The ameliorating effects of apigenin and chrysin alone and in combination on polycystic ovary syndrome induced by dehydroepiandrosterone in rats

Yıl 2024, , 198 - 207, 31.05.2024

Buket Berk , Nevin İlhan , Solmaz Susam , Fatma Tedik , Nalan Kaya Tektemur

https://doi.org/10.5472/marumj.1479311

Öz

Objective: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder among women of reproductive age and is
one of the main causes of ovulation infertility, affecting 5-10% of women. Inflammation, hormonal imbalances, and disruption of the
oxidant-antioxidant balance are the main factors in the pathophysiology of PCOS. This study was designed to answer the question of
whether apigenin and chrysin have therapeutic effects on the dehydroepiandrosterone (DHEA)-induced rat model of PCOS.
Materials and Methods: The experimental PCOS model was created by administering 6 mg/100g DHEA subcutaneously to 21-day-old
female Wistar rats for 28 days, followed by treatment with natural agents 50 mg/kg apigenin and 50 mg/kg chrysin by oral gavage twice
a week for one month. The predominant cell type was determined by microscopic analysis in vaginal smears daily from day 10 to day
28 of the experiment. In tissue supernatants, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, and
malondialdehyde (MDA) levels were obtained by spectrophotometric method with appropriate manual methods; follicle-stimulating
hormone (FSH), luteinizing hormone (LH), progesterone, interleukin (IL)-18, IL-1β, and IL-13 levels were determined by enzymelinked
immunosorbent assay (ELISA) method. In addition, histological sections obtained from ovarian tissue samples were stained
with hematoxylin-eosin and examined under a light microscope.
Results: The results showed that treatment with apigenin and chrysin alone and in combination reduced MDA, LH, FSH, progesterone,
IL-1β, IL-13, and IL-18 levels compared with PCOS rats. Furthermore, enzymatic activities of antioxidants including CAT, SOD, and
GPx in the ovaries increased in therapeutic groups compared to the PCOS group.
Conclusion: In conclusion, this study demonstrates the potential therapeutic efficacy of apigenin and chrysin, either alone or in
combination, in alleviating the hormonal imbalances, inflammation, and oxidative stress in DHEA-induced PCOS rats. Apigenin,
in particular, emerges as a promising agent for PCOS treatment, showing superiority over chrysin and combination treatments in
ameliorating cystic follicles and improving various parameters associated with PCOS pathophysiology. These findings suggest that
apigenin holds promise as a novel therapeutic agent for PCOS and warrants further investigation in clinical settings.

Anahtar Kelimeler

Polycystic ovary syndrome, Dehydroepiandrosterone, Apigenin, Chrysin

Kaynakça

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