Cytotoxic activities on selected Lamiaceae Species from Turkey by brine shrimp lethality bioassay

Yıl 2019, Cilt: 9 Sayı: 2, 105 - 111, 31.12.2019

Arzu Kaska

Öz

In this study, an experiment for cytotoxic activity
was carried out on the methanol and water extracts of three species belonging
to the Lamiaceae family (Thymus zygioides
Griseb, Teucrium sandrasicum, Origanum sipyleum) collected from Turkey. A
brine shrimp (Artemia salina L.)
lethality test was established for the present study and the cytotoxicity was
reported in terms of 50 % lethal concentration (LC₅₀). The LC₅₀
values of the extracts were calculated using an EPA Probit Analysis Program
(version 1.5). The A. salina were
hatched and the active shrimps were collected for use in the assay. Ten A. salina were added to different
concentrations of the extracts, the surviving shrimps were counted after a
period of 24 h and the lethality concentration LC₅₀ was assessed.
The water and methanol extracts of these plants showed cytotoxicity against
brine shrimp. The cytotoxic activities of the extracts ranged from
34.66-643.652 µg/mL. The strongest cytotoxic activity were obtained from the
methanol extract of T. zygioides.
This significant lethality of the extracts from three species could be the
source of potential cytotoxic components in these species but this will require
further investigation.

Anahtar Kelimeler

Artemia salina, cytotoxic, Origanum sipyleum, Thymus zygioides

Kaynakça

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