Orta yaş sıçanlarda gün aşırı açlık protokolünün vücut ağırlığı, oksidatif stres ve metabolik sağlık üzerine cinsiyete özgü etkileri

Yıl 2024, Cilt: 17 Sayı: 4, 690 - 701, 09.10.2024

Özgen Kılıç Erkek , Gülşah Gündoğdu

https://doi.org/10.31362/patd.1487708

Öz

Amaç: Bu çalışmada, gün aşırı açlık protokolünün (ADF) cinsiyete özgü etkisinin ve yaşlanma ile ilişkili oksidatif
stres değişimlerindeki işlevlerinin değerlendirilmesi amaçlandı.
Gereç ve yöntem: Kırk iki dişi (n=21) ve erkek (n=21) 16 aylık Wistar sıçanları altı gruba (n=7) ayrıldı: Grup-1
(kontrol-erkek), Grup-2 (1 ay, ADF-erkek), Grup-3 (2 ay, ADF-erkek), Grup-4 (kontrol-dişi), Grup-5 (1 ay, ADFdişi)
ve Grup-6 (2 ay, ADF-dişi). ADF protokolü günaşırı 24 saatlik oruç tutma şeklinde uygulandı (haftada üç
gün). Serum örnekleri, ELISA yöntemiyle toplam oksidan-antioksidan seviyelerini (TOS-TAS) ölçmek için alındı
ve oksidatif stres indeksi (OSI) hesaplandı.
Bulgular: İki aylık-ADF tedavisinin kümültatif kontrol gruplarıyla karşılaştırıldığında vücut ağırlığında (VA)
anlamlı azalma tespit edildi (p<0,001). Tüm gruplarda kümülatif gıda alımının ve retroperitoneal yağ ağırlığının
ADF ile azaldığı görüldü (p<0,05). Hem 1 aylık hem de 2 aylık ADF uygulanması karaciğer ve serumda TOS
seviyesi ve OSI'yi azaltmada olumlu etkiler gösterdi ve her iki grup da kendi kontrollerine göre anlamlı bir
azalma gözlemlendi (p<0,001). Karaciğer TAS seviyesi dişi sıçanlarda anlamlı olarak arttı (p<0,05), ancak
erkek sıçanlarda bu artış anlamlı bir seviyeye ulaşmadı. Gruplar arasındaki serum TAS seviyesinde anlamlı fark
saptanmadı.
Sonuç: Bu çalışma, erkek ve dişi sıçanlarda ADF'nin VA, gıda tüketimi ve oksidatif stres parametreleri
üzerindeki etkilerini değerlendirdi. Bulgular, ADF'nin kilo yönetimi ve oksidatif stresi azaltmada potansiyel
faydalarını vurgulamaktadır. Sonuç olarak, ADF'nin metabolik sağlık üzerindeki etkilerini anlamada ve olası
klinik uygulamaları belirlemede önemli bir adımı temsil etmektedir.

Anahtar Kelimeler

Yaşlanma, alternatif günlerde açlık, gıda alımı, cinsiyet, oksidatif stres

Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats

Öz

Purpose: The purpose of this study was to assess the effect of alternate-day fasting (ADF) concerning sex as
well as its function in systemic and tissue-level oxidative stress alterations associated with aging.
Materials and methods: Forty-two female (n=21) and male (n=21) Wistar rats (aged 16 months) were separated
into six groups (n=7 each): Group-1 (control-male), Group-2 (1-month, ADF-male), Group-3 (2-month, ADFmale),
Group-4 (control-female), Group-5 (1-month, ADF-female), and Group-6 (2-month, ADF-female). The
ADF protocol was applied every other day for 24-h of fasting (three days/week). Serum samples were analyzed
via ELISA to measure total oxidant-antioxidant status (TOS-TAS), and the oxidative stress index (OSI) was
calculated.
Results: 2-months of ADF treatment reduced body weight (BW) compared compliance control groups (p<0.001).
All groups' cumulative food intake and retroperitoneal fat weight decreased with ADF (p<0.05). Both 1-month
and 2-month ADF interventions had positive effects on reducing TOS and OSI in both liver and serum, with a
significant decrease observed in both groups compared to their respective controls (p<0.001). The liver TAS
significantly increased in female rats (p<0.05), but this increase did not reach a significant level in male rats. The
difference in the serum TAS between the groups was not significant.
Conclusions: This study evaluated the effects of ADF on BW, food consumption, and oxidative stress
parameters in male and female rats. The findings highlight ADF's potential benefits in weight management
and reducing oxidative stress. This study represents an important step in understanding the effects of ADF on
metabolic health and in identifying potential clinical applications.

Anahtar Kelimeler

Aging, alternate-day fasting, food intake, gender, oxidative stress

Etik Beyan

Animal Experiments were approved by the Medical Ethics Committee of Pamukkale University (dated 15.12.2023 and numbered PAUHADYEK-2023/60758568-020-468703).

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