Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey

Yıl 2019, Cilt: 59 Sayı: 2, 72 - 78, 13.12.2019

Sena Ardıçlı , Hakan Ustüner Öznur Arslan Oğuz Kandazoğlu

Öz

The objective of the present study was to determine genotypic/allelic frequencies and population genetic indices of CAPN1 g.5709 C>G and MYF5 g.1911 A>G polymorphisms in beef cattle imported from Brazil to Turkey. Single nucleotide polymorphisms were carried out using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. A total of 108 bulls, including Angus, Angus×Hereford×Nellore, Brahman, Hereford, Limousine, and Charolais breeds, were genotyped. Concerning the CAPN1, CC genotype was not found in this study. Besides, the G allele frequency was quite high (0.75). Regarding the MYF5, frequency of AA genotype was rather low (4.63%) compared to the other two genotypes, AG and GG. Therefore, the frequency of A allele was quite low (0.21). On the basis of breed-specific evaluation, the highest frequency of GG genotype of the CAPN1 was found in Brahman breed (88.24%) whereas the highest frequency of heterozygous genotype was determined in Charolais breed (85.71%). The frequency of MYF5 GG genotype was found to be very high in Limousine breed (75.00%). Moreover, MYF5 AA genotype was absent in Angus, Brahman, Limousine, and Charolais breeds. The present study may be useful for further genetic analyses conducted on beef cattle imported into Turkey.

Anahtar Kelimeler

Beef cattle, CAPN1, MYF5

Kaynakça

• 1. Allais S, Journaux L, Leveziel H, Payet-Duprat N, Raynaud P, Hocquette JF, Lepetit J, Rousset S, Denoyelle C, Bernard-Capel C, Renand G (2011): Effects of polymorphisms in the calpastatin and mu-calpain genes on meat tenderness in 3 French beef breeds. Journal of Animal Science, 89: 1-11.
• 2. Ardicli S, Samli H, Dincel D, Soyudal B, Balci F (2017): Individual and combined effects of CAPN1, CAST, LEP and GHR gene polymorphisms on carcass characteristics and meat quality in Holstein bulls. Archives Animal Breeding, 60: 303-313.
• 3. Ardicli S, Samli H, Vatansever B, Soyudal B, Dincel D, Balci F (2019): Comprehensive assessment of candidate genes associated with fattening performance in Holstein–Friesian bulls. Archives Animal Breeding, 62: 9-32.
• 4. Bhuiyan MSA, Kim N, Cho Y, Yoon D, Kim KS, Jeon JT, Lee JH (2009): Identification of SNPs in MYOD gene family and their associations with carcass traits in cattle. Livestock Science, 126: 292-297.
• 5. Botstein D, White RL, Skolnick M, Davis RW (1980): Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32: 314- 331.
• 6. Casas E, Shackelford SD, Keele JW, Koohmaraie M, Smith TP, Stone RT (2003): Detection of quantitative trait loci for growth and carcass composition in cattle. Journal of Animal Science, 81: 2976-2983.
• 7. Casas E, White SN, Riley DG, Smith TP, Brenneman RA, Olson TA, Johnson DD, Coleman SW, Bennett GL, Chase CC (2005): Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle. Journal of Animal Science, 83: 13-19.
• 8. Curi RA, Chardulo LAL, Giusti J, Silveira AC, Martins CL, de Oliveira HN (2010): Assessment of GH1, CAPN1 and CAST polymorphisms as markers of carcass and meat traits in Bos indicus and Bos taurus–Bos indicus cross beef cattle. Meat Science, 86: 915-920.
• 9. Curi RA, Krauskopf MM, Hadlich JC, Fortes MRS, Vankan DM, Augusto J, Silva V, de Oliveira HN, da Mota MDS (2012): Candidate SNPs for carcass and meat traits in Nelore animals and in their crosses with Bos taurus. Pesquisa Agropecuária Brasileira, 47: 294-301.
• 10. Falconer DS (1960): Introduction to quantitative genetics. Oliver and Boyd Ltd, Edinburgh, Great Britain, p: 58.
• 11. Gao Y, Zhang R, Hu X, Li N (2007): Application of genomic technologies to the improvement of meat quality of farm animals. Meat Science, 77: 36-45.
• 12. Gill JL, Bishop SC, McCorquodale C, Williams JL, Wiener P (2009): Association of selected SNP with carcass and taste panel assessed meat quality traits in a commercial population of Aberdeen Angussired beef cattle. Genetics, Selection, Evolution, 41: 36-47.
• 13. Kisacova J, Kubek A, Melus V, Canakyova Z, Rehout V (2009): Genetic polymorphism of Myf-5 and myostatin in Charolais breed. Journal of Agrobiology, 26: 7-11.
• 14. Lacorte G, Machado M, Martinez M, Campos A, Maciel R, Verneque RS, Teodoro RL, Peixoto MGCD, Carvalho MRS, Fonseca CG (2006): DGAT1 K232A polymorphism in Brazilian cattle breeds. Genetics and Molecular Research, 5: 475-482.
• 15. Le Hir H, Nott A, Moore MJ (2003): How introns influence and enhance eukaryotic gene expression. Trends in Biochemical Sciences, 28: 215-220.
• 16. Li C, Basarab J, Snelling W, Benkel B, Murdoch B, Hansen C, Moore SS (2004): Assessment of positional candidate genes myf 5 and igf 1 for growth on bovine chromosome 5 in commercial lines of Bos taurus. Journal of Animal Science, 82: 1-7.
• 17. Li X, Ekerljung M, Lundstrom K, Lunden A (2013): Association of polymorphisms at DGAT1, leptin, SCD1, CAPN1 and CAST genes with color, marbling and water holding capacity in meat from beef cattle populations in Sweden. Meat Science, 94: 153-158.
• 18. Lisa C, Di Stasio L (2009): Variability of μ-calpain and calpastatin genes in cattle. Italian Journal of Animal Science, 8: 99-101. 19. Miquel MC, Villarreal E, Mezzadra C, Melucci L, Soria L, Corva P, Schor A (2009): The association of CAPN1 316 marker genotypes with growth and meat quality traits of steers finished on pasture. Genetics and Molecular Biology, 32: 491-496.
• 20. Nei M, Roychoudhury A (1974): Sampling variances of heterozygosity and genetic distance. Genetics, 76: 379-390.
• 21. Page BT, Casas E, Heaton MP, Cullen NG, Hyndman DL, Morris CA, Crawford AM, Wheeler TL, Koohmaraie M, Keele JW, Smith TPL (2002): Evaluation of single-nucleotide polymorphisms in CAPN1 for association with meat tenderness in cattle. Journal of Animal Science, 80: 3077-3085.
• 22. Pintos D, Corva PM (2011): Association between molecular markers for beef tenderness and growth traits in Argentinian angus cattle. Animal Genetics, 42: 329-332.
• 23. Sambrook J, Russell DW (2006): Isolation of high-molecularweight DNA from mammalian cells using formamide, chapter 6.13. In Cold Spring Harbor protocols, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. doi:10.1101/pdb. prot3225.
• 24. Seong J, Oh JD, Cheong IC, Lee KW, Lee HK, Suh DS, Jeon GJ, Park KD, Kong HS (2011): Association between polymorphisms of Myf5 and POU1F1 genes with growth and carcass traits in Hanwoo (Korean cattle). Genes & Genomics, 33: 425-430.
• 25. Smith T, Thomas M, Bidner T, Paschal J, Franke D (2009): Single nucleotide polymorphisms in Brahman steers and their association with carcass and tenderness traits. Genetics and Molecular Research, 8: 39-46.
• 26. Trakovicka A, Moravcikova N, Kasarda R (2013): Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Acta Biochimica Polonica, 60: 783–787.
• 27. Yeh FC, Yang RC, Boyle TB, Ye Z, Mao JX (2000): POPGENE, the user-friendly shareware for population genetic analysis. Molecular biology and biotechnology centre, University of Alberta, Canada.
• 28. Zhang RF, Chen H, Lei CZ, Zhang CL, Lan XY, Zhang YD, Zhang HJ, Bao B, Niu H, Wang XZ (2007): Association between polymorphisms of MSTN and MYF5 genes and growth traits in three Chinese cattle breeds. Asian-Australasian Journal of Animal Sciences, 20: 1798-1804.