Enerjilendirilmiş Oksijen Molekülleri İçeren Bitkisel İçerikli Ürünün Yara İyileşmesi Üzerindeki Etkinliğinin Değerlendirilmesi

Yıl 2023, Cilt: 16 Sayı: 2, 195 - 208, 30.06.2023

Nurullah Okumuş , Sevim Feyza Erdoğmuş , Özlem Erdal Altıntaş , Hasan Hüseyin Demirel , Sefa Çelik

Öz

Son yıllarda, tıbbi ve aromatik bitkilerin kullanımıyla ilgili ileri formülasyon teknolojilerinin gelişmesi ile birlikte bitkisel içerikli medikal ürünlere olan ilgi giderek artmaktadır. Geliştirilen bu ürünler, yara ve yanıkların tedavisini hızlı ve etkili bir şekilde gerçekleştirebilmektedir. Bu çalışmada, ilk kez enerjilendirilmiş oksijen molekülü içeren bitkisel içerikli ürünün yara iyileşme üzerindeki etkinliği in vitro ve in vivo çalışmalar ile değerlendirilmiştir. Çalışma kapsamında, Hypericum perforatum L. tıbbi yağı ve enerjilendirilmiş oksijen molekülü içeren bazın biyolojik etkinliği belirlenmiştir. Bu çalışma sonuçları, enerjilendirilmiş oksijen molekülleri içeren bazın ve Hypericum perforatum L. tıbbi yağının patojen test mikroorganizmaları üzerinde antimikrobiyal etkinliğinin bulunduğunu ve insan dermal fibroblast hücreleri üzerinde toksik bir etkisinin bulunmadığını göstermiştir. Enerjilendirilmiş oksijen molekülü içeren bitkisel içerikli ürünün ikinci derece derin yanıklarda etkili olduğu belirlenmiştir. Ürünün yara iyileştirici etkinliğinin antimikrobiyal, antifungal, antioksidan ve hücre proliferasyonunu artırıcı etkisi sayesinde gerçekleştiği düşünülmektedir. İleride yapılacak klinik çalışmalar doğrultusunda ürün ikinci derece yanıkların tadavisinde kullanılabilme potansiyeline sahiptir.

Anahtar Kelimeler

Enerjilendirilmiş oksijen molekülleri, Hypericum perforatum L., Yara iyileşme

Destekleyen Kurum

Afyonkarahisar Sağlık Bilimleri Üniversitesi Bilimsel Araştırma Projeleri Komisyonu

Proje Numarası

19. TEMATİK.002

Evaluation of Effectiveness of the Containing Energized Oxygen Molecules Herbal Product on the Wound Healing

Öz

In recent years, the interest in herbal medicinal products has been increasing with the development of advanced formulation technologies related to the use of the medicinal and aromatic plants. These developed products are used to treat wounds and burns quickly and effectively. In this study, the effectiveness of containing energized oxygen molecules herbal product on wound healing was evaluated by in vitro and in vivo studies for the first time. Within the scope of the study, the biological activities of Hypericum perforatum L. medicinal oil, energized oxygen molecules base were determined. Results of this study showed that the base containing energized oxygen molecules and the medicinal oil of Hypericum perforatum L. had antimicrobial activity on pathogen test microorganisms and did not have a toxic effect on human dermal fibroblast cells. It has been determined that the herbal product containing energized oxygen molecule is effective in second degree deep burns. It is thought that the wound-healing effect of the product is realized thanks to its antimicrobial, antifungal, antioxidant and cell proliferation-increasing effect. In line with future clinical studies, the product has the potential to be used in the treatment of second-degree burns.

Anahtar Kelimeler

Energized oxygen molecules, Hypericum perforatum L., Wound healing

Proje Numarası

19. TEMATİK.002

Kaynakça

• Solomon, D., Adams, J., & Graves, N. (2013). Economic evaluation of St. John's wort (Hypericum perforatum) for the treatment of mild to moderate depression. Journal of affective disorders, 148(2-3), 228-234.
• Burunkaya, B., Selli, S., & Kelebek, H. (2021). Sarı Kantaron (Hypericum perforatum L.) Fenoliklerinin Karakterizasyonu, Antioksidan ve Antimikrobiyal Potansiyelinin Belirlenmesi. Çukurova tarım ve gıda bilimleri dergisi, 36(2), 309-324.
• Clinical and Laboratory Standards Institute (CLSI) 2002. Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard. 2nd ed. Document M27-A2. Wayne, PA: Clinical and Laboratory.
• Clinical and Laboratory Standards Institute (CLSI) 2006. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. 7th ed. Document M7-A7. Wayne, PA: Clinical and Laboratory.
• Clinical and Laboratory Standards Institute (CLSI) 2016. Performance standards for antimicrobial standards institute susceptibility testing, 26 th Edition. CLSI supplement M100S. Wayne, PA: Clinical and Laboratory. Çelen, G., Özkan, S., & Ayhan, F. (2008). The phenolic compounds from Hypericum perforatum and their antimicrobial activities. Hacettepe Journal of Biology and Chemistry, 36(4), 339-345.
• Demirci, T., Hasköylü, M. E., Eroğlu, M. S., Hemberger, J., & Öner, E. T. (2020). Levan-based hydrogels for controlled release of Amphotericin B for dermal local antifungal therapy of Candidiasis. European journal of pharmaceutical sciences, 145, 105255.
• Denizot, F., & Lang, R. (1986). Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of immunological methods, 89(2), 271-277.
• Duman, R., Dogan, H.H., & Tuncer, P. (2017). Evaluation of the in vitro antiviral activity of Salvia halophila and Salvia sclarea extracts against human respiratory syncytial virüs (HRSV). International journal of science and research, 3 (7), 44-59.
• Erdal Altıntaş, Ö., & Erdoğmuş, S. F. (2023). Development of controlled delivery systems by nanoliposomes of Hypericum perforatum L. extracts. International Journal of Plant Based Pharmaceuticals, 3(1), 86–94. https://doi.org/10.29228/ijpbp.20
• Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry, 38(12), 1103-1111.
• Erel, O. (2004). A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical biochemistry, 37(2), 112-119.
• Gamez-Meza, N., Noriega-Rodriguez, J. A., Medina-Juarez, L. A., Ortega-Garcia, J., Cazarez-Casanova, R., & Angulo-Guerrero, O. (1999). Antioxidant activity in soybean oil of extracts from Thompson grape bagasse. Journal of the American Oil Chemists' Society, 76, 1445-1447.
• Hussain A. (2013). Surgical treatment of acute burns. Wounds, 9(4), 54-59.
• Hajialyani, M., Tewari, D., Sobarzo-Sánchez, E., Nabavi, S. M., Farzaei, M. H., & Abdollahi, M. (2018). Natural product-based nanomedicines for wound healing purposes: therapeutic targets and drug delivery systems. International journal of nanomedicine, 13, 5023.
• Keleş, O., Ak, S., Bakirel, T., & Alpinar, K. (2001). Screening of some Turkish plants for antibacterial activity. Turkish journal of veterinary and animal sciences, 25(4), 559-565.
• Kıyan, S., Uyanikgil, Y., Altunci, Y. A., Cavusoglu, T., Cetin Uyanikgil, E. O., & Karabey, F. (2015). Investigation of acute effects of Hypericum perforatum (St. John’s Wort-Kantaron) treatment in experimental thermal burns and comparison with silver sulfadiazine treatment. Ulus travma acil cerrahi dergisi, 21 (5), 323-336.
• Markiewicz-Gospodarek, A., Kozioł, M., Tobiasz, M., Baj, J., Radzikowska-Büchner, E., & Przekora, A. (2022). Burn Wound Healing: Clinical Complications, Medical Care, Treatment, and Dressing Types: The Current State of Knowledge for Clinical Practice. International Journal of Environmental Research and Public Health, 19, 1338.
• Nobakht, S.Z., Akaberi, M., Mohammadpour, A.H., Moghadam, A.T., & Emami, S.A. (2022).  Hypericum perforatum: Traditional uses, clinical trials, and drug interactions. Iranian Journal of Basic Medical Sciences, 25(9), 1045-1058.
• Noor, A., Afzal, A., Masood, R., Khaliq, Z., Ahmad, S., Ahmad, F., Qadir, M.B., & Irfan, M. (2022). Dressings for burn wound: a review. Journal of Material Science, 57, 6536–6572
• O'Brien, S. P., & Billmire, D. A. (2008). Prevention and management of outpatient pediatric burns. Journal of craniofacial surgery, 19(4), 1034-1039.
• Orhan, I. E. (2015). LC-DAD-MS-Assisted quantification of marker compounds in Hypericum perforatum L.(St. John’s wort) and its antioxidant activity. Turkish journal of pharmaceutical sciences, 12(3).
• Özkan, E.E., Çelik, B.Ö., & Afife, M. (2019). Antimicrobial activities of five endemic Hypericum species from Anatolia compared with Hypericum perforatum. Journal of Research Pharmacology, 23, 114-119.Öztürk, N.,
• Korkmaz, S., & Öztürk, Y. (2007). Wound-healing activity of St. John's Wort (Hypericum perforatum L.) on chicken embryonic fibroblasts. Journal of ethnopharmacology, 111(1), 33-39.
• Parin, F.N., & Deveci, S. (2023). Production and Characterization of Bio-based Sponges Reinforced with Hypericum perforatum oil (St. John′s Wort Oil) via Pickering Emulsions for Wound Healing Applications. ChemistrySelect, 8(5), e202203692.
• Patel, P.P., Vasquez, S.A., Granick, M.S., & Rhee, S. T. (2008). Topical antimicrobials in pediatric burn wound management. journal of craniofacial surgery, 19(4), 913-922.
• Raja, K.S., Garcia, M. S., & Isseroff, R.R. (2007). Wound re-epithelialization: modulating keratinocyte migration in wound healing. Frontiers in bioscience-landmark, 12(8), 2849-2868.
• Saddiqe, Z., Naeem, I., & Maimoona, A. (2010). A review of the antibacterial activity of Hypericum perforatum L. Journal of ethnopharmacology, 131(3), 511-521.
• Suha, S.A., & Sanam, T.F. (2022). A deep convolutional neural network-based approach for detecting burn severity from skin burn images. Machine Learning with Applications, 9, 100371,https://doi.org/10.1016/j.mlwa.2022.100371.
• Süntar, I.P., Akkol, E.K., Yılmazer, D., Baykal, T., Kırmızıbekmez, H., Alper, M., & Yeşilada, E. (2010). Investigations on the in vivo wound healing potential of Hypericum perforatum L. Journal of ethnopharmacology, 127(2), 468-477.
• Tecer LH., & Gündüz AM. (2021). Design of a new cold atmospheric plasma reactor based on dielelectric barrier discharge for the treatment and recovery of textile dyeing wastewater: Profoks/CAP Reactor. Sustainable practices in the textile ındustry, 285-305. https://doi.org/10.1002/9781119818915.ch12
• Tolkunova, N.N., Cheuva, E.N., & Bidyuk, A.Y. (2002). Effect of medicinal plant extracts on microorganism development. Pishchevaya promyshlennost, 8, 70-71.
• Townsend, C.M., Beauchamp, R.D., Evers, B.M., & Mattox, K.L. (2016). Sabiston textbook of surgery. Elsevier Health Sciences.
• Velnar, T., Bailey, T., & Smrkolj, V. (2009). The wound healing process: an overview of the cellular and molecular mechanisms. Journal of international medical research, 37(5), 1528-1542.
• Yalcınkaya, E., Basaran, M.M., Tunckasık, M.E., Yazici, G.N., Elmas, Ç., & Kocaturk, S. (2022). Efficiency of hypericum perforatum, povidone iodine, tincture benzoin and tretinoin on wound healing, Food and Chemical Toxicology, 166, 113209, https://doi.org/10.1016/j.fct.2022.113209.