Expression of stem-cell pluripotency markers in amniotic fluid cells

Yıl 2019, Cilt: 12 Sayı: 3, 388 - 396, 31.12.2019

Mustafa Ertan Ay , Ezgi Çokaklı Murat Çokaklı Ümit Karakaş , Özlem İzci Ay , Hüseyin Durukan Mehmet Emin Erdal

https://doi.org/10.26559/mersinsbd.626035

Öz

Aim: As being a
heterogenous population but having different pools of self-renewing cells,
amniotic fluid cells (AFCs) have been considered as promising cell sources for
regenerative medicine owing to their multipotency and effective reprogramming
capacity. AFCs can more easily and effectively be
reprogrammed than terminally differentiated cells to generate induced
pluripotent stem cells (iPSCs) by transfectional and chemical strategies. But
stem cell dynamics and differentiation capacity of AFCs into multiple lineages
were poorly understood. The aim of this study was to determine the expression
and stem cell characteristics of stem cell pluripotency markers in amniotic
fluid cells. Methods: In this study,
the expression profile of pluripotency markers in AFCs taken from 17 patients
were investigated by conventional RT-PCR by qualitatively. Results: The results showed that all the
pluripotency markers (OCT3/OCT4, SOX2, KLF4, MYC,
KIT, NANOG, DPPA3, DPPA5, FUT4, SALL4) were expressed in AFCs from different
patients but UTF1 is shows a
non-specific expression. Conclusion:
Consequently, our results revealed that AFCs have a unique stem cell profile
and pluripotency markers may be impactful when choosing cells for reprogramming
and regenerative medicine applications. 

Anahtar Kelimeler

Amniotic fluid cells, pluripotency markers, expression, regenerative medicine

Amniyotik sıvı hücrelerinde kök hücre pluripotensi belirteçlerinin ifadesi

Öz

Amaç: Amniyotik sıvı farklı
seviyelerde kendini yenileyebilen hücre gruplarından oluşmaktadır ve bu
hücrelerin kök hücre özellikleri henüz tam olarak karakterize edilmemiştir. Son
yıllarda amniyotik sıvı hücreleri (AFC) rejenaratif tıp için umut veren kök hücre
kaynağı olarak görülmektedir. AFC’ler transfeksiyonel ve kimyasal stratejiler
ile indüklenmiş pluripotent kök hücre (iPSC)’ler oluşturmak için terminal
olarak farklılaşmış hücrelerden daha kolay ve etkili bir şekilde yeniden
programlanabilirler. Fakat AFC’lerin kök hücre potansiyelleri ve farklı hücre
soylarına farklılaşma kapasitesi tam olarak anlaşılamamıştır. Bu çalışmada amniyotik
sıvı hücrelerinde kök hücre pluripotensi belirteçlerinin ifadelerinin ve kök
hücre karakterlerinin belirlenmesi amaçlanmıştır.
Yöntem: Bu çalışmada, 17 amniyon
sıvısından elde edilen AFC’lerde pluripotensi belirteçlerinin ifade edilip
edilmediği geleneksel RT-PCR ile kalitatif olarak araştırılmıştır. Bulgular: Elde edilen sonuçlar, farklı
hastalardan alınan AFC örneklerinde incelenen pluripotensi belirteçlerinden OCT3/OCT4, SOX2, KLF4, MYC, KIT, NANOG,
DPPA3, DPPA5, FUT4, SALL4’ün ifade edildiğini, UTF1 için ise non-spesifik
ifade olduğunu göstermektedir.
Sonuç: Elde edilen bulgular doğrultusunda
AFC’nin kendine özgü bir kök hücre profiline sahip olduğu ve pluripotensi
belirteçlerinin yeniden programlama ve rejeneratif tıp uygulamaları için hücre
seçiminde etkili şekilde kullanılabileceği düşünülmektedir.

Anahtar Kelimeler

Amniyotik sıvı hücresi, pluripotensi belirteci, ekspresyon, rejeneratif tıp

Kaynakça

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