Ramelteon used to treat insomnia can reduce the occurrence of osteoporosis

Yıl 2021, Cilt: 5 Sayı: 2, 164 - 170, 30.08.2021

Duygu Köse , Ahmet Köse , Zekai Halıcı , Muhammed Ali Gürbüz Adem Maman , Muhammed Yayla

https://doi.org/10.30565/medalanya.939161

Cited By: 1

Öz

Aim: Melatonin promoted osteoblast differentiation and causes an increase in levels of markers of bone differentiation and proliferation. Ramelteon (RAMEL) activates melatonin receptors and binds to these receptors as non-selective. In this study, we investigated the preventive effects of the melatonin agonist RAMEL on osteoporosis by radiological, histological, and molecularly.

Methods: Groups 1: Control, Group 2: Osteoporosis: Overectomized group (OP), Group 3: OP + ramelteon 2 mg/kg, Group 4: OP + ramelteon 4 mg/kg. 24 animals underwent bilateral ovariectomy. RAMEL was administered orally once a day in the prophylactic treatment mode for 8 weeks, 6 weeks after ovariectomy.

Results: Fourteen weeks after ovariectomy, there was a significant reduction in femoral bone mineral density (BMD) (g/cm2) in the OP group compared to the control group. Compared to the OP group, RAMEL treatment significantly increased the BMD level (p<0.05). Bone matrix protein 2 (BMP2) and runt-related transcription factor 2 (RUNX2) mRNA levels were significantly lower in the OP group than in the control group (p<0.05). RUNX2 and BMP2 mRNA levels were significantly higher in the RAMEL treatment groups than in the OP group (p<0.05).

Conclusion: To take advantage of the peripheral effects of melatonin, RAMEL, a peripheral melatonin agonist, can be used to prevent osteoporosis. 

Anahtar Kelimeler

melatonin;, bone;, rameltone;, osteoporosis;

Uykusuzluğu tedavi etmek için kullanılan Ramelteon, osteoporoz oluşumunu azaltabilir

Öz

Amaç: Melatonin osteoblast farklılaşmasını teşvik eder ve kemik farklılaşması ve proliferasyon belirteçlerinin düzeylerinde artışa neden olur. Ramelteon (RAMEL) melatonin reseptörlerini aktive eder ve bu reseptörlere seçici olmayan şekilde bağlanır. Bu çalışmada melatonin agonisti RAMEL' in osteoporoz üzerindeki önleyici etkileri radyolojik, histolojik ve moleküler olarak araştırıldı.

Yöntem: Grup 1: Kontrol, Grup 2: Osteoporoz: Over eksizyonu yapılan grup (OP), Grup 3: OP + ramelteon 2 mg/kg, Grup 4: OP + ramelteon 4 mg/kg. 24 hayvana, bilateral ovariektomi uygulandı. RAMEL, ovariektomiden 6 hafta sonra, 8 hafta boyunca profilaktik tedavi modunda günde bir kez oral yoldan uygulandı.

Bulgular: Ovariektomiden on dört hafta sonra, kontrol grubuna kıyasla OP grubunda femoral kemik mineral yoğunluğunda (KMY) (g/cm2) anlamlı bir azalma oldu. OP grubu ile karşılaştırıldığında, RAMEL tedavisi BMD seviyesini önemli ölçüde artırdı (p<0.05). Bone matrix protein 2 (BMP2) and runt-related transcription factor 2 (RUNX2) mRNA seviyeleri OP grubunda kontrol grubuna göre anlamlı derecede düşüktü (p<0.05). RUNX2 ve BMP2 mRNA seviyeleri, RAMEL tedavi gruplarında OP grubuna göre anlamlı olarak daha yüksekti (p<0.05).

Sonuç: Melatoninin periferik etkilerinden yararlanmak için, bir periferik melatonin agonisti olan RAMEL, osteoporozu önlemek için kullanılabilir.

Anahtar Kelimeler

Ramelteon, melatonin, osteoporoz;, kemik;

Kaynakça

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