Sıçanlarda yanık ile uyarılan uzak organ hasarı ve minosiklinin etkisi

Yıl 2017, Cilt: 30 Sayı: 3, 137 - 145, 29.10.2017

Elif Bahadır Murat Sancar Gazi Giray Tarhan Emre Koçak Zeynep Budancamanak Seda Karaaslan Elif Kervancıoğlu Demirci Meral Yüksel Feriha Ercan İnci Alican

https://doi.org/10.5472/marumj.370641

Cited By: 3

Öz

Amaç: Bu çalışma, oral uygulanan minosiklinin -yarı sentetik

ikinci kuşak tetrasiklin– sıçanlarda yanık ile uyarılan karaciğer ve

böbrek hasarı üzerine anti-inflamatuvar potansiyelini incelemeyi

amaçlamıştır.

Gereç ve Yöntem: Çalışmada dişi Sprague-Dawley sıçanlar

(250-300 g; n=7-8/grup) kullanıldı. Yanık ve taklit grupları, sırası

ile 90 0C ve 25 0C’lik su banyosunda 10 saniye tutuldu. Minosiklin

(20 mg/kg; günde iki kez; orogastrik yolla) yanık sonrası 24

saat süreyle uygulandı. Dekapitasyon sonrası, biyokimyasal

ölçümler için gövde kanı toplandı. Histopatolojik inceleme

ve malondialdehit (MDA), glutatyon ve kemiluminisans (CL)

ölçümleri için karaciğer ve böbrekler çıkarıldı.

Bulgular: Minosiklin tedavisi yanık grubuna ait serum alanin

aminotransferaz, aspartat aminotransferaz, kan üre azotu ve

kreatinin düzeylerine anlamlı bir etki göstermedi. Yanık grubunda

artmış serum toplam oksidan durum (P<0.001) minosiklin ile geri

döndürüldü (P<0.001) ve karaciğer mikroskopik hasar skoru hafif

azalma (P<0.01) gösterdi. Minosiklin yanık grubunda artmış doku

MDA ve glutatyon düzeyleri üzerine anlamlı etki göstermedi ancak

artmış luminol CL düzeylerini geri döndürdü (P<0.001, karaciğer

ve P<0.01, böbrek).

Sonuç: Sıçanlara yanık sonrası 24 saat minosiklin tedavisi

karaciğer ve böbrekte oksidan oluşumunu azaltmada etkin

bulunmuştur; ancak bu dokuları oksidan hasara karşı hafif derecede

koruyucu bir etki göstermiştir.

Anahtar Kelimeler

Böbrek, Karaciğer, Minosiklin, Sıçan, Yanık

Burn-induced distant organ injury in rats and the effect of minocycline

Öz

Objectives: This study aimed to examine the anti-inflammatory

potential of orally-administered minocycline, a semi-synthetic

second-generation tetracycline, on burn-induced liver and kidney

damage in rats.

Materials and Methods: Female Sprague-Dawley rats (250-

300 g; n=7-8/group) were used. Burn and sham groups were

exposed to 90 0C and 25 0C water bath for 10 s, respectively.

Minocycline (20 mg/kg; twice daily; orogastrically) was

administered for 24 h post-burn. After decapitation, trunk blood

was collected for biochemical assays. Liver and kidneys were

excised for histopathological evaluation and malondialdehyde

(MDA), glutathione and chemiluminescence (CL) assays.

Results: Minocycline treatment did not exert a significant effect

on serum alanine aminotransferase, aspartate aminotransferase,

blood urea nitrogen, and creatinine levels of the burn group.

Increased serum total oxidant status of the burn group was reversed

(P<0.001) and liver microscopic lesion score showed a slight

reduction (P<0.01) by minocycline. Minocycline did not cause a

significant effect on increased tissue MDA and glutathione levels

of the burn group but reversed the elevated luminol CL levels

(P<0.001, for liver and P<0.01, for kidney, respectively).

Conclusion: Minocycline treatment to rats for 24 h post-burn

was found to be effective to reduce oxidant production in the liver

and kidney; however, it showed slight protection on these tissues

against oxidant damage.

Anahtar Kelimeler

Burn, Kidney, Liver, Minocycline, Rat

Kaynakça

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