The Relationship between IL-1β [-31 T/C] Gene Polymorphism and Symptomatic Lumbar Disc Herniation

Yıl 2023, Cilt: 10 Sayı: 1, 62 - 64, 10.04.2023

Veli Çıtışlı , Aylin Koseler

https://doi.org/10.47572/muskutd.1186191

Öz

In this study, the relationship between IL-1β [-31 C / T] gene polymorphisms in the herniated lumbar disc is offered to be investigated. IL-1β [-31 C / T] gene was amplified by the PCR [Polymerase Chain Reaction] method. Polymorphic site cut with the restriction enzyme Alu I and genotyping were performed. Fifteen subjects (15%) were homozygous for the C/C allele at -31 position of IL-1β gene. Of the remaining 85 subjects, 64 (64%) were heterozygous for C/T and 21 (21%) were homozygous for T/T. In LDH group, fifteen subjects (30%) were homozygous for the T/T allele at -31 position of IL-1β gene. 35 subjects were heterozygous for C/T (70%). There were significant differences between control and LDH group. In LDH group, C/T and T/T genotype were higher than the control group, however in control group C / C genotype was higher. C/C genotype was not determined in LDH group. In this study, IL-1β -31T allele is shown to be associated with the Lumbar herniated disc.

Anahtar Kelimeler

Genotype, IL-1β Polymorphism, Lumbar Disc Herniation

Destekleyen Kurum

The authors declared that this study received no financial support.

Semptomatik Lomber Disk Hernisi ile IL-1β [-31 T/C] Gen Polimorfizm İlişkisi

Öz

Bu çalışmada fıtıklaşmış lomber diskte IL-1β [-31 C/T] gen polimorfizmleri arasındaki ilişkinin araştırılması önerilmektedir. IL-1β [-31 C/T] geni PCR [Polimeraz Zincir Reaksiyonu] yöntemi ile amplifiye edildi. Alu I restriksiyon enzimi ile polimorfik site Cut ve genotiplendirme yapıldı. On beş denek (%15), IL-1β geninin -31 konumunda C/C aleli için homozigottu. Kalan 85 denekten 64'ü (%64) C/T için heterozigot ve 21'i (%21) T/T için homozigottu. LDH grubunda on beş denek (%30) IL-1β geninin -31 pozisyonunda T/T alleli için homozigottu. 35 denek C/T için heterozigottu (%70). Kontrol ve LDH grubu arasında önemli farklılıklar vardı. LDH grubunda C/T ve T/T genotipi kontrol grubunagöre daha yüksek, kontrol grubunda ise C/C genotipi daha yüksekti. LDH grubunda C/C genotipi belirlenmedi. Bu çalışmada IL-1β -31T allelinin Lomber herniye disk ile ilişkili olduğu gösterilmiştir

Anahtar Kelimeler

Genotip, IL-1β Polimorfizmi, Lomber Disk Hernisi

Kaynakça

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