The Detection of Fractal Dimension with Protective Effects of Lycopene and Catechin Carbon Tetrachloride-Treated Rat's Liver

Yıl 2015, Cilt: 37 Sayı: 1, 14 - 21, 05.11.2015

Hülyam Kurt , Hilmi Özden , Bünyamin Demir Mustafa Saltan , Derya Üstüner , Dilek Burukoğlu , Mehmet Üstüner , İrfan Değirmenci

https://doi.org/10.20515/otd.60303

Öz

Our aim is to investigate the detection of fractal dimension (Box-counting dimensions) with protective effects of lycopene and catechin in carbon tetrachloride-treated rat's liver. Fractal dimension measure intensity and complexity of subject. The rats were divided into six groups as liquid oil, lycopene, catechin, carbon tetrachloride plus lycopene, carbon tetrachloride plus catechin and carbon tetrachloride. The tissue samples were obtained at end of 2İst day. The liver tissue distortions were evaluated using hematoxylin and eosin staining. The regions of hepatocyte were separated into Small Square under light microscopy. Then, counted at least one nucleus with intersection of one square. Fractal dimension were determined by using this formula; ln(N(n))/ln(2n). Notably, there was no difference between control and catechin-treated rats on fractal dimension values. But, a lycopene value was bigger than control and catechin administrated rats. In carbon tetrachloride-treated rat, fractal dimension is less. It was increased in lycopene plus carbon tetrachloride and catechin plus carbon tetrachloride with compare to carbon tetrachloride group. The findings confirmed, if hepatocyte is less, fractal dimension will decrease On other hand, carbon tetrachloride decreased fractal dimension due to less hepatocyte. It was increased in fractal dimension due to high hepatocyte in catechin and lycopene treated rats, because of their protective effects. This study is combination of medicine and mathematics, as fractal dimension. It was calculated, toxicity and protective effects of antioxidant materials could be determined in this way.

Anahtar Kelimeler

CCl4, Lycopene, catechin, Fractal dimension

The Detection of Fractal Dimension with Protective Effects of Lycopene and Catechin Carbon Tetrachloride-Treated Rat's Liver

Öz

Amacımız karbon tetraklorür uygulanmış sıçan karaciğerinde likopen ve kateşinin koruyucu etkilerinin fraktal boyut hesaplaması kullanılarak ortaya konmasıdır. Fraktal boyut nesnenin yoğunluğunu ve karmaşıklığını ölçer. Sıçanlar; sıvı yağ, likopen, kateşin, karbon tetraklorür + likopen, karbon tetraklorür + kateşin ve karbon tetraklorür olmak üzere altı gruba ayrıldı. Doku örnekleri 21. gün sonunda elde edildi. Karaciğer dokusundaki harabiyet, hematoksilen ve eozin boyama ile değerlendirildi. Hepatosit bölgeleri ışık mikroskobu altında küçük karelere ayrıldı. Sonra en az bir nükleusun bir kareyle kesiştiği alan sayıldı. Fraktal boyut ln(N(n))/ln(2n) formülle hesaplandı. Özellikle, fraktal boyut değerleri açısından kontrol ve kateşin ile tedavi edilen gruplar arasında bir fark yoktu. Ama likopen değerleri kontrol ve kateşin uygulanan sıçanlarda daha yüksek bulundu. Karbon tetraklorür uygulanmış sıçanlarda fraktal boyut düşüktü. Karbon tetraklorür uygulanmış gruba göre karbon tetraklorür + likopen ve karbon tetraklorür + kateşin gruplarında fraktal boyutta artış gözlendi. Bulgular hepatosit miktarındaki azalmanın, fraktal boyutun azalmaya neden olacağını göstermiştir. Diğer taraftan, karbon tetraklorür hepatositlerin az olması nedeniyle fraktal boyutu azaltmıştır. Kateşin ve likopen uygulanan gruplarda yüksek hepatosit miktarına bağlı olarak fraktal boyutta artış görüldü. Bu çalışma fraktal boyut kullanılarak tıp ve matematiği birleştirmektedir. Bu hesaplamalar doğrultusunda, antioksidan maddelerin toksisite ve koruyucu etkisi tespit edilebilir.

Anahtar Kelimeler

CCl4, likopen, kateşin, fraktal boyut

Kaynakça

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