The Investigation of Mutagenic Effect of Cinamon (Cinnamomum zeylanicum) By Ames Test

Yıl 2015, Cilt: 8 Sayı: 2, 23 - 27, 01.12.2015

Fahriye Zemheri

Öz

Today in the world the essential oils have a wide area of usage from cosmetics and perfume, industry to food and pharmaceutical industry. Knowing the wide usage area of mutagenic and antimutagenic effect of this kind of plant may have crucial importance in the treatment of a variety of illnesses and cancer. In this study, the mutagenic effect of the essential oil which is obtained from cinnamon (Cinnamomum zeylanicum) has been investigated. Aiming this, in this experiment Ames mutagenite test, which is widely used, has been applied. Various doses of oil’s mutegenic effect have been investigated in the TA98 and TA100 strains of Salmonella typhimirium. As a result it is appointed that TA98 strain of Salmonella typhimirium shows toxic in14 μg/ml and TA100 strain of Salmonella typhimirium shows low mutagenic effect in 4 ve 2 μg/ml

Anahtar Kelimeler

Ames Test, Mutagenicity, Salmonella typhimirium, Cinamon (Cinnamomum zeylanicum)

Tarçının (Cinnamomum zeylanicum) Ames Testi ile Mutajenik Etkisinin Araştırılması

Öz

Günümüzde uçucu yağların kozmetik, parfüm ve gıda sanayinden, ilaç sanayine kadar geniş bir kullanım alanı vardır. Bu şekilde geniş kullanım alanına sahip olan bitkilerin mutajenik ve antimutajenik etkisini belirlemek çeşitli hastalıklar ve kanserin tedavisinde büyük öneme sahiptir. Bu çalışmada tarçın (Cinnamomum zeylanicum) bitkisinden elde edilen uçucu yağın mutajenik etkisi, yaygın olarak kullanılan Ames mutajenite test yöntemi kullanılarak araştırılmıştır. Çeşitli dozlarda kullanılan yağın mutajenik etkisi Salmonella typhimirium’ un TA98 ve TA100 suşlarında araştırılmış ve sonuç olarak Salmonella typhimirium’ un TA98 suşunda 14 μg/ml’de toksik olduğu, TA100 suşunda 4 ve 2 μg/ml’de zayıf mutajenik etki gösterdiği saptanmıştır.

Anahtar Kelimeler

Ames Test, Mutajenik Etki, Salmonella typhimirium, Tarçın (Cinnamomum zeylanicum)

Kaynakça

• Bruneton J. Pharmacognosy, Phytochemistry, Medicinal Plants. Lavoisier matbaası, Paris. 1995; 509.
• Deletre E, Chandre F, Williams L, Duménil C, Menut C, Martin T. Electrophysiological and behavioral characterization of bioactive compounds of the Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum and Cinnamomum zeylanicum essential oils against Anopheles gambiae and prospects for their use as bednet treatments. Parasites & Vectors 2015; 8-316.
• Demirsoy A.Kalıtım ve Evrim, Meteksan, Ankara.2005
• Dığrak M, İlçim A, Alma MH. Antimicrobial activities of several parts of Pinus brutia, Juniperus oxycedrus, Abies cilicia, Cedrus libani and Pinus nigra. Phytotherapy Research. 1999; 13: 584-587.
• Eren Y, Özata A. Determination of mutagenic and cytotoxic effects of Limonium globuliferum aqueous extracts by Allium, Ames, and MTT tests. Rev Bras Farmacogn 24 2014; 51-59.
• Essawi T, Srour M. Screening of some Palestinian medicinal plants for antibacterial activity. J. Ethnopharmacol. 2000; 70: 343- 349.
• Forman D, Ames B. The Ames Test and the Causes of Cancer. Br. Med. J. 1991; 303: 428-429.
• Guynot ME, Ramos AJ, Seto L, Purroy P, Sanchis V, Marin S. Antifungal activity of volatile compounds generated by essential oils against fungi commonly causing deterioration of bakery products. J. Appl. Microbiol. 2003; 94(5): 893–899.
• Jayaprakasha G K, Negi P S, Jena B S, Rao L J M.Antioxidant and antimutagenic activities of Cinnamomum zeylanicum fruit extracts. Journal of Food Composition and Analysis 20, 2007; 330–336.
• Kayraldız A. Sodyum Metabisülfit’in sıçan kemik iliği hücrelerinde in vivo genotoksik etkileri, Doktora Tezi, Ç.Ü. Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Adana 2005.
• Klug WS, Cummings MR. Concept of Genetics, 6 th Edition, 0-13-081626-4 (Çev. ÖNER, C. Genetik Kavramlar, Palme Yayıncılık. 2002; 816.
• Khan A, Safdar M, Khan MMA, Khattak KN, Anderson RA. Cinnamon Improves Glucose and Lipids of People With Type 2 Diabetes. Diabetes Care. 2002; 26:3215-3218.
• Karnick CR. Pharmacopoeial Standards of Herbal Plants. Sri Satguru matbaası, Delhi. 1994; 1138.
• Kim SH, Hyun SH, Choung SY. Anti-diabetic effect of cinnamon extract on blood glucose in db/db mice. Journal of Ethnopharmacology. 2005; 18:3953-3958.
• Lopez P, Sanchez C, Batlle R, Nerin C. Solid- and vapour-phase antimicrobial activities of six essential oils:susceptibility of selected foodbourne bacterial and fungal strains. J Agri Food Chem. 2005; 53(17):6939-46.
• Maksimović ZA, Dordević S, Mraović M. Antimicrobial activity of Chenopodium botrys essential oil. Fitoterapia. 2005; 76: 112-114.
• Maron D, Ames BN. Revised Methods For Salmonella Mutagenicity Test, Mutat. Res. 1983; 113, 173-215.
• Nagao M, Honda M, Seıno Y, Yahagi T, Kawachi T. Mutagenicities of protein pyrolysates. Cancer Lett. 1977; 2(6), 335-339.
• Özer Z, Tursun N, Önen H. Yabancı Otlarla Sağlıklı Yaşam (Gıda ve Tedavi). Ankara. 4Renk Yayınları. 2001; 133.
• Shan B, Cai YZ, Sun M, Corke H. Antioxidant capacity of 26 spices extracts and characterization of their phenolic constituents. J Agric Food Chem, 2005; 53(20):7749-59.
• Synder L, Champness W. Third Edition Moleküler Genetics of Bacteria, Department of Microbiology and Moleculer Genetics, Michigon State University East Lansing, Michigan Washigton, DC. 2007; 492.
• Tüylü B. Bazı İlaç Öncül Maddelerinin Mutajenik Etkilerinin Bakteriyal ve Hücre Kültürü Testleri İle Araştırılması, Doktora Tezi, Anadolu Üniversitesi Fen Fakültesi Biyoloji Ana Bilim Dalı 2001; 15.
• Unlu M, Ergene E, Vardar Unlu G, Sivas Zeytinoğlu H, Vural N. Composition, antimicrobial activity and in vitro cytotoxicity of essential oil from Cinnamomum zeylanicum Blume (Lauraceae). Food and Chemical Toxicology 48 2010; 3274-3280.
• Yıldırım A, Bardakçı F, Karakaş M, Tanyolaç B. Moleküler Biyoloji ;Nobel Yayın Dağıtım; 2007.
• Verspohl EJ, Bauer K, Neddermann E. Antidiabetic effect of Cinnamomum cassia and Cinnamomum zeylanicum in vivo and in vitro. Phytother Res. 2005; 19(3):203-6.
• Vetal S, Bodhankar SL, Mohan V, Thakurdesai P A. Anti-inflammatory and anti-arthritic activity of type-A procyanidine polyphenols from bark of Cinnamomum zeylanicum in rats. Food Science and Human Wellness. 2, 2013; 59–67.
• Zeytinoğlu H, Ergene E, Tüylü B. Bazı (Kloro-Fenil) Fenantroimidazol Türevlerinin Mutajenik Aktivitesinin Ames/Salmonella/Mikrozom Testi İle Araştırılması, Anadolu Üniversitesi Fen Fakültesi Biyoloji Bölümü (79-80) XIV. Ulusal Biyoloji Kongresi 7-10 Eylül 1998, SAMSUN Cilt III, 1998; 78-89.