ÜÇÜNCÜ TÜREV UV SPEKTROFOTOMETRİ İLE TABLETLERDE MELOKSİKAM TAYİNİ

Yıl 2024, , 61 - 67, 15.02.2024

Zeynep Aydoğmuş , Faruk Alim

https://doi.org/10.26650/JARHS2024-1238611

Öz

Amaç: Meloksikam (MEL), analjezik ve antipiretik etkileri olan seçici bir siklooksijenaz inhibitörü enolik asit sınıfı bir ilaçtır. Bu çalışmada, tablet formülasyonunda meloksikam tayini için kolay, seçici, hızlı ve hassas bir üçüncü dereceden türev spektrofotometrik yöntem geliştirilmiş ve valide edilmiştir.

Gereç ve Yöntemler: Üçüncü türev spektrometrisi ile ilaç tayini için en uygun bulunan metanol-1 M sodyum hidroksit (1:1, v/v) çözeltisinde meloksikamın absorpsiyonu pik-sıfır yöntemi ile okundu. Ölçümlerde maksimum absorpsiyonun elde edildiği dalga boyu 341 nm idi. Geliştirilen yöntem, Uluslararası Uyumlaştırma Kılavuzuna (ICH) uygun olarak valide edilmiştir.

Bulgular: Doğrusal çalışma aralığı 1,0 - 14,0 μg/mL idi. Gözlenebilme sınırı (LOD) ve tayin sınırı (LOQ) değerleri sırasıyla 0,22 ve 0,75 μg/mL idi. Yöntem, doğrusallık, doğruluk, kesinlik, geri kazanım ve kararlılık açısından doğrulandı. Geliştirilen yöntem tabletlerde meloksikam miktar tayinine uygulanmış ve geri kazanım yüzdesi %97,50 ile %98,12 arasında bulunmuştur.

Sonuç: Sonuçlar, yöntemin doğru ve duyarlı olmasının yanı sıra, yayınlanmış diğer yöntemlere göre kolay, basit, ucuz ve hızlı olduğunu göstermektedir. Önerilen yöntem, kalite kontrolde rutin analizlerde farmasötik formülasyonlarda meloksikam tayini için çok uygun bir alternatif olarak kullanılabilir.

Anahtar Kelimeler

Üçüncü türev spektrofotometrisi, tayin, meloksikam, tablet

Proje Numarası

Project number 37460-1150.

DETERMINATION OF MELOXICAM IN TABLETS BY THIRD DERIVATIVE UV SPECTROPHOTOMETRIC METHOD

Öz

Objectives: Meloxicam (MEL) is a selective cyclooxygenase inhibitor of enolic acid class drugs with analgesic and antipyretic effects. In this study, an easy, selective, fast, and sensitive third-order derivative spectrophotometric method was developed and validated for the determination of MEL in tablet formulation.

Material and Methods: The absorption of MEL in a solution of methanol and 1 M sodium hydroxide (1:1, v/v) mixture was measured using the peak-to-zero method. This solution was found to be the most suitable for determining the drug by third-order derivative spectrometry. The wavelength at which the maximum absorption was achieved in the measurements was 341nm. The developed method has been validated in accordance with the International Conference on Harmonization guidelines (ICH).

Results: The linear working range was 1.0 - 14.0 µg/mL. The limit of detection (LOD) and limit of quantification (LOQ) values were 0.22 and 0.75 μg/mL, respectively. The method was validated for linearity, accuracy, precision, recovery, and stability. The developed method was performed for the quantification of MEL in tablets, and the recovery percentage was found to be between 97.50% and 98.12%.

Conclusion: The results show that the method is easy, simple, inexpensive, and fast compared to other published methods, in addition to being accurate and sensitive. The proposed method can be used as a very convenient alternative for the determination of MEL in pharmaceutical formulations in routine analysis in quality control.

Anahtar Kelimeler

Third-derivative spectrophotometry, determination, meloxicam, tablets

Destekleyen Kurum

Scientific Research Project Coordination Unit of Istanbul University

Proje Numarası

Project number 37460-1150.

Teşekkür

The authors would like to thank the Scientific Research Project Coordination Unit of Istanbul University for their support of their work with Project number and ID 37460 and 1150, respectively.

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