Atık Sulardan İzole Edilen Pseudomonas Spp.’ lerin Ekzopolisakkarit Üretimine Bazı Ağır Metallerin Etkisi

Yıl 2018, , 223 - 239, 30.12.2018

Süleyman Yalçın , Şahlan Öztürk , Berrin Keloğlu

https://doi.org/10.17100/nevbiltek.402196

Öz

Bu çalışmada atık su arıtma
havuzlarından alınan örneklerden 50 adet Pseudomonas
spp. izole edilmiştir. İzolatların; koloni morfolojileri, Gram boyama
sonuçları ve VITEK 2 Compact 30 (biomerieux) cihazında yapılan tür
tanımlamaları sonucunda 27 adet Pseudomonas
aeruginosa ve 23 adet P. stutzeri
oldukları tespit edilmiştir. Tanımlanan izolatlar 10 ppm Cr(VI), Cd(II), Cu(II)
ve Mn(II) konsantrasyonlarına maruz bırakılarak bu metallere karşı canlılıkları
tespit edilmiş ve her metal için canlılığını en çok koruyan izolatlar
seçilmiştir. Aynı zamanda izolatların canlılık değerlerine göre uygulanacak metallerin
4 farklı derişimi belirlenmiştir. Seçilen izolatlara belirlenen metal
konsantrasyonları verilerek yüzde ölüm değerleri tespit edilmiş ve LC50
değerleri hesaplanmıştır. Her metal için seçilen izolatlar, belirlenen
konsantrasyonlarda metale maruz bırakılarak EPS izolasyonu gerçekleştrilmiştir.
15 ppm Cr(VI) konsantrasyonunda en yüksek EPS üretiminin P.stutzeri S46 izolatında (74,68 mg/L), en düşük EPS üretiminin ise
P.stutzeri S18 izolatında olduğu
(39,37 mg/L) tespit edilmiştir. 40 ppm Cd(II) konsantrasyonunda en yüksek EPS
üretiminin P.stutzeri S23 izolatında
(60,93 mg/L), en düşük EPS üretiminin ise P.aeruginosa
S10 izolatında olduğu (40,93 mg/L) belirlenmiştir. 80 ppm Cu(II)
konsantrasyonunda en yüksek EPS üretiminin P.stutzeri
S45 izolatında (74,06 mg/L), en düşük EPS üretiminin ise P. aeruginosa S8 izolatında olduğu (28,28 mg/L) görülmüştür. 400
ppm Mn(II) konsantrasyonunda en yüksek EPS üretiminin P.stutzeri S44 izolatında (46,40 mg/L), en düşük EPS üretiminin ise
P.stutzeri S50 izolatında olduğu
(26,56 mg/L) tespit edilmiştir. Sonuç olarak artan metal dozlarına maruz kalan Pseudomonas spp. izolatlarının
ürettikleri EPS miktarlarında da artış olduğu tespit edilmiştir. Bu çalışma ile
atık sulardan izole edilen Pseudomonas
spp. izolatlarının bazı ağır metallerin EPS üretimine etkisi biyoteknolojik
açıdan vurgulanmıştır.

Anahtar Kelimeler

Pseudomonas aeruginosa, Pseudomonas stutzeri, Ağır metal

Circular Environmental Policies in The Industrial Production

Öz

In this study, 50 Pseudomonas
spp. have been isolated from the instances of waste water purification
pool. 27 Pseudomonas aeruginosa and
23 Pseudomonas stutzeri strains were
detected according to colony morphologies, Gram staining and type definitions
that have been made in the vitek 2 compact 30 (biomerieux) device. Identified
strains were exposed by 10 ppm Cr(VI), Cd(II), Cu(II) and Mn(II) concentrations
and detected their viabilities against these metals and by this way determined
the most resistant strains for each metal. Also, four different concentrations
of these metals were determined according to viability of strains. % death
values of strains were determined by treated with
metal concentrations and LC₅₀ values of metals were calculated.
Strains selected for each metal by exposing them to the specified
concentrations, EPS isolation has been carried out. In Cr(VI) concentration it
has been found that the Pseudomonas
stutzeri of the highest EPS production is S46 strains (74.68 mg/L) while
the lowest Pseudomonas stutzeri is
S18 (39.37 mg/L). In Cd(II) concentration; the Pseudomonas stutzeri of the highest EPS production is S23 (60.93
mg/L), while the lowest EPS production is Pseudomonas
aeruginosa S10 strains (40.93 mg/L). In Cu(II) concentration, the Pseudomonas stutzeri of the highest EPS
production is S45 strains (76.06 mg/L) while the lowest EPS production is Pseudomonas aeruginosa S8 strains (28.28
mg/L). In Mn (II) concentration, the Pseudomonas
stutzeri of the highest EPS production is S44 strains (46.40 mg/L), while
the Pseudomonas stutzeri of the
lowest EPS production is S50 strains (26.56 mg/L). As a result, exposed to
increasing metal dose of Pseudomonas spp.,
the amount of EPS produced by the strains has been found to be increased. By
this study it has been expressed the effect of some heavy metals of Pseudomonas spp strains, which have been
isolated from wastewater, to the EPS production in terms of biotechnology.

Anahtar Kelimeler

Pseudomonas aeruginosa, Pseudomonas stutzeri, Heavy metal, Exopolysaccharide.

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