IL-10 and IL-17 Gene Polymorphisms in Adult Idiopathic Thrombocytopenic Purpura

Yıl 2018, Cilt: 6 Sayı: 2, 36 - 44, 12.09.2018

Tuğba Songül Tat , Mustafa Yılmaz Ahmet Alver , Gülşah Bayçelebi

Öz

Objective: Interleukin 10 (IL-10) and
interleukin 17 (il-17) cytokine gene polymorphisms were investigated in many
autoimmune diseases and there were significant results in some studies but
there were a few published studies on IL-10 and il-17 gene polymorphisms in idiopathic
thrombocytopenic purpura ( ITP). In this study, we aimed to evaluate the IL-10 (-592 A/C) and IL-17
(A126G) gene polymorphisms and their serum levels in adult patients with ITP.

Materials and Methods: The IL-10 (-592
A/C) and IL-17 (A126G) gene polymorphisms in 50 patients with ITP and 51
healthy control groups were determined after DNA isolation and serum levels of
these cytokines were measured by ELISA method in 32 patients with ITP and 32
healthy volunteers.

Results: There was no statistically
significant difference in IL-10 (-592 A/C) and IL-17 (A126G) gene polymorphisms
between the patients and control groups, whereas serum IL-10 levels were found
to be statistically significant higher in ITP patients than control group
(p=0.003). Serum IL-17 levels were similar in patients
and control groups. In addition, age, sex, response to treatment and platelet
values at diagnosis were compared between the groups, but there was no
statistically significant difference between the groups.

Conclusion: In our study, IL-10 (-592
A/C) and IL-17 (A126G) gene polymorphisms were not found to be different in
patients with ITP compared to healthy subjects, although IL-10 levels were
found to be higher in the ITP group than healthy group. When we look the datas in the literature, we think that more
studies with larger series are needed in this field in order to clarify the
importance of cytokines in the diagnosis and follow-up of ITP.

Anahtar Kelimeler

Immune thrombocytopenic purpura, interleukin-10, interleukin -17

Erişkin İdiyopatik Trombositopenik Purpuralı Hastalarda IL-10 ve IL-17 Gen Polimorfizm

Öz

Amaç: İnterlökin 10 (IL-10) ve interlökin 17 (IL-17) sitokin
gen polimorfizmlerinin otoimmün birçok hastalıkta araştırıldığı ve bazılarında
anlamlı sonuçlar bulunduğu ancak idiyopatik
trombositopenik purpuralı
(ITP) hastalarda IL-10 ve IL-17 gen polimorfizmleri ile ilgili yayınlanmış az sayıda çalışma mevcut olduğu görülmüştür. Bu çalışmada erişkin ITP hastalarında IL-10 (-592 A/C) ve IL-17 (A126G) gen
polimorfizmi ve bu sitokinlerin serum düzeylerinin
değerlendirilmesi amaçlanmıştır.

Materyal
ve Metot: 50 ITP’ li hasta ve 51 sağlıklı kontrol
grubunda IL-10 (-592 A/C) ve IL-17 (A126G) gen polimorfizmleri, DNA izolasyonu
yapıldıktan sonra belirlenmiş ve bu sitokinlerin serum düzeyleri ELİSA yöntemi
ile toplam 32 ITP’li hastada ve 32 sağlıklı kişide ölçülmüştür.

Bulgular: Hasta
ve kontrol grupları arasında IL-10 (-592 A/C) ve IL-17 (A126G) gen
polimorfizmleri dağılımı açısından istatistiksel olarak anlamlı fark
bulunmazken, serum IL-10 düzeyleri ITP’li hastalarda sağlıklı bireylere göre
istatistiksel olarak anlamlı derecede yüksek bulunmuştur (p=0,003). Serum IL-17
düzeyleri ise hasta ve kontrol grubunda benzer bulunmuştur. Ayrıca gruplar
arasında yaş, cinsiyet, tedavi cevabı, tanı anıdaki trombosit değerleri
karşılaştırılmış ancak gruplar arasında istatistiksel olarak anlamlı fark
saptanmamıştır.

Sonuç: Çalışmamızda ITP’li hastalarda sağlıklı kişilere göre IL-10 (-592
A/C) ve IL-17 (A126G) gen polimorfizmleri sıklıklarının farklı olduğu
saptanamasa da IL-10 düzeyleri ITP’li grupta yüksek bulunmuştur. Sitokinlerin
ITP’nin tanı ve takibindeki önemini açıklığa kavuşturabilmek için literatürdeki
veriler de dikkate alındığında bu alanda daha geniş hasta serili çalışmalara
ihtiyaç olduğunu düşünmekteyiz. 

Anahtar Kelimeler

İmmun trombositopenik purpura, İnterlökin-10, İnterlökin -17

Kaynakça

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