Aspartame-Induced Mitochondrial DNA Damage in Drosophila

Yıl 2018, Cilt: 9 Sayı: 2, 129 - 135, 25.04.2018

Ayşe Gül Mutlu , Nesibe Kürklü

https://doi.org/10.29048/makufebed.377982

Öz

Artificial sweeteners
are added to many products sold in the market today, such as food, beverages,
medicines, hygiene products. The possible toxic effects of any of these
products, because they are used by many people in the community, can pose a
risk for public health. DNA mutations generated by some artificial sweeteners
have been investigated by some researchers but there is no information in the
literature about the effects of these substances on mtDNA. mtDNA
damage potentially is more important than deletions in nDNA because the whole
mitochondrial genome is expressed without intron regions. Also, somatic mtDNA
mutations have been increasingly observed in primary human cancers. The aim of the current study, detection of the effects of some
artificial sweeteners on mtDNA damage and copy number in Drosophila melanogaster. The Quantitative PCR method was used for this purpose.
According to our results, Aspartame created statistically significant mtDNA damage. There was no
mtDNA damage in Saccharine+Cyclamate, Saccharine, Aceculfam K and Sucralose
application groups. These results indicate that the effects of aspartame in
human should carefully detect.

Anahtar Kelimeler

mtDNA damage, Aspartame, Saccharine, Acesulfam K, Sucralose, Cyclamate

Drosophila’da Aspartam Tarafından Oluşturulan Mitokondrial DNA Hasarı

Öz

Yapay tatlandırıcılar çok çeşitli yiyecek, içecek,
ilaç ve hijyen ürünlerine eklenir. Bu ürünlerin herhangi birinin toksik
etkilerinin olması, toplumdaki birçok insan tarafından kullanıldığı için, halk
sağlığı açısından risk oluşturabilir. Bazı yapay tatlandırıcıların nüklear DNA
üzerindeki etkileri araştırılmış, ancak bu maddelerin mtDNA üzerindeki etkileri
hakkında herhangi bir çalışmaya literatürde rastlanmamıştır. Mitokondrial
genomdaki tüm bölgeler ifade edildiğinden, mtDNA’daki mutasyonlar, nDNA’daki
delesyonlardan potansiyel olarak daha önemli olabilir. Ayrıca, primer insan
kanserlerinde somatik mtDNA mutasyonları gittikçe artan bir şekilde
gözlenmektedir. Bu çalışmanın amacı, bazı yapay tatlandırıcıların mtDNA hasarı
ve kopya sayısına etkilerinin Drosophila
melanogaster’de saptanmasıdır. Bu amaçla kantitatif PCR yöntemi
kullanılmıştır. Sonuçlarımıza göre, Aspartam istatistiksel olarak anlamlı
derecede mtDNA hasarı oluşturmuştur. Sakkarin + Siklamat, Sakkarin, Asesulfam K
ve Sukraloz uygulama gruplarında ise mtDNA hasarı saptanmamıştır. Bu sonuçlar,
Aspartamın insanlar üzerinde yapabileceği olası etkilerinin dikkatle saptanması
gerektiğini göstermektedir.

Anahtar Kelimeler

mtDNA hasarı, Aspartam, Sakkarin, Asesulfam K, Sukraloz, Siklamat

Kaynakça

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