Karvakrol ilave edilmiş antimikrobiyal yumuşak protez astarının yırtılma ve çekme bağlanma dayanımı ve sertliği

Yıl 2022, Cilt: 15 Sayı: 2, 292 - 300, 31.08.2022

Ayşe Seda Ataol , Gulfem Ergun , Aysel Uğur , Nurdan Saraç , Tuba Baygar

https://doi.org/10.26559/mersinsbd.1070743

Öz

Amaç: Bu çalışmanın amacı, fenolik bir bileşik olan karvakrol (kekik yağı) ile antimikrobiyal özellik kazanmış yumuşak protez astarının protez kaide materyaline bağlanma dayanımı, yırtılma dayanımı ve sertliğini değerlendirmektir. Yöntem: Her mm3'e 0.5 mL karvakrol eklenen silikon esaslı yumuşak protez astarı kontrol grubu (karvakrol ilavesiz silikon esaslı yumuşak protez astarı) (n=10/alt grup başına) ile karşılaştırıldı. Tüm test gruplarında numunelerin yarısı distile suda (37ºC) tutulurken, diğer yarısına termal yaşlandırma (10000 termal döngü-1 hafta) uygulandı. Karvakrol ilave edilmiş ve edilmemiş yumuşak protez astarının yırtılma dayanımı evrensel bir test cihazında (Lloyd Universal Tester; AMETEK, Inc., Hampshire, İngiltere) 50 mm/dak'lık bir çapraz kafa hızında, çekme bağlanma dayanımı aynı cihaz ile 10 mm/dak çapraz kafa hızında ve sertliği dijital vikers sertlik ölçüm cihazında (HMV, Shimadzu, Tokyo, Japonya) test edildi. Aksi belirtilmedikçe, p<0.05 için sonuçlar istatistiksel olarak anlamlı kabul edildi. Ancak, olası tüm çoklu karşılaştırmalarda Tip I hatayı kontrol etmek için Bonferroni Düzeltmesi yapıldı. Bulgular: Çalışmamızdan elde edilen bulgular dahilinde, yumuşak protez astarına karvakrol ilavesi, test edilen yırtılma ve çekme bağlanma dayanımı üzerinde istatistiksel olarak anlamlı etkiler göstermedi (p>0.05). Ayrıca tüm test gruplarında kontrol grubuna göre istatistiksel olarak anlamlı bir sertlik artışına neden oldu (p<0.001). Termal yaşlandırma genel olarak mekanik özellikler üzerinde önemli bir etki göstermedi (p>0.05). Sonuç: Bu in vitro çalışma, yumuşak protez astarına antimikrobiyel etkinliği bulunan karvakrol ilavesinin malzemenin yırtılma ve çekme bağlanma dayanımına etki etmediğini, ancak sertliğini arttırdığını ortaya koymuştur.

Anahtar Kelimeler

Protez astarları, kekik bitkisi, yırtılmalar, gerilme direnci, sağlamlık testleri

Destekleyen Kurum

TÜBİTAK (Türkiye Bilimsel ve Teknolojik Araştırma Kurumu)

Proje Numarası

Proje No:118S856

Teşekkür

Bu çalışma TÜBİTAK (Türkiye Bilimsel ve Teknolojik Araştırma Kurumu) tarafından desteklenmiştir (Program kodu-1002/Proje No:118S856)

Tear and tensile bond strength and hardness of carvacrol added antimicrobial soft denture liner

Öz

Objective: The purpose of this study was to evaluate bonding strength to denture base material, tear strength and hardness of soft denture liner that gained antimicrobial properties with carvacrol (thyme oil) that is a phenolic compounds. Method: Silicone based soft denture liner added 0.5 mL of carvacrol per mm3 was compared to the control group (silicone based soft denture liner without carvacrol addition) (n=10/per subgroup). While half of the samples were kept in distilled water (37ºC) in all test groups, thermocycling (10000 thermal cycle-1 week) was applied to the other half. Tear strength of soft denture liner with and without carvacrol addition in a universal tester (Lloyd Universal Tester; AMETEK, Inc., Hampshire, UK) at a crosshead speed of 50 mm/min, tensile bond strength at 10 mm/min cross-section head speed with the same device and hardness were tested with a digital Vikers hardness tester (HMV, Shimadzu, Tokyo, Japan). Unless otherwise stated, results for p<0.05 were considered statistically significant. Bonferroni Correction was performed to control for Type I error in all possible multiple comparisons. Results: Within the findings obtained from our study, the addition of carvacrol to the soft denture liner did not show statistically significant effects on the tested tear and tensile bond strength (p>0.05). In addition, in all test groups, it caused a statistically significant increase in hardness compared to the control group (p<0.001). Thermal aging generally did not show a significant effect on mechanical properties (p>0.05). Conclusion: This in vitro study revealed that the addition of carvacrol, which has antimicrobial activity, to the soft denture liner did not affect the tear and tensile bond strength of the material, but increased its hardness.

Anahtar Kelimeler

Denture liners, thymus plant, tear, tensile strength, hardness tests

Proje Numarası

Proje No:118S856

Kaynakça

• Vojdani M, Zibaei M, Aar K, Zomorodian K, Ma R, Boshehri S. In- vitro study of the effect of clotrimazole incorporation into silicone soft liner on fungal colonization. Journal of Dentistry. 2009;9(1):19-23. doi:10.30476/DENTJODS.2019.43613
• Usta Kutlu I, Yanikoğlu ND, Kul E, Duymuş ZY, Sağsöz NP. Effect of sealer coating and storage methods on the surface roughness of soft liners. J Prosthet Dent. 2016;115(3):371-376. DOI: 10.1016/j.prosdent.2015.10.004
• Nam KY. In vitro antimicrobial effect of the tissue conditioner containing silver nanoparticles. J Adv Prosthodont. 2011;3(1):20. doi: 10.4047/jap.2011.3.1.20
• Vojdani M, Bagheri R, Khaledi AAR. Effects of aluminum oxide addition on the flexural strength, surface hardness, and roughness of heat-polymerized acrylic resin. J Dent Sci. 2012;7(3):238-244. DOI:10.1016/j.jds.2012.05.008
• Pachava KR, Nadendla LK, Alluri LSC, Tahseen H, Sajja NP. Invitro antifungal evaluation of denture soft liner incorporated with tea tree oil: a new therapeutic approach towards denture stomatitis. J Clin Diagnostic Res. 2015;9(6):62-64. doi: 10.7860/JCDR/2015/12396.6132
• Homayouni H, Kavoosi G, Nassiri SM. Physicochemical, antioxidant and antibacterial properties of dispersion made from tapioca and gelatinized tapioca starch incorporated with carvacrol. LWT - Food Sci Technol. 2017;77:503-509. doi:10.1016/j.lwt.2016.12.007
• Ben Arfa A, Combes S, Preziosi-Belloy L, Gontard N, Chalier P. Antimicrobial activity of carvacrol related to its chemical structure. Lett Appl Microbiol. 2006;43(2):149-154. DOI: 10.1111/j.1472-765X.2006.01938.x
• Alzate P, Miramont S, Flores S, Gerschenson L. Effect of the potassium sorbate and carvacrol addition on the properties and antimicrobial activity of tapioca starch - Hydroxypropyl methylcellulose edible films. Starch‐Stärke. 2017;69(5-6):1600261. doi:10.1002/star.201600261
• Baygar T, Ugur A, Sarac N, Balci U, Ergun G. Functional denture soft liner with antimicrobial and antibiofilm properties. J Dent Sci. 2018;13(3):213-219. doi: 10.1016/j.jds.2017.10.002
• Muttagi S, Subramanya JK. Effect of incorporating seed oils on the antifungal property, surface roughness, wettability, weight change, and glucose sorption of a soft liner. J Prosthet Dent. 2017;117(1):178-185. DOI: 10.1016/j.prosdent.2016.05.010
• Chladek G, Kasperski J, Barszczewska-Rybarek I, Zmudzki J. Sorption, solubility, bond strength and hardness of denture soft lining incorporated with silver nanoparticles. Int J Mol Sci. 2013;14(1):563-574. doi: 10.3390/ijms14010563
• Oguz S, Mutluay MM, Dogan OM, Bek B. Effect of thermocycling on tensile strength and tear resistance of four soft denture liners. Dent Mater J. 2007;26(2):296-302. doi: 10.4012/dmj.26.296.
• Chladek G, Mertas A, Barszczewska-Rybarek I, et al. Antifungal activity of denture soft lining material modified by silver nanoparticles-a pilot study. Int J Mol Sci. 2011;12(7):4735-4744. doi: 10.3390/ijms12074735
• ISO 10139-2-Dentistry-Soft Lining Materials for Removable Dentures-Part 2: Materials for Long-Term Use, 2009.
• Akin H, Tugut F, Mutaf B, Akin G, Ozdemir AK. Effect of different surface treatments on tensile bond strength of silicone-based soft denture liner. Lasers Med Sci. 2011;26(6):783-788. doi: 10.1007/s10103-010-0825-6.
• Jaboinski LT, Miranda ME, Tarkany R, et al. Effect of the addition of propolis on a soft denture liner on bond strength with an acrylic resin. J Heal Sci Inst. 2015;33(3):223-227.
• Sánchez-Aliaga A, Pellissari CVG, Arrais CAG, Michél MD, Neppelenbroek KH, Urban VM. Peel bond strength of soft lining materials with antifungal to a denture base acrylic resin. Dent Mater J. 2016;35(2):194-203. doi: 10.4012/dmj.2014-269.
• Rawat P, Agarwal S, Tripathi S. Effect of addition of antifungal agents on physical and biological properties of a tissue conditioner: an in-vitro study. Adv Pharm Bull. 2017;7(3):485-490. doi: 10.15171/apb.2017.059.
• Landayan JIA, Manaloto ACF, Lee JY, Shin SW. Effect of aging on tear strength and cytotoxicity of soft denture lining materials; in vitro. J Adv Prosthodont. 2014;6(2):115-120. doi: 10.4047/jap.2014.6.2.115
• Chladek G, Żmudzki J, Kasperski J. Long-term soft denture lining materials. Materials (Basel). 2014;7(8):5816-5842. doi: 10.3390/ma7085816.
• Badaró MM, Prates TP, Leite-Fernandes VMF, Oliveira V de C, Paranhos H de FO, Silva-Lovato CH. In vitro evaluation of resilient liner after brushing with conventional and experimental ricinus communis -based dentifrices. J Prosthodont. 2019:28:857–862. doi:10.1111/jopr.12680
• Mancuso DN, Goiato MC, Zuccolotti BCR, Moreno A, Dos Santos DM, Pesqueira AA. Effect of thermocycling on hardness, absorption, solubility and colour change of soft liners. Gerodontology. 2012;29(2):215-219. doi:10.1111/j.1741-2358.2010.00447.x