Antigenotoxic Effect of Ascorbic Acid on Genotoxic Damage in Oreochromis niloticus Induced by Trifluralin, Treflan and Ethyl Methane Sulfonate

Yıl 2017, , 10 - 19, 28.07.2017

Serpil Könen Adıgüzel Tolga Çavaş

https://doi.org/10.17100/nevbiltek.331407

Öz

In this
study, possible anti-genotoxic effect of ascorbic acid (vitamin C) was
investigated by micronucleus and nuclear abnormality test in peripheral
erythrocytes of a commercially important fish species, Oreochromis niloticus, (Nile Tilapia). The fish were exposed to
genotoxic concentrations of trifluralin (10 µg/L), and its commercial form,
Treflan (10 µg/L, composition: trifluralin 480 g/L and
commercially-confidential components) and ethyl methane sulfonate (EMS) (as a
positive control, 10 mg/L) for 6 days. Trifluralin, treflan and EMS were
administered to living space (30x20x15cm aquarium and 10 liter tap water) of
fish. Tap water as negative control was used. Ascorbic acid (0.05 %) was
injected to the fish by intracoelomic route as 0.1 ml/10 g body weight at the 1^st
and 3^rd days of exposures to trifluralin. The genotoxic effects of
the herbicides and possible anti-genotoxic effect of ascorbic acid were
evaluated using the micronucleus test and nuclear abnormality. Analysis of the
results showed that applied concentrations of trifluralin, treflan and EMS   increased the frequency of micronucleated
cells. However, in the fish administrated ascorbic acid along with trifluralin,
treflan and EMS there was a significant decrease in percentage of micronuclei
as compared to trifluralin, treflan and EMS treated fish. In conclusion,
ascorbic acid ameliorated the genotoxicity by the herbicides and therefore we
may suggest the use of this reducing agent for the prevention of genotoxicity in
fish farming.

Anahtar Kelimeler

Antigenotoxicity, ascorbic acid, Micronucleus, Oreochromis niloticus, Trifluralin

Trifluralin,Treflan ve Etil Metan Sülfonatın Oreochromis niloticus’ta Oluşturduğu Genotoksik Hasar Üzerine Askorbik Asitin Antigenotoksik Etkisi

Öz

Bu çalışmada askorbik asitin (vitamin C)
muhtemel antigenotoksik etkisi, ticari değeri olan balık türü Oreochromis niloticus (Nile Tilapia)’un
eritrositlerinde mikronukleus ve nuklear anomali testleri kullanılarak
değerlendirilmiştir. Balıklar; herbisit etken maddesi trifluralin ve ticari
formu treflanın (litrede 480 gr trifluralin ve ticari olarak özel bileşenler)
genotoksik konsantrasyonlarına (10 µg/L), pozitif kontrol olarak etil metan
sülfonata (EMS) (10 mg/L) 6 gün boyunca maruz bırakılmıştır. Trifluralin, treflan
ve etil metan sülfonat konsantrasyonları, içerisinde 10 litre musluk suyu
bulunan 30x20x15cm ebatlarındaki akvaryumlara eklenmiştir. Musluk suyu negatif
kontrol olarak kullanılmıştır. Askorbik asit (%0.05) balıklara intraperitonal
olarak maruziyetin 1. ve 3. günlerinde 0.1ml/10g vücut ağırlığı olacak şekilde
uygulanmıştır. Askorbik asitin herbisitlerin oluşturduğu genotoksik hasarı
düzenleyici etkisi mikronukleus ve nuklear anomali testi ile
değerlendirilmiştir. Analiz sonuçları; trifluralin, treflan ve etil metan
sülfonat uygulamalarının balık eritrositlerinde genotoksik hasarı yükselttiğini
göstermiştir. Ancak, kimyasallarla kombine şekilde askorbik asit uygulaması
hasarlı hücre oranını önemli derecede azaltmıştır. Sonuç olarak, askorbik asit
herbisitler tarafından oluşturulan genotoksik hasara karşı koruyucu etki
göstermiştir. Bu nedenle balık çiftliklerinde genotoksik hasarın
önlenmesi/azaltılması amacıyla yemlerde askorbik asit kullanılabilir.

Anahtar Kelimeler

Antigenotoksik, askorbik asit, mikronukleus, Oreochromis niloticus, Trifluralin

Kaynakça

• Fetoui H., Makni M., Garoui E.M., Zeghal N., “Toxic effects of lambda-cyhalothrin, a synthetic pyrethroid pesticide, on the rat kidney: Involvement of oxidative stress and protective role of ascorbic acid” Experimental and Toxicologic Pathology, 62, 593–599, 2010
• Ghiazza G., Zavarise G., Lanero M., Ferraro G., “Sister chromatid exchanges induced in human lymphocyte chromosomes by trifluralin, atrazine and simazine” Bollettino Della Societa Italiana di Biologia Sperimentale, 11, 2149-2153, 1984
• Pilinskaia M.A., “ Evaluation of the cytogenetic effect of the herbicide treflan and of a number of its metabolites on mammalian somatic cells” Tsitologia Genetica, 21(2), 131-5, 1987
• Könen S., Cavas T., “ Genotoxicity testing of the herbicide trifluralin and its commercial formulation Treflan using the piscine micronucleus test” Environmental Molecular Mutagenesis, 49, 434-438, 2008
• Hooftman R.N., “The induction of chromosome-aberrations in Notobranchius rachowi (Pisces, Cypridontidae) after treatment with ethyl methane sulfonate or benzo[a]pyrene” Mutation Research , 91(4- 5), 347-352, 1981
• Guha B., Khuda-Bukhsh A.R., “ Efficacy of vitamin-C (L-ascorbic acid) in reducing genotoxicity in fish (Oreochromis mossambicus) induced by ethyl methane sulphonate” Chemosphere, 47, 49–56, 2002
• Ames B., “ Dietary carcinogens and anticarcinogens” Science, 221, 1256-63, 1983
• Block G., “Vitamin C and cancer prevention: The epidemiologicalevidence” American Journa of Clinical Nutrition, 53, 270-80, 1991
• Byers T., Perry G., “ Dietary carotenes, vitamin C and vitamin E as protective antioxidant in human cancer” Annual Review of Nutrition, 12, 139-59, 1992
• Kaya B., “ Antigenotoxic effect of Ascorbic acid on mutagenic dose of three alkylating agents” Turk Journal Biology, 27, 241-46, 2003