Vitamin D, VDR, and VDBP Levels Correlate with Anti-inflammatory Cytokine Profile in FMS Patients

Yıl 2023, Cilt: 5 Sayı: 1, 24 - 8, 15.01.2023

Pınar Ellergezen , Alev Alp , Sinan Çavun

https://doi.org/10.37990/medr.1131305

Cited By: 1

Öz

Aim: The major target of this research is to examine whether there is any connection between the levels of vitamin D and anti-inflammatory mediators in patients with fibromyalgia syndrome (FMS).
Materials and Methods: The study contains 30 FMS diagnosed and 25 healthy female individuals and the determination of FMS was made according to the standards of 2010 American College of Rheumatology (ACR). Vitamin D, vitamin D receptor (VDR), vitamin D binding protein (VDBP) levels, and anti-inflammatory cytokine (IL-4, IL-10, TGF-β) levels in the serum of patients with FMS and healthy individuals were measured using enzyme-linked immunosorbent assay (ELISA).
Results: The concentrations of vitamin D, VDR, and VDBP were determined to be higher in healthy controls than in patients with FMS (p<0.001). Correlating with this, IL-4, IL-10, and TGF-β levels were measured remarkably higher in the healthy group than in the FMS patients (p<0.001).
Conclusion: Low vitamin D levels may cause a decrease in anti-inflammatory cytokine levels and their immunosuppressive effect in FMS.

Anahtar Kelimeler

anti-inflammatory cytokines, vitamin D, fibromyalgia syndrome

Destekleyen Kurum

Uludağ Üniversitesi Bilimsel Araştırma ve Proje Birimi

Proje Numarası

KUAP (T) 2020/2

Teşekkür

We would like to thank all the volunteers who participated in this study and the medical pharmacology department for supporting the laboratory studies.

Kaynakça

• 1. Clauw DJ, Arnold LM, McCarberg BH. The science of fibromyalgia. Mayo Clin Proc. 2011;86:907–11.
• 2. Clauw DJ. Fibromyalgia: a clinical review. JAMA. 2014;311:1547–55.
• 3. Zhang JM, An J. Cytokines, Inflammation and Pain. Int Anesthesiol Clin. 2007;45:27–37.
• 4. Uceyler N, Valenza R, Stock M, et al. Reduced levels of antiinflammatory cytokines in patients with chronic widespread pain. Arthritis Rheum. 2006;54:2656–64.
• 5. Unsicker K, Flanders KC, Cissel DS, et al. Transforming growth factor beta isoforms in the adult rat central and peripheral nervous system. Neuroscience. 1991;44:613-25.
• 6. Roberts AB, Sporn MB. Physiological actions and clinical applications of transforming growth factor-beta (TGF-beta). Growth Factors. 1993;8:1–9.
• 7. Ding A, Nathan CF, Graycar J, et al. Macrophage deactivating factor and transforming growth factors-beta 1 -beta 2 and -beta 3 inhibit induction of macrophage nitrogen oxide synthesis by IFN-gamma. J Immunol. 1990;145:940–4.
• 8. Wang TT, et al. Cutting edge: 1,25-dihydroxyvitamin D3 is a direct inducer of antimicrobial peptide gene expression. J Immunol. 2004;173:2909–12.
• 9. Fleet JC, Schoch RD. Molecular mechanisms for regulation of intestinal calcium absorption by vitamin d and other factors. Crit Rev Clin Lab Sci. 2010;47:181–95.
• 10. Provvedini DM, Tsoukas CD, Deftos LJ, Manolagas SC. 1,25-dihydroxyvitamin D3 receptors in human leukocytes. Science. 1983;221:1181-3.
• 11. Abdella NA, Mojiminiyi OA. Vitamin D-Binding protein clearance ratio is significantly associated with glycemic status and diabetes complications ina predominantly vitamin D-deficient population. J Diabetes Res. 2018, Article ID:6239158.
• 12. Makrani AH, Afshari M, Ghajar M et al. Vitamin D and fibromyalgia: a meta-analysis. Korean J Pain. 2017;30:250–7.
• 13. Baygutalp NK, Baygutalp F, Şeferoğlu B, Bakan E. The relation between serum vitamin D levels and clinical findings of fibromyalgia syndrome. Dicle Med J. 2014;41:446-50.
• 14. Marchand F, Peretti M, McMahon SB. Role of the Immune system in chronic pain. Nat Rev Neurosci. 2005;6:521–32.
• 15. Okyay R, Koçyigit B, Gürsoy S. Vitamin D levels in women with fibromyalgia and relationship between pain, tender point count and disease activity. Acta Med Mediterr. 2016;32:243–7.
• 16. Labeeb AA, Al-Sharaki DR. Detection of serum 25(OH)-vitamin D level in the serum of women with fibromyalgia syndrome and its relation to pain severity. Egypt Rheumatol Rehabil. 2015;42:196–200. 17. Holick MF. Vitamin D deficiency. N Engl J Med. 2007;357:266-81.
• 18. Brannon PM. Key questions in Vitamin D research. Scand J Clin Lab Invest. 2012;243:154–62.
• 19. Boonstra A, Barrat FJ, Crain C et al. 1alpha,25- Dihydroxyvitamin d3 has a direct effect on naive CD4(+) T cells to enhance the development of Th2 cells. J Immunol. 2001;167:4974.
• 20. Norman AW. From vitamin D to hormone D: fundamentals of the vitamin D endocrine system essential for good health. Am J Clin Nutr. 2008;88:491–9.
• 21. Kumar NP, Gopinath V, Sridhar R et al. IL10 dependent suppression of type 1, type 2 and type 17 cytokines in active pulmonary tuberculosis. PLoS One. 2013;8:e59572.
• 22. Song Y, Hong J, Liu D et al. 1,25-dihydroxyvitamin D3 inhibits nuclear factor kappa B activation by stabilizing inhibitor IkappaBalpha via mRNA stability and reduced phosphorylation in passively sensitized human airway smooth muscle cells. Scand J Immunol. 2013;77:109.
• 23. Khoo AL, Chai LY, Koenen HJ et al. Vitamin D(3) down-regulates proinflammatory cytokine response to Mycobacterium tuberculosis through pattern recognition receptors while inducing protective cathelicidin production. Cytokine. 2011;55:294.
• 24. Harishankar M, Afsal K, Banurekha VV et al. 1,25-Dihydroxy vitamin D3 downregulates pro-inflammatory cytokine response in pulmonary tuberculosis. Int Immunopharmacol. 2014; 23:148.
• 25. Plotnikoff GA, Quigley JM. Prevalence of severe hypovitaminosis D in patients with persistent, nonspecific musculoskeletal pain. Mayo Clin Proc. 2003;78:1463–70.
• 26. Watkins LR, Milligan ED, Maier SF. Glial proinflammatory cytokines mediate exaggerated pain states: implications for clinical pain. Adv Exp Med Biol. 2003;521:1–21.
• 27. Uceyler N, Valenza R, Stock M, et al. Reduced levels of antiinflammatory cytokines in patients with chronic widespread pain. Arthritis Rheum. 2006;54:2656–64.
• 28. Milligan ED, Sloane EM, Langer SJ, et al. Controlling neuropathic pain by adeno-associated virus driven production of the anti-inflammatory cytokine, interleukin-10. Mol Pain. 2005;1:9.
• 29. Meller ST, Gebhart GF. Nitric oxide (NO) and nociceptive processing in the spinal cord. Pain. 1993;52:127–36.
• 30. Garcion E, Wion-Barbot N, Montero-Menei CN et al. New clues about vitamin D functions in the nervous system. Trends Endocrinol Metab. 2002;13:100–5.
• 31. Mahon BD, Wittke A, Weaver V, Cantorna MT. The targets of vitamin D depend on the differentiation and activation status of CD4 positive T cells. J Cell Biochem. 2003;89:922–32.
• 32. Piantoni S, Andreoli L, Scarsi M, et al. Phenotype modifications of T-cells and their shift toward a Th2 response in patients with systemic lupus erythematosus supplemented with different monthly regimens of vitamin D. Lupus. 2015;24:490–8.