Araştırma Makalesi
A Study on the Effects of Direct Factor Xa Inhibitors and Direct Thrombin Inhibitors on Human Primary Chondrocyte Cultures.
Öz
Anahtar Kelimeler
Apixaban; Chondrocyte; Dabigatran; Extracellular matrix; Rivaroxaban.
Destekleyen Kurum
None
Proje Numarası
None
Teşekkür
None
Kaynakça
- 1. Ozer N. Clinical studies conducted with new oral anticoagulants in atrial fibrillation: Which oral anticoagulant can be considered for which case in light of the clinical studies? Turk Kardiyol Dern Ars 2016;44 Suppl 2:33-40.
- 2. Deveci OS, Demir M, Aksoy M. Use of dabigatran in patients with non-valvular atrial fibrillation: answers to frequently asked questions. Arch Turk Soc Cardiol 2013;41 Suppl 4:25-33.
- 3. Bas DF, Topcuoglu MA, Arsava EM. Atrial fibrillation and stroke in the perspective of new oral anticoagulants. Turkish Journal of Cerebrovascular Diseases 2013;19(2):35-45.
- 4. Kaplan N, Karaarslan N, Yilmaz I, Sirin DY, Akgun FS, Caliskan T, et al. Are intervertebral disc tissue cells damaged when attempting to prevent thrombus formation using dabigatran, a new oral anticoagulant? Turk Neurosurg 2019;29(4):470-477.
- 5. Coleman CI, Peacock WF, Bunz TJ, Alberts MJ. Effectiveness and safety of apixaban, dabigatran, and rivaroxaban versus warfarin in patients with nonvalvular atrial fibrillation and previous stroke or transient ischemic attack. Stroke 2017;48(8):2142-2149.
- 6. Torrejon Torres R, Saunders R, Ho KM. A comparative cost-effectiveness analysis of mechanical and pharmacological VTE prophylaxis after lower limb arthroplasty in Australia. J Orthop Surg Res 2019;14(1):93.
- 7. Isyar M, Yilmaz I, Yasar Sirin D, Yalcin S, Guler O, Mahirogullari M. A practical way to prepare primer human chondrocyte culture. J Orthop 2016;13(3):162-7.
- 8. Gumustas SA, Yilmaz I, Isyar M, Sirin DY, Batmaz AG, Ugras AA, et al. Assessing the negative impact of phenyl alkanoic acid derivative, a frequently prescribed drug for the suppression of pain and inflammation, on the differentiation and proliferation of chondrocytes. J Orthop Surg Res 2016;11(1):70.
- 9. Guzelant AY, Isyar M, Yilmaz I, Sirin DY, Cakmak S, Mahirogullari M. Are chondrocytes damaged when rheumatologic inflammation is suppressed? Drug Chem Toxicol 2017;40(1):13-23.
- 10. Gumustas F, Yilmaz I, Sirin DY, Gumustas SA, Batmaz AG, Isyar M, et al. Chondrocyte proliferation, viability and differentiation is declined following administration of methylphenidate utilized for the treatment of attention-deficit/hyperactivity disorder. Hum Exp Toxicol 2017;36(9):981-992.
- 11. Karaarslan N, Yilmaz I, Sirin DY, Ozbek H, Kaya YE, Akyuva Y, et al. Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1? Ann Med Res 2018;25(4):756-62.
- 12. Sirin DY, Kaplan N, Yilmaz I, Karaarslan N, Ozbek H, Akyuva Y, et al. The association between different molecular weights of hyaluronic acid and CHAD, HIF-1α, COL2A1 expression in chondrocyte cultures. Exp Ther Med 2018;15(5):4205-4212.
- 13. Karaarslan N, Batmaz AG, Yilmaz I, Ozbek H, Caliskan T, Yasar Sirin D, et al. Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue. Exp Ther Med 2018;16(3):1647-1654.
- 14. Sirin DY, Karaarslan N. Evaluation of the effects of pregabalin on chondrocyte proliferation and CHAD, HIF-1α, and COL2A1 gene expression. Arch Med Sci 2018;14(6):1340-1347.
- 15. Kaplan N, Yilmaz I, Karaarslan N, Kaya YE, Sirin DY, Ozbek H. Does nimodipine, a selective calcium channel blocker, impair chondrocyte proliferation or damage extracellular matrix structures? Curr Pharm Biotechnol 2019;20(6):517-524.
- 16. Punzi L, Oliviero F, Plebani M. New biochemical insights into the pathogenesis of osteoarthritis and the role of laboratory investigations in clinical assessment. Crit Rev Clin Lab Sci 2005;42(4):279-309.
- 17. Boeth H, Raffalt PC, MacMahon A, Poole AR, Eckstein F, Wirth W, et al. Association between changes in molecular biomarkers of cartilage matrix turnover and changes in knee articular cartilage: a longitudinal pilot study. J Exp Orthop 2019;6(1):19.
- 18. Tao Y, Qiu X, Xu C, Sun B, Shi C. Expression and correlation of matrix metalloproteinase-7 and interleukin-15 in human osteoarthritis. Int J Clin Exp PAthol 2015;8(8):9112-9118.
- 19. Chang ZK, Meng FG, Zhang ZQ, Mao GP, Huang ZY, Liao WM, et al. MicroRNA-193b-3p regulates matrix metalloproteinase 19 expression in interleukin-1β-induced human chondrocytes. J Cell Biochem 2018;119(6):4775-4782.
- 20. Papathanasopoulos A, Kouroupis D, Henshaw K, McGonagle D, Jones EA, Giannoudis PV. Effects of antithrombotic drugs fondaparinux and tinzaparin on in vitro proliferation and osteogenic and chondrogenic differentiation of bone-derived mesenchymal stem cells. J Orthop Res 2011;29(9):1327-35.
Direkt Faktör Xa İnhibitörleri ve Direkt Trombin İnhibitörlerinin İnsan Primer Kondrosit Kültürlerine Etkileri Üzerine Bir Çalışma.
Öz
Amaç: Bu
çalışmada; direk faktör Xa inhibitörü apiksaban ve rivaroxaban ile
Direk/selektif olarak thrombin inhibitörü olan dabigatranın, primer insan
kıkırdak hücre kültürleri üzerine etkisinin gözlenmesi amaçlandı.
Gereç ve Yöntem: Kondrosit kültürleri monolayer olarak çoğaltılldı. Bu
kültürler üzerine apiksaban, dabigatran ve rivaroksaban farmasötik ajanları
ilave edildi. İlaç uygulanmayan hücre kültürleri control grubu olarak
kullanıldı. Hücrelere ait yüzey morfolojisi invert ışık mikroskobisi ile
değerlendirildi. Hücrelerin sağlıklı olup olmadığı ve proliferasyonlarının
devam edip etmediği MTT analizi ile spektrofotometrik olarak belirlendi ayrıca
AO/PI fluresan boyamalardan da yararlanılarak apoptotik hücre ölümü varlığı
araştırıldı. Kıkırdak oligo matriks protein (COMP), matriks metalloproteinaz
(MMP)-7 ve MMP-19 genlerine ait ifadeler ise quantitative real time polymerase
chain reaction (qRT-PCR) ile test edildi. Tüm analizler 21 gün boyunca belli
zaman aralıklarında gerçekleştirildi. Elde edilen veriler istatistiksel olarak
değerlendirildi ve sonuçlar raporlandı.
Sonuç: Bu üç farmakolojik ajanın, hem
hücre canlılığı ve proliferasyonunu hem de COMP, MMP-7 ve MMP-19 genlerine ait
ifadeleri değiştirdiği ve bu sonuçların istatistiksel olarak anlamlı olduğu
kaydedildi (P<0.05).
Çıkarım: Her
ne kadar da in-vitro deneylerden elde edilen sonuçlar
klinik uygulamaları tam olarak yansıtmıyor olsa bile, herhangi bir hastalığı
önleyebilmek amacı ile masumca uygulanan
ilaçların, kondrosit proliferasyonunu baskılayabileceği ve/veya ekstraselüler
matrix (ECM) yapısına zarar verebileceği gerçeği akıllarda tutulmalıdır.
Anahtar Kelimeler
Proje Numarası
None
Kaynakça
- 1. Ozer N. Clinical studies conducted with new oral anticoagulants in atrial fibrillation: Which oral anticoagulant can be considered for which case in light of the clinical studies? Turk Kardiyol Dern Ars 2016;44 Suppl 2:33-40.
- 2. Deveci OS, Demir M, Aksoy M. Use of dabigatran in patients with non-valvular atrial fibrillation: answers to frequently asked questions. Arch Turk Soc Cardiol 2013;41 Suppl 4:25-33.
- 3. Bas DF, Topcuoglu MA, Arsava EM. Atrial fibrillation and stroke in the perspective of new oral anticoagulants. Turkish Journal of Cerebrovascular Diseases 2013;19(2):35-45.
- 4. Kaplan N, Karaarslan N, Yilmaz I, Sirin DY, Akgun FS, Caliskan T, et al. Are intervertebral disc tissue cells damaged when attempting to prevent thrombus formation using dabigatran, a new oral anticoagulant? Turk Neurosurg 2019;29(4):470-477.
- 5. Coleman CI, Peacock WF, Bunz TJ, Alberts MJ. Effectiveness and safety of apixaban, dabigatran, and rivaroxaban versus warfarin in patients with nonvalvular atrial fibrillation and previous stroke or transient ischemic attack. Stroke 2017;48(8):2142-2149.
- 6. Torrejon Torres R, Saunders R, Ho KM. A comparative cost-effectiveness analysis of mechanical and pharmacological VTE prophylaxis after lower limb arthroplasty in Australia. J Orthop Surg Res 2019;14(1):93.
- 7. Isyar M, Yilmaz I, Yasar Sirin D, Yalcin S, Guler O, Mahirogullari M. A practical way to prepare primer human chondrocyte culture. J Orthop 2016;13(3):162-7.
- 8. Gumustas SA, Yilmaz I, Isyar M, Sirin DY, Batmaz AG, Ugras AA, et al. Assessing the negative impact of phenyl alkanoic acid derivative, a frequently prescribed drug for the suppression of pain and inflammation, on the differentiation and proliferation of chondrocytes. J Orthop Surg Res 2016;11(1):70.
- 9. Guzelant AY, Isyar M, Yilmaz I, Sirin DY, Cakmak S, Mahirogullari M. Are chondrocytes damaged when rheumatologic inflammation is suppressed? Drug Chem Toxicol 2017;40(1):13-23.
- 10. Gumustas F, Yilmaz I, Sirin DY, Gumustas SA, Batmaz AG, Isyar M, et al. Chondrocyte proliferation, viability and differentiation is declined following administration of methylphenidate utilized for the treatment of attention-deficit/hyperactivity disorder. Hum Exp Toxicol 2017;36(9):981-992.
- 11. Karaarslan N, Yilmaz I, Sirin DY, Ozbek H, Kaya YE, Akyuva Y, et al. Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1? Ann Med Res 2018;25(4):756-62.
- 12. Sirin DY, Kaplan N, Yilmaz I, Karaarslan N, Ozbek H, Akyuva Y, et al. The association between different molecular weights of hyaluronic acid and CHAD, HIF-1α, COL2A1 expression in chondrocyte cultures. Exp Ther Med 2018;15(5):4205-4212.
- 13. Karaarslan N, Batmaz AG, Yilmaz I, Ozbek H, Caliskan T, Yasar Sirin D, et al. Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue. Exp Ther Med 2018;16(3):1647-1654.
- 14. Sirin DY, Karaarslan N. Evaluation of the effects of pregabalin on chondrocyte proliferation and CHAD, HIF-1α, and COL2A1 gene expression. Arch Med Sci 2018;14(6):1340-1347.
- 15. Kaplan N, Yilmaz I, Karaarslan N, Kaya YE, Sirin DY, Ozbek H. Does nimodipine, a selective calcium channel blocker, impair chondrocyte proliferation or damage extracellular matrix structures? Curr Pharm Biotechnol 2019;20(6):517-524.
- 16. Punzi L, Oliviero F, Plebani M. New biochemical insights into the pathogenesis of osteoarthritis and the role of laboratory investigations in clinical assessment. Crit Rev Clin Lab Sci 2005;42(4):279-309.
- 17. Boeth H, Raffalt PC, MacMahon A, Poole AR, Eckstein F, Wirth W, et al. Association between changes in molecular biomarkers of cartilage matrix turnover and changes in knee articular cartilage: a longitudinal pilot study. J Exp Orthop 2019;6(1):19.
- 18. Tao Y, Qiu X, Xu C, Sun B, Shi C. Expression and correlation of matrix metalloproteinase-7 and interleukin-15 in human osteoarthritis. Int J Clin Exp PAthol 2015;8(8):9112-9118.
- 19. Chang ZK, Meng FG, Zhang ZQ, Mao GP, Huang ZY, Liao WM, et al. MicroRNA-193b-3p regulates matrix metalloproteinase 19 expression in interleukin-1β-induced human chondrocytes. J Cell Biochem 2018;119(6):4775-4782.
- 20. Papathanasopoulos A, Kouroupis D, Henshaw K, McGonagle D, Jones EA, Giannoudis PV. Effects of antithrombotic drugs fondaparinux and tinzaparin on in vitro proliferation and osteogenic and chondrogenic differentiation of bone-derived mesenchymal stem cells. J Orthop Res 2011;29(9):1327-35.
Toplam 20 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Proje Numarası | None |
| Yayımlanma Tarihi | 18 Aralık 2019 |
| Yayımlandığı Sayı | Yıl 2019 Cilt: 7 Sayı: 3 |
