Chemical Content and Bioactive Properties of Drone Larvae (Apilarnil)

Yıl 2019, Cilt: 19 Sayı: 2, 14 - 22, 27.12.2019

Sibel Silici

Öz

Bu çalışmada, erkek arı larvasının (apilarnil) besin elementleri, aminoasit içeriği ve biyoaktif özellikleri belirlenmiştir. HPLC-UV ile yapılan analiz sonuçlarına göre apilarnil’de 16 amino asit belirlenmiştir. Esansiyel amino asitlerden yalnızca triptofan tespit edilememiştir. En yüksek amino asit miktarı 7198 mg/100g olan lizin amino asidi iken en düşük metionin amino asidi olup 500 mg/100g olarak belirlenmiştir. Elde edilen sonuçlara göre apilarnilin nem, kül, protein, karbonhidrat ve lipit içeriği sırasıyla 4.43, 4.07, 48.75, 21.62 ve 21.13 g/100 g dır. Apilarnilin biyoaktif özelliklerini belirlemede fenolik madde içeriği için Folin-Ciocalteu metodu, antioksidan aktivite için fosfomolibden metodu ve antiradikal aktivite için DPPH metodu kullanılmıştır. Apilarnilin toplam fenolik madde içeriği 834,05 (mg GAE/100 g) iken antioksidan aktivitesi 90,91 mg AAE/g bulunmuştur. Erkek arı larvasının (apilarnil) antiradikal aktivite inhibisyon düzeyi % 81.61 olarak tespit edilmiştir. Sonuç olarak, apilarnilin faydalı biyolojik aktivitesi antioksidan aktivitesinden kaynaklanıyor olabilir.

Anahtar Kelimeler

Apilarnil, drone larvae

Kaynakça

• 1 YÜCEL, B; KÖSOĞLU, M (2015). Apiterapide apilarnil. Arı Ürünleri ve Sağlık. Sidaş Yayıncılık, İzmir. ISBN: 9786055267261 2 YÜCEL, B; AÇIKGÖZ, Z; BAYRAKTAR, H; SEREMET, Ç (2011). The effects of apilarnil (drone bee larvae) administration on growth performance and secondary sex characteristics of male broilers. J Anim Vet Adv, 10 (17): 2263-2266. 3 ALTAN, Ö; YÜCEL, B; AÇIKGÖZ, Z; SEREMET, C; KÖSOĞLU, M; TURGAN, N; ÖZGÖNÜL, AM (2013). Apilarnil reduces fear and advances sexual development in male broilers but has no effect on growth. Br Poult Sci, 54(3):355-361. 4 BOLATOVNA, KS; RUSTENOV A; ELEQALİEVA, N; OMİRZAK, T; AKHANOV, UK (2015). Improving reproductive qualities of pigs using the drone brood homogenate. Biol Med (Aligarh), 7 (2): BM-091-15. 5 SHOINBAYEVA, KB; OMIRZAK, T; BIGARA T; ABUBAKIROVA A, DAUYLBAY A (2017). Biologically active preparation and reproductive function of stud rams. Asian Journal of Pharmaceutics (AJP): Free full text articles from Asian J Pharm 11(03): 184-191. 6 VALER’EVNA ZE, IVANOVİCH BG, VİKTOROVİCH NA,GENNADEVİCH KO, MİKRTİCHİAN MG, VALERİEVNA ZJ, KUZMİNİCHNA KE. (2018) Hormonal Status and Productıve Qualıtıes of Young Pıgs at Inclusıon In a Dıet Feedıng Homogenate Drone Brood, Сельскохозяйственные науки (Agric Sci) 636: 612-636. 7 BALKANSKA, R; KARADJOVA, I; IGNATOVA, M (2014). Comparative analyses of chemical composition of royal jelly and drone brood. Bulg Chem Commun 46(2): 412-416. 8 BARNUTIU, LI; MARGHİTAŞ, LA; DEZMİREAN, D; BOBIŞ, O; MIHAı, C; PAVEL, C (2013). Physico-chemical composition of Apilarnil (Bee drone larvae). Seria Zootechnie, 59:199-202. 9 FINKE, MD (2005). Nutrient composition of bee brood and its potential as human food. Ecol Food Nutr, 44(4): 257-270. 10 GOINS, A; SCHNEIDER, SS (2013). Drone “quality” and caste interactions in the honey bee, Apis mellifera L. Insectes sociaux, 60(4): 453-461. 11 HRYNIEWICKA, M; KARPINSKA, A; KIJEWSKA, M; TURKOWICZ, MJ; KARPINSKA, J (2016). LC/MS/MS analysis of α‐tocopherol and coenzyme Q10 content in lyophilized royal jelly, beebread and drone homogenate. Journal of mass spectrometry, 51(11):1023-1029. 12 MATSUKA, M; WATABE, N; TAKEUCHI, K (1973). Analysİs of the food of larval drone honeybees. J Apic Res, 12(1): 3-7. 13 STAGACIU, S (1999). Apilarnil. Apitherapy course notes. P.520, Romania. 14 KOGALNİCEANU, S; LANCRAJAN, I; ARDELEAN, G (2010). Changes of the glucidic metabolism determined by the physical effort of the treatment with the Aslavital and Apilarnil. Arad Med J, 3:33-41. 15 SAWCZUK, R; KARPINSKA, J; MILLTYK, W (2019). What do we need to know about drone brood homogenate and what is known. J Ethnopharmacol 5(245): 111581. 16 CONSTANTIN, D. 1989. Rezultate obpinute in tratamentul cu apilarnil potent a tulburarilar de dinami camsexuale. Romanian Apicult, 10: 21. 17 ILIESIU NV (1980). Apilarnil. The 8th biologically active bee product. Apic Romania 12:4 18 AOAC Authors. Official methods of analysis nitrogen-free extract calculatiob: 100 minus (moisture+ ash+ protein+ fiber+fat)-item 93. Association of Analytical Communities, Gaithersburg, MD, 17 th edition, 2006, NFNAP: PROX. 19 JENSEN AB; EVANS, J; JONAS-LEVI A, BENJAMIN O; MARTINEZ I; DAHLE B; ROOS N; LECOCQ A; FOLEY K. (2019) Standard methods for Apis mellifera brood as human food, J Apic Res 58 (2):1-28, 20 HU, F; LI, Y (2001). Nutritive value and pharmacological actions of Italian worker bee larvae and pupae. In Proceedings of the 37th International Apicultural Congress, Apimondia, Durban. 21 MARGAOAN, R; MARGHITAS, LA; DEZMIREAN, DS; BOBIS, O; BONTA, V; CATANA, C; URCAN, A; MUREŞAN, CI; MARGIN, MG (2017). Comparative study on quality parameters of royal jelly, apilarnil and queen bee larvae triturate. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Anim Sci Biotechnol 74(1): 51-58. 22 LAZARYAN, DS (2002). Comparative amino acid analysis of bee brood. Pharm Chem J, 36(12): 680-682. 23 ÖZKÖK, D; SİLİCİ S (2017). Antioxidant activities of honeybee products and their mixtures. Food Sci Biotechnol 26(1): 201-206.