Testing The Biodegradability of Priority And Emerging Contaminants As A Mixture

Yıl 2019, Cilt: 23 Sayı: 2, 184 - 192, 01.04.2019

Ulas Tezel , Begüm Şepitci

https://doi.org/10.16984/saufenbilder.413597

Öz

Currently about 110,000 chemical substances are present in the European market. The fate of most of those chemicals in the environment is not known. However, biodegradability of those chemicals should be tested before they are registered to the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) System. Current protocols offered by The Organisation for Economic Co-operation and Development (OECD) for testing the biodegradability of the chemicals are limited mainly due to they are low throughput and do not reflect real-world conditions. In OECD protocols, the biodegradability of a single chemical is tested. However, many chemicals coexist in the environment. In addition, experiments are set at a very high initial chemical concentration that is not expected in the environment. Both limitations are due to the lack of an analytical method which can measure multiple compounds simultaneously at very low concentrations. In this study, we coupled OECD 314 Simulation Tests to Assess the Biodegradability of Chemicals Discharged in Wastewater protocol with a powerful liquid chromatography mass spectrometry with scheduled multiple reaction monitoring and tested the biodegradability of 32 priority substances and chemicals with emerging concern. Only seven chemicals were degraded in the test within 28 days. The biodegradation half-lives of those degradable chemicals ranged between 0.6 to 18 days. Acetaminophen was degraded the fastest whereas biodegradation of sulfamethoxazole took longer than the rest of the biodegradable chemicals tested. The novel methodology described here can be applied to test biodegradability of different chemicals as a mixture and adopted as a standard protocol. 

Anahtar Kelimeler

biodegradability, biodegradation testing, chemicals of emerging concern, priority substances, priority substances

Kaynakça

• L. Jones, J. Ronan, B. McHugh, E. McGovern, and F. Regan, "Emerging priority substances in the aquatic environment: a role for passive sampling in supporting WFD monitoring and compliance," Analytical Methods, vol. 7, pp. 7976-7984, 2015.
• K. Fent, A. A. Weston, and D. Caminada, "Ecotoxicology of human pharmaceuticals," Aquatic Toxicology, vol. 76, pp. 122-159, 2006.
• A. Pruden, R. T. Pei, H. Storteboom, and K. H. Carlson, "Antibiotic resistance genes as emerging contaminants: Studies in northern Colorado," Environmental Science & Technology, vol. 40, pp. 7445-7450, 2006.
• K. Czechowska, V. Sentchilo, S. Beggah, S. Rey, M. Seyfried, and J. R. van der Meer, "Examining Chemical Compound Biodegradation at Low Concentrations through Bacterial Cell Proliferation," Environmental Science & Technology, vol. 47, pp. 1913-1921, 2013.
• OECD, Test No. 301: Ready Biodegradability: OECD Publishing, 1992.
• OECD, Test No. 302A: Inherent Biodegradability: Modified SCAS Test: OECD Publishing, 1981.
• OECD, Test No. 314: Simulation Tests to Assess the Biodegradability of Chemicals Discharged in Wastewater: OECD Publishing, 2008.
• A. Kowalczyk, T. J. Martin, O. R. Price, J. R. Snape, R. A. van Egmond, C. J. Finnegan, et al., "Refinement of biodegradation tests methodologies and the proposed utility of new microbial ecology techniques," Ecotoxicology and Environmental Safety, vol. 111, pp. 9-22, 2015.
• T. J. Martin, A. K. Goodhead, K. Acharya, I. M. Head, J. R. Snape, and R. J. Davenport, "High Throughput Biodegradation-Screening Test To Prioritize and Evaluate Chemical Biodegradability," Environmental Science & Technology, 2017.
• B. De Gusseme, L. Vanhaecke, W. Verstraete, and N. Boon, "Degradation of acetaminophen by Delftia tsuruhatensis and Pseudomonas aeruginosa in a membrane bioreactor," Water Research, vol. 45, pp. 1829-1837, 2011.
• J. Hu, L. L. Zhang, J. M. Chen, and Y. Liu, "Degradation of paracetamol by Pseudomonas aeruginosa strain HJ1012," Journal of Environmental Science and Health Part a-Toxic/Hazardous Substances & Environmental Engineering, vol. 48, pp. 791-799, 2013.
• S. J. Wu, L. L. Zhang, and J. M. Chen, "Paracetamol in the environment and its degradation by microorganisms," Applied Microbiology and Biotechnology, vol. 96, pp. 875-884, 2012.
• K. A. Shin and J. C. Spain, "Pathway and Evolutionary Implications of Diphenylamine Biodegradation by Burkholderia sp Strain JS667," Applied and Environmental Microbiology, vol. 75, pp. 2694-2704, 2009.
• C. Christodoulatos, A. D. Koutsospyros, B. W. Brodman, and G. P. Korfiatis, "Biodegradation of diphenylamine by selected microbial cultures," Journal of Environmental Science and Health Part A-Environmental Science and Engineering & Toxic and Hazardous Substance Control, vol. 32, pp. 15-30, 1997.
• Y. Yamazaki, Y. Hayashi, M. Arita, T. Hieda, and Y. Mikami, "Microbial Conversion of alpha-Ionone, alpha-Methylionone, and alpha-Isomethylionone," Applied Environmental Microbiology, vol. 54, pp. 2354-60, 1988.
• R. N. Fathulla, "Aerobic Aquatic Metabolism of 14C-Pyriproxyfen," Sumitomo Chemical Company1993.
• S. Larcher and V. Yargeau, "Biodegradation of sulfamethoxazole: current knowledge and perspectives," Applied Microbiology and Biotechnology, vol. 96, pp. 309-318, 2012.
• P. Y. Nguyen, G. Carvalho, A. C. Reis, O. C. Nunes, M. A. M. Reis, and A. Oehmen, "Impact of biogenic substrates on sulfamethoxazole biodegradation kinetics by Achromobacter denitrificans strain PR1," Biodegradation, vol. 28, pp. 205-217, 2017.
• J. Birkigt, T. Gilevska, B. Ricken, H. H. Richnow, D. Vione, P. F. X. Corvini, et al., "Carbon Stable Isotope Fractionation of Sulfamethoxazole during Biodegradation by Microbacterium sp Strain BR1 and upon Direct Photolysis," Environmental Science & Technology, vol. 49, pp. 6029-6036, 2015.
• B. C. Jiang, A. Li, D. Cui, R. Cai, F. Ma, and Y. N. Wang, "Biodegradation and metabolic pathway of sulfamethoxazole by Pseudomonas psychrophila HA-4, a newly isolated cold-adapted sulfamethoxazole-degrading bacterium," Applied Microbiology and Biotechnology, vol. 98, pp. 4671-4681, 2014.
• A. Barra Caracciolo, P. Grenni, R. Ciccoli, G. Di Landa, and C. Cremisini, "Simazine biodegradation in soil: analysis of bacterial community structure by in situ hybridization," Pest Management Science, vol. 61, pp. 863-869, 2005.
• M. Blaszak, R. Pelech, and P. Graczyk, "Screening of Microorganisms for Biodegradation of Simazine Pollution (Obsolete Pesticide Azotop 50 WP)," Water Air and Soil Pollution, vol. 220, pp. 373-385, 2011.
• T. Kodama, L. X. Ding, M. Yoshida, and M. Yajima, "Biodegradation of an s-triazine herbicide, simazine," Journal of Molecular Catalysis B-Enzymatic, vol. 11, pp. 1073-1078, 2001.
• R. Wan, Y. Y. Yang, W. M. Sun, Z. Wang, and S. G. Xie, "Simazine biodegradation and community structures of ammonia-oxidizing microorganisms in bioaugmented soil: impact of ammonia and nitrate nitrogen sources," Environmental Science and Pollution Research, vol. 21, pp. 3175-3181, 2014.