanılarak araştırılmıştır. Çalışmanın sonucunda fındık yeşil yaprak ve kabuklarının antiradikalik aktivite gösterdikleri tespit edilmiş olup ayrıca bu ekstrakların birçok bakteriye karşı antibakteriyel özellik gösterdikleri de bulunmuştur. Fındığın yeşil kabuk ekstaksiyonunun DNA üzerine UV-C ve H₂O₂ ‘in etkilerine karşı koruyucu potansiyeli olduğu fakat yeşil yaprağın DNA koruyucu etki göstermediği tespit edilmiştir. 

Anahtar Kelimeler : Corylus avellana L., DPPH, ekstrakt.

 

Biological Activity Analysis of Hazelnut (Corylus avellana L.) Green Shell and Leaf Extracts

 

ABSTRACT: Corylus avellana L., a nut cultivar indigenous to Black Sea region of Turkey, is able to relese more than 10.000 tons of nut leaf and 300.000 tons of green nut Shell annually. Objective of this study was to determine biologic activities of nut leaf and green Shell. In this study, to attain their extracts, green nut shell and nut leaf were treated with methanol in room temperature for 72 hours. Antibacterial activity of extracted substances were analyzed via eight different bacterial strains according to agar well diffusion method. The DPPH (1,1-diphenyl-2-pikrilhydrazyl) method was used for determining the antiradical activity. In this study, the DNA productive activity of extracts were determined by the DNA of the pBR322 plasmid at the end of exposing them to H₂O₂ and UV-C. As a result of the study, it was determined that hazelnut green leaf and shell showed antiradical activity and antibacterial activities to lots of bacteria. Hazelnut green shell demonstrated a protective potential on DNA against to UV-C and H₂O_2, but this activity was not the case for hazelnut green leaf. 

Key words: Corylus avellana, DPPH, extract. 

Kaynakça

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