Morphometric and Stereological Assessment of The Bovine Vesicular Gland

Yıl 2020, Cilt: 13 Sayı: 3, 254 - 261, 30.09.2020

Murat Sirri Akosman , Vural Özdemir

Öz

The vesicular gland is an important genital gland related to the fertility of bovine. In this study, the morphometric and stereological methods were sought to adapt to the bovine vesicular gland. For this aim, 8 healthy Holstein bull’s paired vesicular glands were collected from the slaughterhouse. The weight and the dimensions of the glands were measured before the fixation. After the formalin fixation, all the glands were divided into 3 subgroups for the total volume estimation of the gland, cell counting and the volume estimation of the cell nucleus. The cell counting and the volume estimation of the cell nucleus were performed on the principal cells. The average weight, width, length, height, the volume of the glands, the mean number and nucleus volume of the principal cells were 30.9 g., 2.62 cm, 8.86 cm and 1.86 cm, 29.7 cm3, 3.58.109 and 108 µm3, respectively. The researchers believe that the findings will contribute to the literature and in particular, facilitate experimental and toxicological researches performed in the future.

Anahtar Kelimeler

Cell number, Nucleus volume, Optical fractionator, Stereology, Vesicular gland

Destekleyen Kurum

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Proje Numarası

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Teşekkür

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Sığır Veziküler Bezinin Morfometrik ve Stereolojik Olarak Değerlendirilmesi

Öz

Veziküler bez sığır fertilitesinde önemli bir üreme bezidir. Sunulan bu çalışmada sığır veziküler bezinin morfometrik ve stereolojik olarak incelenmesi amaçlanmıştır. Bu amaçla 8 sağlıklı Holstein boğasının çift olan veziküler bezleri mezbahadan toplanmış ve ağırlığı ile boyutları ölçülmüştür. Formaldehit tespitinden sonra tüm bezler bezin toplam hacminin, hücre sayısının ve çekirdek hacminin ölçülmesi için 3 alt gruba ayrılmıştır. Hücre sayımı ve hücre çekirdek hacmi ölçümü bezin en çok bulunan ana hücresi olan principal hücreler üzerinde gerçekleştirilmiştir. Bezin ortalama ağırlığı, eni, uzunluğu, yüksekliği, ortalama hacmi, principal hücrelerin ortalama sayısı ve ortalama çekirdek hacmi sırasıyla 30,9 gr., 2,62 cm, 8,86 cm, 1,86 cm, 29,7 cm3, 3,58,109 ve 108 µm3 olarak bulunmuştur. Araştırmacılar bu bulguların literature katkı sağlayacağına ve gelecekte yapılacak olan deneysel ve toksikolojik çalışmalara ışık tutacağına inanmaktadır.

Proje Numarası

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Kaynakça

• Amselgruber W, Feder FH. Licht- und elektronenmikroskopische untersuchungen der samenblasendrüse (glandula vesicularis) des bullen. Anat. Histol. Embryol. 1986;15:361-379.
• Archana P, Katiyar RS, Sharma DN, Farooqui MM. Gerentological studies on the gross and histomorphology of the vesicular gland of gaddi goat (capra hircus). Int. J. Morphol. 2009;27(1):13-20.
• Aughey E, Frye FL. Comparative veterinary histology with clinical correlates. Iowa State University Press, Iowa. USA. pp.175. 2001.
• Baddeley AJ, Gundersen HJG, Cruz-Orive LM. Estimation of surface area from vertical sections. J. Microsc. 1986;142(3):259-276.
• Badia E, Briz MD, Pinart E, Sancho S, Garcia N, Bassols J, Pruneda A, Bussalleu E, Yeste M, Casas I, Bonet S. Structural and ultrastructural features of boar seminal vesicles. Tissue and Cell. 2006;38:7-18.
• Budras KD, Habel RE, Wünsche A, Buda S. Bovine anatomy, An illustrated text. Schlütersche GmbH, Hannover, Germany. pp. 92. 2003.
• Chandolia RK, Honaramooz A, Omeke BC, Pierson R, Beard AP, Rawlings NC. Assessment of development of the testes and accessory glands by ultrasonography in bull calves and associated endocrine changes. Therio. 1997;48:119-132.
• Cons, DN. Some observations on the histology and histochemistry of the seminal vesicles of the bull. J. Endocrin. 1957;14:304-308.
• Dorph-Petersen KA, Nyengaard JR, Gundersen HJG. Tissue shrinkage and unbiased stereological estimation of particle number and size. J. Microsc. 2001;204(3):232-246.
• Lodrup AB, Karstoft K, Dissing TH, Pedersen M, Nyengaard JR. Kidney biopsies can be used for estimations of glomerular number and volume: A pig study. Virchows Arch. 2008;452: 393-403.
• Frandson RD, Wilke L, Fails AD. Anatomy and physiology of farm animals. State Avenue, Ames, Iowa, USA. pp. 375. 2003.
• Gartner LP, Hiatt JL. Color textbook of histology. Saunders. pp. 505. 2006.
• Gomes IC, Cagnon VHA, Carvalho CAF, De Luca IMS. Stereology and ultrastructure of the seminal vesicle of c57/bl/6j mice following chronic alcohol ingestion. Tissue and Cell. 2002;4(3):177–186.
• Gundersen HJG, Bendtsen TF, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A, West MJ. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. Apmis. 1988;96:379-394.
• Gundersen HJG, Jensen EBV, Kieu K, Nielsen J. The efficiency of systematic sampling in stereology- reconsidered. J. Microsc. 1999;193(3):199-211.
• Gundersen HJG. The smooth fractionator. J. Microsc. 2002;207(3):191-210.
• Macmillan KL, Hafs HD. Reproductive tract of holstein bulls from birth trough puberty. J. Anim. Sci. 1969;28:233-239.
• Marettova E, Legath J. The presence of smooth muscle cells and elastic fibers in the bull vesicular gland. Slov. Vet. Res. 2010;47(3):91-96.
• Michel PR, Cruz-Orive LM. Application of the cavalieri principle and vertical sections method to lung: estimation of volume and pleural surface area. J. Microsc. 1998;150(2):117-136.
• Møller A, Strange P, Gundersen HJG. Efficient estimation of cell volume and number using the nucleator and the disector. J. Microsc. 1990;159(1):61-71.
• Oguz EO, Conkur ES, Sarı M. Shtereom I simple windows based software for stereology, volume and number estimations. Image Anal. Stereol. 2007;26:45-50.
• Pakurar AS, Bigbee JW. Digital Histology. John Wiley & Sons, Inc., Hoboken, New Jersey. USA. pp. 190-191. 2004.
• Rahman MS, Islam MS, Rahman MT, Parvez NH, Rhaman MM. Morphometric analysis of vesicular glands of indigenous bull. Int. J. Sustain. Crop. Prod. 2010;5(1):11-14.
• Santos M, Marcos R, Santos N. Malhao F, Monteiro RAF, Rocha E. An unbiased stereological study on subpopulations of rat liver macrophages and on their numerical relation with the hepatocytes and stellate cells. J. Anat. 2009;214(5):744-751.
• Sørensen FB. Objective histopathologic grading of cutaneous malignant melanomas by stereologic estimation of nuclear volume. Cancer. 1989;63:1784-1798.
• Sørensen FB. Stereological estimation of the mean and variance of nuclear volume from vertical sections. J. Microsc. 1991;162(2):203–229.
• West MJ, Slomianka L, Gundersen HJG. Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator. Anat. Rec. 1991;231(4):482-497.