Pathologic Examination of the Protective Effect of Glycyrrhizin on Cyclophosphamide-induced Hemorrhagic Cystitis in Rats

Yıl 2019, Cilt: 12 Sayı: 1, 89 - 96, 31.03.2019

Okan Erdogan Hikmet Keles

https://doi.org/10.30607/kvj.515979

Cited By: 2

Öz

Cyclophosphamide (CP) is a
widely used anti-neoplastic agent in humans and small animals and also has some
adverse effects in them. The most common adverse effect of CP is hemorrhagic
cystitis (HS). The use of Mesna may prevent HS, but is insufficient for some
side effects. In this study, the effect of glycyrrhizin (GLY), which is one of the active substances of licorice
root, was examined against CP-related
side effects. The experiment was designed with 49 male Sprague Dawley
rats. The Control group received only physiological saline and the CP group
only CP. CP+Mesna group received CP and also three dose of Mesna. The animals
in the CP+GLY100 and CP+GLY200 groups were received three doses of GLY, 100
mg/kg and 200 mg/kg dose, respectively. The previous protocol was applied to
the CP+Mesna+GLY100 and CP+Mesna+GLY200 groups and only the first GLY
application was replaced with Mesna. In this study, it was recorded that
bladder macroscopy and histopathology were better preserved in GLY applicated
groups compared to CP group. However, it was observed that this protection was
slightly weaker than the Mesna and GLY-Mesna combinations. From this point of
view, it was observed that GLY could be preventive in CP-related HS, in
addition it could also support to Mesna applications.

Anahtar Kelimeler

Cyclophosphamide, Glycyrrhizin, Hemorrhagic cystitis, Mesna, Pathology

Ratlarda Siklofosfamid ile İndüklenen Hemorajik Sistitisde Glycyrrhizinin Koruyucu Etkilerinin Patolojik Olarak İncelenmesi

Öz

Siklofosfamid (CP) yaygın olarak kullanılan bir
anti-neoplastik ajandır ve bazı yan etkilere sahiptir. Siklofosfamidin en
belirgin yan etkisi hemorajik sistitistir (HS). Mesna kullanımı HS’yi
önleyebilmekte ancak diğer bazı yan etkiler için yetersiz kalmaktadır. Bu
çalışmada, CP ilişkili HS’ye karşı, meyan kökünün aktif içeriklerinden olan
glycyrrhizinin (GLY) etkisi incelenmiştir. Çalışma, 49 adet, erkek Sprague
Dawley rat ile dizayn edilmiştir. Kontrol grubuna sadece fizyolojik tuzlu su,
CP grubuna ise sadece CP verilmiştir. CP+Mesna grubuna CP ve ayrıca 3 doz Mesna
uygulanmıştır. CP+GLY100 ve CP+GLY200 gruplarına; CP ve sırasıyla 100 mg/kg ile
200 mg/kg olmak üzere üç doz GLY uygulanmıştır. Bir önceki protokol
CP+Mesna+GLY100 ve CP+Mesna+GLY200 gruplarınada uygulanmış olup sadece ilk GLY
uygulaması Mesna ile değiştirilmiştir. Çalışmada GLY uygulanan gruplarda CP
grubuna kıyasla mesane makroskobisi ve histopatolojisinin daha iyi bir şekilde
korunmuş olduğu kaydedilmiştir. Ancak, bu korumanın Mesna ve GLY-Mesna
kombinasyonlarına kıyasla biraz daha zayıf kaldığı izlenmiştir. Buradan yola
çıkılarak GLY’in CP ilişkili HS’de önleyici olabileceği aynı zamanda Mesna
uygulamalarına destek verebileceği kanaatine varılmıştır.

Anahtar Kelimeler

Glycyrrhizin, Hemorajik sistitis, Mesna, Patoloji, Siklofosfamid

Kaynakça

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