Biberiye bitkisine ait uçucu yağın in vitro koşullarda Alternaria brassicicola etmenine karşı etkilerinin belirlenmesi

Yıl 2022, Cilt: 2 Sayı: 1, 1 - 8, 30.06.2022

Haco Aydın , Nedim Altın

Öz

Alternaria brassicicola lahanagiller familyasına ait bitkilerde hastalığa neden olan en önemli patojenlerden birisidir. Bu etmene karşı kültürel önlemler, kimyasal ve biyolojik mücadele yöntemleri kullanılmaktadır. Bitki uçucu yağlarının bitkilerde hastalık oluşturan fungal etmenlere karşı etkileri üzerine çeşitli araştırmalar yapılmaktadır. Bu çalışma biberiye bitkisine ait uçucu yağın A. brassicicola etmenine karşı antifungal etkilerini belirlemek amacıyla in vitro koşullarda yürütülmüştür. Clevenger cihazında hidrodistilasyon yöntemi kullanılarak uçucu yağ elde edilmiştir. Uçucu yağın A. brassicicola etmenine karşı antifungal etkileri 10 µl/20ml PDA, 20 µl/20ml PDA, 40 µl/20ml PDA, 80 µl/20ml PDA, 160 µl/20ml PDA ve 320 µl/20ml PDA kosantrasyonlarda denenmiştir. Elde edilen sonuçlara göre biberiye uçucu yağı 80 µl/20ml PDA ve üzerindeki dozlarda güçlü antifungal etki göstermiştir. Ancak koparılmış yaprak denemelerinde tüm dozlarda etki elde edilememiştir.

Anahtar Kelimeler

antifungal, bitki patojeni, medicinal and aromatic plant

Destekleyen Kurum

yok

Proje Numarası

yok

Determination of the effect of essential oil of rosemary plant against Alternaria brassicicola in in vitro conditions

Öz

Alternaria brassicicola is one of the most important pathogens that cause disease in plants belonging to the cabbage family. Cultural measures, chemical and biological control methods are used against this pathogen. Many studies have been conducted on the effects of plant essential oils against fungal pathogens that cause disease in plants. This study was carried out to determine the antifungal effects of rosemary essential oil against A. brassicicola in in vitro conditions. The essential oil was obtained by using the hydrodistillation method in the Clevenger apparatus. The antifungal effects of essential oil against A. brassicicola were tested at concentrations of 10 µl/20ml PDA, 20 µl/20ml PDA, 40 µl/20ml PDA, 80 µl/20ml PDA, 160 µl/20ml PDA and 320 µl/20ml PDA. According to the results, rosemary essential oil showed a strong antifungal effect at doses of 80 µl/20ml PDA and above. However, no effect was obtained at all doses in the detached leaf trials.

Anahtar Kelimeler

antifungal, plant pathogen, medicinal and aromatic plant

Proje Numarası

yok

Kaynakça

• Barkaoui H, Chafik Z, Benbbas R, Chetouani M, El Mimouni M, Hariri E (2022). Antifungal activity of the essential oils of Rosmarinus officinalis, Salvia officinalis, Lavandula dentata and Cymbopogon citratus against the mycelial growth of Fusarium oxysporum f.sp. albedinis Arabian Journal of Medicinal & Aromatic Plants 8(1): 108-133
• Bozhüyük A U, Kordali Ş, Bölük G (2015). Satureja hortensis L. uçucu yağının antifungal etkisi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 46(2): 107-112
• Fontenelle A D B, Guzzo S D, Lucon C M M, Harakava R, (2011). Growth promotion and induction of resistance in tomato plant against Xanthomonas euvesicatoria and Alternaria solani by Trichoderma spp. Crop Protection 30: 1492-500
• Humpherson-Jones F M (1989). Survival of Alternaria brassicae and Alternaria brassicicola on crop debris of oilseed rape and cabbage. Annals of Applied Biology 115: 45-50
• Kadium S W, Khalil A M, Ammar Semysim E A R (2021). Antifungal Activity Of Rosmarinus officinalis and Pelargonium gravelens essential oils extracts against Aspergillus flavus, Penicillium brachycaulon and Alternaria alternate. Natural Volatiles & Essential Oils 8(4): 3498-3509
• Lawrence C B, Mitchell T K, Craven K D, Cho Y, Cramer R A, Kim K H, (2008). At death's door: Alternaria pathogenicity mechanisms. Plant Pathology Journal 24: 101-111
• Lorenzetti E, Stangarlin J R, Kuhn O J (2017). Antıfungal activity of rosemary extract on Macrophomına phaseolına and charcoal rot control in soybean. Journal of Plant Pathology 99(3): 783-786
• Nowicki M, Nowakowska M, Niezgoda A, Kozik E U, (2012). Alternaria black spot of crucifers: symptoms, importance of disease, and perspectives of resistance breeding. Vegetable Crops Research Bulletin 76: 5–19
• Omar MS., Kordali Ş (2019). Review of essential oils as antifungal agents for plant fungal diseases. Ziraat Fakültesi Dergisi 14(2): 294-301
• Seaman A, (2012). Production Guide for Organic Cole Crops: Cabbage, Cauliflower, Broccoli, and Brussels Sprouts. Geneva, NY: NYS IPM.
• Sivapalan A, (1993). Fungi associated with broccoli seed and evaluation of fungal antagonists and fungicides for the control of seed-borne Alternaria brassicicola. Seed Science and Technology 21: 237-45.
• Türkkan M, Çalışkan Ö, Erper İ, Kara Ş M, Açıkgöz M A (2019). Bazı toprak kökenli funguslara karşı defne esansiyel yağı ve hidrosölünün antifungal etkilerinin belirlenmesi. Akademik Ziraat Dergisi 8(2): 217-226
• Üstüner T, Kordali S, Bozhüyük A U (2018). Herbicidal and fungicidal effects of Cuminum cyminum, Mentha longifolia and Allium sativum essential oils on some weeds and fungi. Records of Natural Products 12(6): 619.