Determination of Oxidative Stress, Vitamin A, E, C and β-Carotene Levels in the Tissues of Luciobarbus esocinus (Heckel, 1843)

Yıl 2017, , 476 - 482, 30.12.2017

Sibel Barata Hülya Şahin Sibel Köprücü

https://doi.org/10.17100/nevbiltek.358601

Öz

In the study was
investigated the levels
of lipid peroxidation (malondialdehyde;
MDA) as oxidative stress, vitamin E (VE), A (VA), C (VC) and β-carotene (βC) in
liver, spleen, muscle and kidney tissues of Luciobarbus esocinus. The fish samples used in this study was catched from
Keban Dam Lake (Elazıg-Turkey) at August. Analyzes were
made in High Performance Liquid Chromotograph (HPLC). The
data were analyzed with an Independent-Sample T Test in SPSS 21. It
was determined that the
levels of parameters analysed was generally higher in liver and spleen compared to
kidney and muscle of female and male. The VE, VC and βC levels in liver and kidney were lower in
female than male. The levels of VC in spleen were higher in male according to
female. The VA level in muscle was lower in male than female, but the level of βC in this tissues was higher in male according to
female. Moreover, the MDA level wasn’t different according to sex.

Anahtar Kelimeler

Luciobarbus esocinus, Vitamins, Oxidative stress, Carotene

Luciobarbus esocinus (Heckel, 1843)’un Dokularındaki Oksidatif Stress, Vitamin A, E, C ve β-Karoten SeviyelerininTespiti

Öz

Bu çalışmada Luciobarbus
esocinus’un karaciğer,
dalak, kas ve böbrek dokularında oksidatif stres olarak lipid peroksidasyon
(malondialdehit-MDA), vitamin
E (VE), A (VA), C (VC) ve β-karoten (βC) seviyeleri araştırıldı. Çalışmada
kullanılan balık örnekleri Ağustos ayında Keban Baraj Gölü’nden yakalandı.
Analizler yüksek performanslı likit kromotografisinde yapıldı (HPLC). Veriler
SPSS 21’deIndependent-Sample T and Duncan Testi’nde analiz
edildi. Genel olarak analiz yapılan parametrelerin düzeyleri erkek ve dişilerin
böbrek ve kasına göre karaciğer ve dalağında daha yüksekti. Karaciğer ve
böbrekteki VE, VC ve βC
seviyeleri erkeklere göre dişilerde daha düşüktü. Dalaktaki VC seviyesi dişiye
göre erkekte daha yüksekti. Kastaki VA seviyesi dişiye göre erkeklerde daha
düşüktü. Fakat bu dokudaki βC’nin seviyesi dişiye göre erkekte daha yüksekti.
Bunlara ilave olarak bütün dokulardaki MDA seviyesi cinsiyete göre farklılık
göstermedi.

Anahtar Kelimeler

Luciobarbus esocinus, Vitamin, Oksidatif Stres, Karoten

Kaynakça

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