Hydrogen peroxide-induced oxidative stress and apoptosis in SH-SY5Y cells: Protective effect of Momordica charantia fruit extract

Year 2023, Volume: 40 Issue: 3, 497 - 501, 30.09.2023

Handan Deligöz Ahmet Cumaoğlu

Abstract

Oxidative stress triggers apoptosis in neuronal cells, resulting in cellular loss, which is critical in the pathogenesis of neurodegenerative diseases. Momordica charantia (MC), a traditional medicinal plant, is famous for its variety of health benefits, including its anti-diabetic, anti-inflammatory, and antioxidant properties. The purpose of this study was to investigate on how MC might affect oxidative stress and apoptosis caused by H2O2. First, we investigated whether ethanol extract of MC in the presence of H2O2 attenuated cell death in human neuroblastoma SH-SY5Y cells. MC improved H2O2-stimulated intracellular reactive oxygen species (ROS) production. Additionally, MC reduced caspase activation, which greatly improved cell viability and avoided H2O2-induced apoptosis. Through its anti-oxidant and anti-apoptotic properties, MC ethanol extract has been shown to be effective in protecting against H2O2-induced cell death.

Keywords

Hydrogen peroxide, Oxidative stress, Apoptosis

Supporting Institution

Erciyes University Scientific Research Projects Unit

Project Number

TYL-2022-11887

Thanks

A part of this study was carried out at Erciyes University Betül Ziya Eren Genome and Stem Cell Center in 2022. We thank Kardelen Azgın (M.Sc. student) for help in the experiments.

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