Microorganisms that Reproduce in Wound Cultures in Rize Region and Their Antimicrobial Susceptibility

Yıl 2020, Cilt: 6 Sayı: 3, 358 - 368, 31.12.2020

İlkay Bahçeci

https://doi.org/10.19127/mbsjohs.823556

Cited By: 3

Öz

Objective: Among nosocomial infections, wound infections are one of the important factors causing mortality and morbidity after urinary tract infections. In this study, it was aimed to guide the empirical treatment and to contribute to epidemiological data by determining the microorganism and antimicrobial susceptibility which have been isolated from cultures of wounds and abscesses, which came from outpatient clinics, services and intensive care units, that grow in wound site and abscess cultures that comes from outpatient clinics, services and intensive care units.
Methods: This study was conducted on the basis of cultures in which at least one microorganism grew in the wound site and abscess samples from the polyclinic, service and intensive care units during routine application at the Recep Tayyip Erdoğan University Training and Research Hospital Microbiology Laboratory, between January 2011 and December 2016. Samples were taken with two sterile swabs in the form of superficial swabs or deep aspiration and delivered to the laboratory with transport medium as soon as possible. Gram staining preparation was prepared and inoculated on 5% sheep blood agar, eosin-methylene-blue agar, chocolate agar and sabouraud dextrose agar. The gram-stained preparation was evaluated by Q scoring. Evaluation of the culture and antibiogram susceptibility were made according to the Clinical and Laboratory Standards Institute (CLSI) criteria.
Results: A total of 2202 samples were received, and reproduction was observed in 930 samples. Among the 793 wound and 137 abscess samples, the most common microorganism was Staphylococcus aureus (224 cultures) and the second was Escherichia coli (135 cultures).
Conclusion: Surgery clinics, especially orthopedics, provided the most common wound infections, from which S. aureus was the most isolated microorganism. The fact that bacterial, most notably S. aureus, propagation occurred from surgery samples indicates that surgical site infections are generally caused by endogenous flora. It has, thus, become apparent in our study that patients and hospital staff should pay more attention to hygiene, especially hand washing. Due to the changing of the distribution and resistance patterns of microorganisms that are frequently seen in hospitals at certain time intervals, their antibiotic susceptibility will be a guide in the rational use of antibiotics. Thus, specific treatment will contribute to the saving on the cost and reduce mortality.

Anahtar Kelimeler

abscess, antimicrobial susceptibility, microorganısm, wound culture

Destekleyen Kurum

-

Proje Numarası

-

Teşekkür

-

Kaynakça

• Akinjogunla OJ, Adegoke AA, Mboto CI, Chukwudebelu IC, Udokang IP. Bacteriology of automobile accident wounds infection. Int J MedSci 2009;1: 23-7.
• Altan G, Mumcuoğlu I, Hazirolan G, Dulger D, Aksu N. Microorganisms which isolated from wound samples and their antimicrobial susceptibilities. Turkish Bulletin of Hygiene and Experimental Bıology. 2017; 74 (4): 279-86.
• Atiyeh BS, Ioannovich J, Al-Amm CA, El-Musa KA. Management of acute and chronic open wounds: the importance of moist environment in optimal wound healing. Curr Pharm Biotechnol 2002; 3: 179.
• Avcioglu F, Behcet M, Karabork S, Kurtoglu M. Antimicrobial Resistance Rates of Microorganisms Isolated from Wound Specimens – Three Year Evaluation Journal of Duzce University Health Sciences Institute / J DU Health Sci. 2019; 9(3): 110- 4.
• Bessa LJ, Fazii P, DiGiulio M, Cellini L. Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection. Int Wound J. 2015;12 (1): 47-52.https://doi.org/10. 1111/iwj.12049
• Bhatt C, Lakhey M. The distribution of pathogens causing wound infection and their antibiotic susceptibility pattern. J Nepal Health Res Council 2006;5(1):22–6 Broughton G 2nd, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconstr Surg 2006; 117:12S. Cirit OS, Muderris T, Mizrakli AZ, Vurupalmaz Y, Baris A. Aerobic Bacteria Isolated from the Wound Cultures and Their Antibiotic SusceptibilitiesTurkish Microbiyological Society, 2014; 44(1): 149-57.
• Citil MU, Mete E, Oguz EO, Abban GM, Sahin B, Kaleli I. Investigation of the effect of ibuprofen on wound healing in experimental Staphylococcus aureus soft tissue infections. Microbiyology Bulletin. 2015; 49(2): 166-80
• Copeland-Halperin LR, Kaminsky AJ, Bluefeld N, Miraliakbari R. Sample procurement for cultures of infected wounds: a systematic review. J Wound Care 2016;25(4):4-6, 8-10. https://doi.org/10.12968/jowc.2016. 25.Sup4.S4
• Davarci I, Kocoglu ME, Barlas N, Samasti M. Antimicrobial Susceptibilities of Bacteria Isolated from Wound Cultures: A Three-Year Evaluation. ANKEM. 2018;32(2):53-61.
• Dogan SS, Pakoz NIE, Aral M. The Distribution and Antibiotic Susceptibility of the Microorganisms Isolated from Wound Specimens Turkish Microbiyological Society, 2010;40 (4):243-9
• Espejo E, Andres M, Borrallo R-M, Padilla E, Garcia Restoy E, Bella F. Bacteremia Associated with Pressure Ulcers: A Prospective Cohort Study. Eur J Clin Microbiol Infect Dis. 2018;37(5):969-75.
• Gundem NS, Cikman A. Microorganisms Isolated from Wound Cultures, and their Antibiotic Susceptibilities ANKEM, 2012;26 (4):165-70
• Mulu W, Kibru G, Beyene G, Damtie M. Postoperative nosocomial infections, and antimicrobial resistance pattern of bacteria isolates among patients admitted at Felege Hiwot Referral Hospital, Bahirdar, Ethiopia. Ethiop J Health Sci 2012;22(1):7-1
• Ozturk I, Oztin H. The Distribution of Agents and Antibiotic Resistance in Decubitus Ulcer Infection. Medical Journal of Mugla Sitki Kocaman University 2020;7(1):1-5
• Owens CD, Stoessel K. Surgical site infections: Epidemiology, microbiology, and prevention. Journal of Hospital Infection. 2008;70 (Suppl 2): 3-10.
• Schnuriger B, Kenji Inaba K, Eberle BM, Wu T, Talving P, Bukur M, et al. Microbiological profile, and antimicrobial susceptibility in surgical site infections following hollow viscus injury. J Gastrointest Surg. 2010;14:1304–10. https://doi.org/10.1007/s11605-010-1231-x Schultz GS, Sibbald RG, Falanga V, Ayello EA, Dowsett C, Harding K. et al. Wound bed preparation: a systematic approach to wound management. Wound Repair Regen 2003; 11 (1):1-28. doi: 10.1046/j.1524-475x.11.s2.1.x.
• Sesli E, Kaya S, Tas T, Aridogan CB, Demirci M. Microorganism profile and antibioticsusceptibility status of surgical site infections. ANKEM 2006; 20(2): 89-93.
• TMC-ADTS Restricted Notice Table. Turkish Microbiology Society.http://www.tmc-online.org/userfiles/file/26-37.pdf (2017)
• Turhanoglu MN, Koyuncu E, Bayindir-Bilman F. Microorganisms and antibiotic resistances isolated from wound cultures, 2010-2015 Turkish Bulletin of Hygiene and Experimental Biology, 2018; 75(2): 183-194
• Yurtsever SG, Kurultay N, Ceken N, Yurtsever S, Afsar I, Sener AG, et.al. Evaluation of the Microorganisms Isolated from Wound Specimens and their Antibiotic Susceptibility. ANKEM 2009; 23(1): 34-8.
• Wayne PA Clinical andLaboratory Standards Institute; CLSI. Performance Standards for Antimicrobial Susceptibility Testing: Document2011-2016. CLSI
• Wong SY, Manikam R, Muniandy S. Prevalence, and antibiotic susceptibility of bacteria from acute and chronic wounds in Malaysian subjects. J Infect Dev Ctries 2015;9(9):936-44. https://doi.org/10. 3855/jidc.5882