ERKEK SIÇANLARDA AROKLOR 1254‘ÜN NEDEN OLDUĞU REPRODUKTİF HASARA KARŞI TETRANDRİNİN KORUYUCU ETKİLERİNİN ARAŞTIRILMASI

Yıl 2023, , 80 - 87, 28.02.2023

Feride Özdemir , Aslı Çetin , Osman Çiftçi , Muhterem Aydın , Neşe Başak Türkmen

https://doi.org/10.26650/JARHS2023-1181839

Öz

Amaç: Aroklor (AR) 1254; Kanserojenik, endokrin bozucu, nörotoksik, immünsüpresif, teratojenik etkileri olan polilklorlu bifenil bileşiğidir. Stephania tetrandra S. Moore’un kökünden izole edilen bir bisbenzillizokinolin alkaloid olan Tetrandrin (TET) ise immünomodülatör, anti kanser, anti-inflamatuar etkilere sahiptir. Bu çalışma, AR1254’ün neden olduğu testis hasarına (histolojik, spermatolojik ve oksidatif hasar) karşı TET tedavisinin koruyucu, iyileştirici etkilerini göstermek amacıyla planlandı. Materyal ve Metot: Çalışmamızda 28 adet Sprauge-Dawley cinsi sıçanlar kullanıldı ve rastgele seçilerek 4 gruba ayrıldı. (n=7) Gruplar sırasıyla: Kontrol grubu; (1 ml mısır yağı gastrik gavaj ile), AR1254; (2 mg/kg/gün intraperitonal yolla), TET grubu 30 mg/kg/gün gastrik gavaj ile), AR1254+TET grubu; Aroclor1254 ve TET grubuna uygulan dozlar aynı şekilde uygulanarak oluşturuldu. Deney süresi sonunda sıçanlardan alınan testis dokuları histopatolojik ve biyokimyasal yöntemlerle analiz edildi. Bulgular: AR1254 uygulaması sonucunda sıçan testis dokusunda interstisyel alanda; vakuolizasyon, ödem, vasküler konjesyon, spermatogenik hücrelerde; azalma, bölünmenin herhangi bir evresinde duraksama ve lümene dökülme, epididimal sperm konsantrasyonunda, sperm hareketliliğinde azalma ve anormal sperm oranlarında ise artış gözlendi. AR1254 uygulaması oksidatif stres parametrelerinde artışa ve buna karşılık antioksidan seviyelerinde ise azalışa sebep oldu. Uygulanan TET tedavisi, AR1254’ün neden olduğu histolojik, oksidatif ve sperm hasarını önemli ölçüde iyileştirdi. Sonuç: Bu çalışmada, erkek sıçanlarda AR1254 ile oluşturulan testis dokusu hasarına karşı TET’in koruyucu ve iyileştirici etkileri gösterildi.

Anahtar Kelimeler

Aroklor1254, testis, tetrandrin, sıçan, kaspaz-3

Destekleyen Kurum

İnönü Üniversitesi Bilimsel Araştırma Proje Birimi

Proje Numarası

2013/11

Teşekkür

İnönü Üniversitesi Bilimsel Araştırma Proje Birimi 'ne katkılarından dolayı teşekkür ederiz

THE PROTECTIVE EFFECTS OF TETRANDRINE AGAINST TO HISTOLOGICAL, SPERMATOLOGICAL AND OXIDATIVE DAMAGE INDUCED BY AROCLOR 1254 ON THE MALE RATS REPRODUCTIVE SYSTEM

Öz

Objectives: Aroclor (AR) 1254; has many adverse effects on male reproduction such as carcinogenic, teratogenic, immune and endocrine disruption problems. Tetrandrine (TET), a bisbenzillisoquinoline alkaloid isolated from the root of Stephania tetrandra S. Moore, has protective effects such as immunomodulatory, anti-cancer, and anti-inflammatory. The objective of this study was to investigate the possible curative effects of TET therapy against testicular damage (histological, spermatological and oxidative damage) induced by AR1254. Materials and Methods: Twenty-eight male rats were randomly divided into four equal-sized groups: a control group; (1 ml of corn oil by gastric oral gavage), AR1254 group; (2 mg/kg) AR1254 administered intraperitoneally), TET group; (TET by gastric oral gavage 30 mg/kg) and AR 1254 + TET group;(Aroclor 1254 and TET administered together at the same doses as the previous groups. Results: The AR1254 treatment caused morphological and spermatological damage on testis tissue; oedema vacuolization and congestion, in interstitial area, reduction in spermatogenic cells, arrested spermatocytes at different stages of spermatogenesis, shedding of spermatogenic serial cells into tubular lumens, a decline in epididymal sperm concentrations, sperm motility and a rise in abnormal sperm ratios. The AR1254 administration induced an increase in the oxidative parameters and a decrease in enzymatic and nonenzymatic antioxidant levels. The TET treatment significantly ameliorated histological, oxidative, and sperm damage caused by AR1254. Conclusion: This study demonstrated the protective effects of TET against AR1254-induced male rat reproductive damage.

Anahtar Kelimeler

Aroclor1254, testis, tetrandrine, rat, caspase-3

Proje Numarası

2013/11

Kaynakça

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