SPERM DNA DAMAGE ANALYSIS AMONG INFERTILE MALES WITH NORMAL SEMEN PARAMETERS AND A HISTORY OF ASSISTED REPRODUCTIVE TECHNIQUE FAILURE

Yıl 2018, Cilt: 20 Sayı: 2, 177 - 183, 31.08.2018

Kanay Yararbaş

https://doi.org/10.24938/kutfd.385884

Öz

Objective: This study aims to document the sperm DNA damage results in infertile male patients with normal-standard semen parameters retrospectively.

Material and Methods: The study included samples from 500 male patients with a referred diagnosis of idiopathic infertility and a medical history of at least one assisted reproductive technique (ART) failure. All the patients included in this study were otherwise healthy individuals and had normal-standard semen parameters. We performed retrospective analysis, from the results obtained from samples. Tunel methodology was used, and fluorescence microscopy analysis was performed. From a morphological point of view, apparently normal mature sperms were analyzed.  

Results: Increased sperm DNA damage rates were detected in our tested population. Advanced age did not correlate with a significant increase in sperm DNA damage ratios in our cohort.

Conclusion: The importance of sperm DNA damage should be considered as an etiological factor when evaluating infertile couples. Because the ratio of DNA damage might correlate with various indicators of fertility such as the fertilization rate, embryo cleavage rate, implantation rate, pregnancy rate and live birth rate, it is important to know the ratio of spermatozoa with damaged DNA in the ejaculate in order to predict the fertilization rate and determine the risks that may affect the embryo.

Anahtar Kelimeler

sperm, sperm, Sperm DNA damage, male infertility, sperm

Normal Sperm Parametrelerine Sahip, Yardımcı Üreme Yöntemi Başarısızlığı Öyküsü Bulunan İnfertil Erkeklerde Sperm DNA Hasar Analizi

Öz

Amaç: Bu çalışmada retrospektif olarak, normal semen parametrelerine sahip infertil erkeklerde sperm DNA hasarı analizi sonuçlarının dokümantasyonu amaçlanmaktadır.

Gereç ve Yöntemler: Bu çalışmaya idiopatik infertilite olarak ele alınan ve takip edilen, ek olarak en az bir yardımcı üreme yöntemi başarısızlığı öyküsü bulunan 500 erkek hasta dahil edilmiştir. Tüm olgular, diğer açılardan sağlıklı, normal spermiyogram sonuçlarına sahip hastalar arasından seçilmiştir. Çalışmada Tunel yöntemi ile teste alınıp, floresan mikroskopi ile incelenen hasta örneklerindeki normal görünümlü spermlerde elde edilen sonuçlar dokumante edilmiştir.

Bulgular: Test edilen hasta grubunda artmış sperm DNA hasarı oranları gözlenmiştir. Bulguların yaştan bağımsız dağılım gösterdiği tespit edilmiştir.

Sonuç: İnfertil çift değerlendirmelerinde artmış sperm DNA hasarı da önemli bir parametre olarak göz önünde bulundurulmalıdır. Sperm DNA hasarının varlığı ve oranı, fertilizasyon düzeyi, embriyo yarıklanma oranı, implantasyon başarısı, gebelik ve canlı doğum oranı ile ilişkili olabilir. Bu nedenle sperm DNA hasarı analizi, bu çiftlerde prognoz tayini ve embriyonun sağkalımı ile riskleri konusunda fikir vermesi açısından önem arz etmektedir.

Anahtar Kelimeler

sperm, Sperm DNA hasarı, erkek infertilitesi, sperm

Kaynakça

• 1. Duncan, M. Fecundity, Fertility, Sterility and Allied Topics. Black, Edinburgh, Scotland; 1871.
• 2. Forti G, Krausz C. Evaluation and Treatment of the Infertile Couple. J Clin Endocrinol Met. 1998;83(12):4177-4188.
• 3. Iammarrone E, Balet R, Lower AM, Gillott C, Grudzinskas JG. Male Infertility. Best Pract Res Clin Obstet Gynaecol. 2003;17(2):211-229.
• 4. Guzick DS, Overstreet JW, Factor-Litwak P, Brazil CK, Nakajima ST, Coutifaris C et al. Sperm Morphology, Motility and Concentration In Fertile and Infertile Men. New Engl J Med. 2000;345:1388-1393.
• 5. Zini A, Bielecki R, Phang D, Zenzes MT. Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril. 2001;75:674-7.
• 6. Evenson DP, Jost LK, Marshall D, Zinaman MJ, Clegg E, Purvis K et al. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod. 1999;14:1039-49.
• 7. Spano M, Bonde JP, Hjollund HI, Kolstad HA, Cordelli E, Leter G. Sperm chromatin damage impairs human fertility. The Danish first pregnancy planner study team. Fertil Steril 2000;73:43-50.
• 8. Aydos OS, Yukselten Y, Kaplan F, Sunguroglu A, Aydos K. Analysis of the correlation between sperm DNA integrity and conventional semen parameters in infertile men. Turk J Urol. 2015;41(4):191-7
• 9. Evgeni E, Lymberopoulos G, Gazouli M, Asimakopoulos B. Conventional semen parameters and DNA fragmentation in relation to fertility status in a Greek population. Eur J Obstet Gynecol Reprod Biol. 2015;188:17-23.
• 10. Wyrobek AJ, Eskenazi B, Young S, Arnheim N, Tiemann-Boege I, Jabs EW et al. Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm. P Natl Acad Sci USA. 2006;103:9601-6.
• 11. Aitken RJ, Curry BJ. Redox regulation of human sperm function: from the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line. Antioxid Redox Signal. 2011;14:367-81.
• 12. Martínez-Pastor F, Aisen E, Fernández-Santos MR, Esteso MC, Maroto-Morales A, Garcia-Alvarez O et al. Reactive oxygen species generators affect quality parameters and apoptosis markers differently in red deer spermatozoa. Reprod. 2009;137:225-35.
• 13. Gazo I, Shaliutina-Kolešová A, Dietrich MA, Linhartová P, Shaliutina O, Cosson J. The effect of reactive oxygen species on motility parameters, DNA integrity, tyrosine phosphorylation and phosphatase activity of common carp (Cyprinus carpio L.) spermatozoa. Mol Reprod Dev. 2015;82:48-57.
• 14. Host E, Lindenberg S, Smidt-Jensen S. The role of DNA strand breaks in human spermatozoa used for IVF and ICSI. Acta Obstet Gynecol Scand. 2000;79:559-63.
• 15. Lopes SM, Sun JG, Jurisicova A, Meriano JB, Casper RF. Sperm deoxyribonucleic acid fragmentation is increased in poor-quality semen samples and correlates with failed fertilization in intracytoplasmic sperm injection. Fertil Steril. 1998;69:528-32.
• 16. Ahmadi A, Ng SC. Fertilizing ability of DNA-damaged spermatozoa. J Exp Zool. 1999; 284:696-704.