Ordu İli Kivi Üretim Alanlarındaki Toprak Kökenli Fungusların Tanımlanması ve Patojenisitesi

Yıl 2020, Cilt: 10 Sayı: 1, 58 - 65, 24.06.2020

Nusret Şahin , Muharrem Türkkan

Öz

Bu çalışma, Ordu ilindeki kivi bahçelerinde kök çürüklüğüne neden olan fungal hastalık etmenlerini tanımlamak ve onların patojenisitelerini belirlemek amacıyla yürütülmüştür. Bu amaçla, 2013 ve 2014 yıllarında, Ordu İli ticari kivi yetiştiriciliğinin yaklaşık %97’sini kapsayan Altınordu, Perşembe, Gülyalı, Fatsa, Ünye, İkizce, Ulubey, Kabadüz ve Çaybaşı ilçelerinden toplam 135 bahçede inceleme yapılmıştır. Çalışmada, kivi bahçelerindeki hastalıklı bitkilerden toplam 214 fungal izolat elde edilmiştir. İzolatların %37.38 (80 izolat)’inin Fusarium oxysporum’a, %10.75 (23 izolat)’inin F. solani’ye, %16.82 (36 izolat)’sinin Fusarium spp.’ye, %7.94 (17 izolat)’ünün BN Rhizoctonia’ya, %3.74 (8 izolat)’ünün MN R. solani’ye, %5.61 (12 izolat)’inin Pythium spp.’ye, %3.74 (8 izolat)’ünün Macrophomina phaseolina’ya, %2.80 (6 izolat)’inin Cylindrocarpon spp.’ye, %1.4 (3 izolat)’ünün Verticillium spp.’ye, %2.34 (5 izolat)’ünün Acremonium spp.’ye, %2.34 (5 izolat)’ünün Clonostachys spp.’ye, %1.87 (4 izolat)’sinin Rhizopus spp.’ye ve %3.27 (7 izolat)’sinin Trichoderma spp.’ye ait olduğu belirlenmiştir. İzolatların yaklaşık %13’ü kullanılarak kivi fideleri ile yürütülen patojenisite testlerinde, izolatların hastalık şiddeti skala değerleinin 0.67-5.0 arasında değiştiği tespit edilmiştir. Patojenisite testinde kullanılan izolatlardan, Cyb-1, İkz-3 ve İkz-4 (F. solani); AO-11 ve AO-12 (R. solani); ile Cyb-4 ve Üny-7 (Pythium spp.) en virülent izolatlar olarak belirlenmiştir. AO-4 (Clonostachys spp.), Ulu-11 (Rhizopus spp.) ve Prs-15 (Trichoderma spp.) izolatlarının hastalık şiddeti skala değerleri ve yukarıda belirtilen izolatların skala değerleri arasındaki farklılık istatistiksel olarak önemli olarak bulunmuştur (P<0.05). Ayrıca, F. solani, R. solani, Pythium spp., M. phaseolina ve Cylindrocarpon spp.’nin tüm izolatları ve F. oxysporum (Gül 1 ve Ulu-4), Fusarium spp. (Gül-2) ve BN Rhizoctonia (AO-13 ve Gül-8)’nın bazı izolatları kök uzunluğunu, kök yaş ve kuru ağırlıklarını kontrol bitkilerine kıyasla önemli ölçüde azaltmıştır (P<0.05).

Anahtar Kelimeler

Kivi, fungal kök çürüklüğü etmenleri, patojenisite

Destekleyen Kurum

Ordu Üniversitesi Bilimsel Araştırma Projeleri tarafından desteklenmiştir.

Proje Numarası

Proje No: TF-1306

Teşekkür

Bu çalışma, Ordu Üniversitesi Bilimsel Araştırma Projeleri tarafından desteklenmiştir (Proje No: TF-1306). Çalışmada kivilerden elde edilen kök çürüklüğü etmeni fungusların teşhisinde yardımcı olan Prof. Dr. Berna Tunalı ve Doç. Dr. İsmail Erper’e (Ondokuz Mayıs Üniversitesi Ziraat Fakültesi Bitki Koruma Bölümü, Atakum, Samsun)’e teşekkür ederiz.

Ordu İli Kivi Üretim Alanlarındaki Toprak Kökenli Fungusların Tanımlanması ve Patojenisitesi

Öz

This study was carried out in order to identify fungal diseases that cause root rot in kiwifruit orchards in Ordu province and to determine their pathogenicity. For this purpose, a total of 135 kiwifruit orchards in Altınordu, Perşembe, Gülyalı, Fatsa, Ünye, İkizce, Ulubey, Kabadüz and Çaybaşı districts, where contain approximately 97% of commercial kiwifruit cultivation of Ordu province, were investigated in 2013 and 2014. In the study, a total of 214 fungal isolates were obtained from the diseased plants in the kiwifruit orchards. It was determined that 37.38% (80 isolates) of the isolates belong to Fusarium oxysporum, 10.75% (23 isolates) to F. solani, 16.82% (36 isolates) to Fusarium spp., 7.94% (17 isolates) to BN Rhizoctonia, 3.74% (8 isolates) to MN R. solani, 5.61% (12 isolates) to Pythium spp., 3.74% (8 isolates) to Macrophomina phaseolina, 2.80% (6 isolates) to Cylindrocarpon spp., 1.4% (3 isolates) to Verticillium spp., 2.34% (5 isolates) to Acremonium spp., 2.34% (5 isolates) to Clonostachys spp., 1.87% (4 isolates) to Rhizopus spp. and 3.27% (7 isolates) to Trichoderma spp. In the pathogenicity tests carried out using approximately 13% of all the isolates on kiwifruit seedlings, it was found that the virulence of the isolates ranged between 0.67 to 5.0. Of the isolates used in this test, Cyb-1, İkz-3 and İkz-4 (F. solani); AO-11 and AO-12 (R. solani); and Cyb-4 and Üny-7 (Pythium spp.) were the most virulent isolates. The difference between the virulence of AO-4 (Clonostachys spp.), Ulu-11 (Rhizopus spp.) and Prs-15 (Trichoderma spp.) isolates, and that of the above-mentioned isolates was statistically significant (P<0.05). In addition, all isolates of F. solani, R. solani, Pythium spp., M. phaseolina and Cylindrocarpon spp. and some isolates of F. oxysporum (Gül 1 and Ulu-4), Fusarium spp. (Gül-2) and BN Rhizoctonia (AO-13 and Gül-8) significantly reduced root length, and root fresh and dry weights compared to control plants (P<0.05).

Anahtar Kelimeler

Kiwifruit, fungal root rot agents, pathogenicity

Proje Numarası

Proje No: TF-1306

Kaynakça

• Akıllı S, Serçe ÇU, Katırcıoğlu YK, Karakaya A, Maden S 2011. Involvement of Phytophthora citrophthora in kiwifruit decline in Turkey. Journal of Phytopathology 159:579-581.
• Anonymous 1996. International Course on the Identification of Fungi of Agricultural and Enviromental Significance. 11 August – 20 September, 1996, IMI, Egham, UK.
• Asan A 2011. Checklist of Fusarium species reported from Turkey. Mycotaxon 116(1): 479.
• Barnett HL 1960. Illustrated Genera of Imperfect Fungi. Department of Plant Pathology, Bacteriyology and Entomology West Virginia University Morgantown, West Virginia. p. 225.
• Baudry A, Morzieres JP, Ellis R 1991. Effect of Phytophthora spp. on kiwifruit in France. New Zealand Journal of Crop and Horticultural Science 19(4): 395-398.
• Booth C 1971. The genus Fusarium, Commonwealth Agricultural Bureaux, Kew, Surrey, England, 237.
• Brook PJ 1986. Diseases of kiwifruit. In ‘Kiwifruit: science and management’.(Eds IJ Warrington, GC Weston). New Zealand Ray Richards Publisher pp420-428.
• Burgess LW, Summerrell BA, Bullock S, Gott KP, Backhouse D 1994. Laboratory manual for Fusarium research (3rd Edition), USA, 388.
• Conn KE, Gubler WD, Mircetich SM, Hasey JK 1991. Pathogenicity and relative virulence of nine Phytophthora spp. from kiwifruit. Phytopathology 81(9): 974-979.
• Çiftçi O, Serçe ÇU, Türkölmez Ş, Derviş S 2016. First Report of Phytophthora palmivora causing crown and root rot of kiwifruit (Actinidia deliciosa) in Turkey. Plant Disease 100(1): 210.
• Dhingra OD, Sinclair JB 1973. Location of Macrophomina phaseolina on soybean plants related to culture characteristics and virulence. Phytopathology 63:934–936
• Di Marco S, Calzarano F, Osti F, Mazzullo A 2004. Pathogenicity of fungi associated with a decay of kiwifruit. Australasian Plant Pathology 33(3): 337-342.
• Ellis MB 1993a. Dematiaceous Hyphomycetes. Former Prencipal Mycologist Commonwealth Mycological Institute, Kew 7-594.
• Ellis MB 1993b. More Dematiaceous Hyphomycetes. Former Prencipal Mycologist Commonwealth Mycological Institute, Kew 7-489.
• Erper I, Tunali B, Agusti-Brisach C, Armengol J 2011. First report of Cylindrocarpon liriodendri on kiwifruit in Turkey. Plant Disease 95:76.
• Erper I, Agustí-Brisach C, Tunali B, Armengol J 2013. Characterization of root rot disease of kiwifruit in the Black Sea region of Turkey. European Journal of Plant Pathology 136(2): 291-300.
• FAO 2020. http://faostat3.fao.org/download/Q/QC/E (Erişim Tarihi: 10.01.2020).
• Grigorov SP 1974. Karantina na restaniata, Zemizdat, Sofya, 346p.
• Güncan A 2015. Current status of the kiwifruıt pests in Turkey. Acta Hortic. 1096, 371-376.
• Horner IJ 1985. How serious is the Armillaria problem? New Zealand Kiwifruit, December 1985: 20.
• Kurbetli İ, Ozan S 2013. Occurrence of Phytophthora root and stem rot of kiwifruit in Turkey. Journal Phytopathology 161(11-12): 887-889.
• Latorre BA, Alvarez C, Ribeiro OK 1991. Phytophthora root rot of kiwifruit in Chile. Plant Disease 75(9): 949-952.
• Lee YH, Jee HJ, Cha KH, Ko SJ, Park KB 2001. Occurrence of Phytophthora root rot on kiwifruit in Korea. The Plant Pathology Journal 17(3): 154-158.
• Mahdavi E 2013. Occurrence of Phytophthora root and collar rot disease of kiwifruit orchards in the west part of the Mazandaran Province. Scholarly Journal of Agricultural Science 3(8): 331-335.
• Nipoti P, Sandalo S, Prodi A, Credi R, Spada G, Graziani S 2003. An Unusual Wood Disease of Kiwifruit In Italy. International Society for Horticultural Science, V International Symposium on Kiwifruit.
• Polat Z, Awan QN, Hussain M, Akgül DS 2017. First report of Phytopythium vexans causing root and collar rot of kiwifruit in Turkey. Plant Disease 101(6): 1058. Sneh B, Burpee L, Ogoshi A 1991. Identification of Rhizoctonia species. St. Paul, MN: APS Press.
• Sneh B, Jabaji-Hare S, Neate S, Dijst G 1996. Rhizoctonia species: Taxonomy, molecular biology, ecology, pathology and diseases control. Dordrecht, The Netherlands: Kluwer Academic Press.
• Strik B, Cahn H, Buller G, Tiyayon C, Pescie M 2005. Growing kiwifruit. pacific northwest extension (The Oregon State University Extension Service, Washington State University Extension, and University of Idaho Extension), USA, 27 pp.
• Taheri H, Beygi F, Gol Mohammadi M, Aduli B 2007. Etiology of kiwifruit crown and root fungal pathogens in North of Iran. http://agris.fao.org/agris-search/search.do?recordID=IR2012001108.
• TÜİK 2012. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr-(ErişimTarihi: 01.01.2013).
• TÜİK 2018. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr-(ErişimTarihi: 10.05.2019).
• Türkkan M, Erper I, Kılıçoğlu MÇ, Yazıcıoğlu E, Özcan M 2018. Characterization and pathogenicity of Rhizoctonia spp. isolated from kiwifruit in the Middle and Eastern Black Sea region of Turkey. Journal of Phytopathology 166(11-12): 761-774.
• Türkkan M, Şahin N, Özer G, Evgin Z, Yaman M, Erper I 2020a. First report of Verticillium dahliae causing Verticillium wilt on kiwifruit in Ordu, Turkey. Journal of Plant Pathology 102:221–222.
• Türkkan M, Benli Hİ, Yılmaz Ö, Özer G, Yaman M, Şahin N, Erper I 2020b. First report of charcoal rot caused by Macrophomina phaseolina on kiwifruit in Turkey. Journal of Plant Pathology 102:535.
• Yajun H, Peikun Q 1998. Studies on the cause of root rot of kiwifruit in Guangdong Province. Journal of South China Agricultural University 19(4):19-22.
• Ying-Ying Y, Liang C, Hong-Hai Z 2017. The pathogen causing bower kiwifruit Fusarium root rot. Mycosystema 36(10): 1369-1375.
• Yonat H 2016. Ordu ili kivi bahçelerinde görülen yabancı ot türlerinin ve yoğunluklarının belirlenmesi Yüksek Lisans Tezi, Ordu Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı, Ordu.