Assessment of the Impact of Curcumin on Cell Cultures Derived from the Primary Intervertebral Disc Tissue in Humans

Yıl 2023, Cilt: 15 Sayı: 2, 49 - 55, 31.08.2023

Mehmet Albayrak , İbrahim Yılmaz , Muharrem Furkan Yüzbaşı , Hande Akalan , Duygu Yaşar Şirin , Numan Karaarslan , Hanefi Özbek , Ozkan Ates

Öz

Aim: Degenerative disc disease in the lumbar spine is widely observed. Degenerative disc diseases are among the causes of low back pain in older age. Modern drug discovery studies have aimed to identify potential molecules that target multiple pathways with a safer profile against degeneration. This study aimed to evaluate the effects of curcumin, a natural phenolic compound, on primary cell cultures prepared using intervertebral disc (IVD) tissues resected during the surgeries of patients with lumbar disc herniation.
Materials and Methods: Primary cell cultures were prepared using human IVD tissues of eight patients. Untreated groups served as the control and curcumin-treated groups as the study sample. In-vitro cytotoxicity analyses were performed in all groups. Acridine orange (AO)/propidium iodide (PI) and Janus Green B staining were performed to evaluate cell surface morphologies. One-way analysis of variance and Tukey HSD, a multiple comparison test, were used to assess the obtained data.
Results: Proliferation slightly increased as of 24 h in the curcumin-treated samples, but decreased in the 48 and 72 hour curcumin-treated samples compared to the control samples. The obtained results were statistically significant (p<0.05). Additionally no cytotoxicity was observed according to morphological evaluations.
Conclusion: This research is an in-vitro experimental study. However, this natural and non-toxic pleiotropic agent can be targeted to cell-damaged sites through appropriate drug delivery systems. It may thus be a safe treatment option for the regeneration of degenerated lumbar IVD cells in the future.

Anahtar Kelimeler

Annulus fibrosus, Curcumin, Cytotoxicity, Nucleus pulposus, Primary cell culture, Proliferation.

İnsan Primer İntervertebral Disk Dokusundan Elde Edilen Hücre Kültürleri Üzerinde Curcumin'in Etkisinin Değerlendirilmesi

Öz

Amaç
Lomber omurga bölgesinde dejeneratif disk hastalığı yaygın olarak gözlemlenmektedir. Dejeneratif disk hastalıkları, yaşlılıkta bel ağrısının nedenlerinden biridir. Modern ilaç keşfi çalışmaları, daha güvenli bir profille dejenerasyona karşı çoklu yolları hedefleyebilen potansiyel molekülleri tanımlamayı amaçlamıştır. Bu çalışmanın amacı, doğal bir fenolik bileşik olan kurkuminin, lomber disk herniasyonu olan hastaların cerrahileri sırasında çıkarılan intervertebral disk (IVD) dokuları kullanılarak hazırlanan primer hücre kültürleri üzerindeki etkilerini değerlendirmektir.
Materyal ve Metotlar
Sekiz hastanın insan IVD dokuları kullanılarak primer hücre kültürleri hazırlandı. Tedavi edilmemiş gruplar kontrol grubu olarak hizmet etti, kurkumin tedavisi uygulanan gruplar ise çalışma örneği olarak kullanıldı. Tüm gruplarda in-vitro sitotoksisite analizleri yapıldı. Hücre yüzey morfolojilerini değerlendirmek için Akrin Orange (AO)/Propidyum İodür (PI) ve Janus Green B boyaması yapıldı. Elde edilen verileri değerlendirmek için tek yönlü varyans analizi ve çoklu karşılaştırma testi olarak Tukey HSD kullanıldı.
Bulgular
Kurkumin tedavisi uygulanan örneklerde 24 saat sonra hafif bir şekilde çoğalma görülürken, 48 ve 72 saatlik kurkumin tedavisi uygulanan örneklerde kontrol örneklerine kıyasla azaldı. Elde edilen sonuçlar istatistiksel olarak anlamlıydı (p<0.05). Ayrıca morfolojik değerlendirmelere göre sitotoksisite gözlenmedi.
Sonuç
Bu araştırma in-vitro deneysel bir çalışmadır. Ancak, bu doğal ve toksik olmayan çok yönlü ajan uygun ilaç taşıma sistemleri aracılığıyla hücre hasarlı bölgelere yönlendirilebilir. Bu nedenle gelecekte dejeneratif lomber IVD hücrelerinin rejenerasyonu için güvenli bir tedavi seçeneği olabilir.

Anahtar Kelimeler

Anulus fibrozus, Çoğalma, Kurkumin, Nükleus pulpozus, Primer hücre kültürü, Sitotoksisite

Kaynakça

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