Metabolite Production and Antibacterial Activities of Callus Cultures from Rosa damascena Mill. Petals

Yıl 2017, Cilt: 21 Sayı: 3, 590 - 597, 20.06.2017

Pınar Olgunsoy Seyhan Ulusoy , Ufuk Çelikkol Akçay

https://doi.org/10.12991/marupj.319331

Öz

Rosa damascena Mill. is a very fragrant member of Rosaceae

family, exhibiting strong antioxidant and antimicrobial activity.

In this study, in vitro cell cultures from Rosa damascena Mill.

flower petals were established and the effects of precursor

L-phenylalanine, the elicitor methyl jasmonate and light/dark

treatments on the accumulation of characteristic rose volatile

compounds, tocopherols and β-carotene in addition to the

antibacterial activity of petal callus extracts were investigated

with Solid-Phase Microextraction Combined with Gas

Chromatography–Mass Spectrometry (GC–MS). The amount

of β-pinene increased by 236 times when 500 μM Phe was

added to the culture medium or under light treatment. Strong

antibacterial activity from petal callus extracts, up to 2.6-

fold compared to amikacin as well as original petal tissues,

was obtained under dark treatment for P. aeruginosa ATCC

27853 and S. aureus ATCC 25923. These results revealed that

petal callus extracts are sources of natural antioxidants and

antibacterial compounds.

Anahtar Kelimeler

Rosa damascena Mill., flower petals, callus, secondary metabolite, antibacterial activity

Kaynakça

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Bu çalışmada Rosa damascena Mill. çiçek petallerinden in vitro hücre kültürü yapılmış, bu kültürlerden hazırlanan ekstrelerde L-fenilalanin, metil jasmonat, ışık/ karanlık uygulamasının gül uçucu bileşenleri, tokoferol, β-karoten üretimine etkisi ve antibakteriyel özellikleri incelenmiştir. Petal hücre ekstrelerinde, terpeneoidler (alfa/beta pinen) fenileten, kaproaldehit and benzaldehit bulunduğu SPME/GC-MS ile tespit edilmiştir. Işık veya 500 μM fenilalanin uygulaması ile beta pinen miktarının 236 kat arttığı belirlenmiştir. Karanlık uygulaması ile kültür ekstrelerinin antibakteriyel etkilerinin P. aeruginosa ATCC 27853 ve S. aureus ATCC 25923 için kanamisine göre 2.6 kat arttığı belirlenmiştir. Bu sonuçlar, petal kallus ekstrelerinin doğal antioksidan ve antibakteriyel moleküller içerdiğini göstermektedir. Anahtar kelimeler: Rosa damascena Mill., çiçek petalleri, kallus, sekonder metabolit, antibakteriyel aktivite 5. Da Porto C, Decorti D, Natolino A. 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